An Antisense RNA-mediated Mechanism Eliminates a Meiosis-specific Copper-regulated Transcript in Mitotic Cells

Normant, Vincent; Beaudoin, Jude; Labbé, Simon

doi:10.1074/jbc.m115.674556

Cited by 3 publications

(2 citation statements)

References 51 publications

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“…Here, we used ctr4 + as a control gene known to be induced under copper-limiting conditions ( Fig 4A ). Furthermore, we performed ChIP assays using Cuf1-Myc 12 as a control transcription factor known to interact with the ctr4 + promoter in vivo under low copper conditions ( Fig 4B and 4C ) [ 43 ]. In the case of the mfc1 + transcription profile, there was no detection of the mfc1 + transcript in mitotically growing cells in the presence or absence of TTM or CuSO 4 ( Fig 4A ) [ 6 ].…”

Section: Resultsmentioning

confidence: 99%

A role for the transcription factor Mca1 in activating the meiosis-specific copper transporter Mfc1

et al. 2018

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When reproduction in fungi takes place by sexual means, meiosis enables the formation of haploid spores from diploid precursor cells. Copper is required for completion of meiosis in Schizosaccharomyces pombe. During the meiotic program, genes encoding copper transporters exhibit distinct temporal expression profiles. In the case of the major facilitator copper transporter 1 (Mfc1), its maximal expression is induced during middle-phase meiosis and requires the presence of the Zn6Cys2 binuclear cluster-type transcription factor Mca1. In this study, we further characterize the mechanism by which Mca1 affects the copper-starvation-induced expression of mfc1+. Using a chromatin immunoprecipitation (ChIP) approach, results showed that a functional Mca1-TAP occupies the mfc1+ promoter irrespective of whether this gene is transcriptionally active. Under conditions of copper starvation, results showed that the presence of Mca1 promotes RNA polymerase II (Pol II) occupancy along the mfc1+ transcribed region. In contrast, Pol II did not significantly occupy the mfc1+ locus in meiotic cells that were incubated in the presence of copper. Further analysis by ChIP assays revealed that binding of Pol II to chromatin at the chromosomal locus of mfc1+ is exclusively detected during meiosis and absent in cells proliferating in mitosis. Protein function analysis of a series of internal mutants compared to the full-length Mca1 identified a minimal form of Mca1 consisting of its DNA-binding domain (residues 1 to 150) fused to the amino acids 299 to 600. This shorter form is sufficient to enhance Pol II occupancy at the mfc1+ locus under low copper conditions. Taken together, these results revealed novel characteristics of Mca1 and identified an internal region of Mca1 that is required to promote Pol II-dependent mfc1+ transcription during meiosis.

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Section: Resultsmentioning

confidence: 99%

A role for the transcription factor Mca1 in activating the meiosis-specific copper transporter Mfc1

et al. 2018

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“…In fission yeast, several protein-coding genes are regulated by antisense small RNAs [123]. In RNAi mutants, they are typically up-regulated at the protein level, but not at the mRNA level, indicating that RNAi can effect translational inhibition in fission yeast [123], although some genes (such as cum1) are regulated at the mRNA level [124]. However, while many loci can give rise to antisense transcription, such as during sexual differentiation, they are not targeted by RNAi [125], indicating that specific recruitment mechanisms must be present to allow RNAi to degrade the transcript or inhibit its translation.…”

Section: The Role Of the Rits Complex In Post-transcriptional Gene Simentioning

confidence: 99%

RNA-induced initiation of transcriptional silencing (RITS) complex structure and function

2019

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Heterochromatic regions of the genome are epigenetically regulated to maintain a heritable '"silent state"'. In fission yeast and other organisms, epigenetic silencing is guided by nascent transcripts, which are targeted by the RNA interference pathway. The key effector complex of the RNA interference pathway consists of small interfering RNA molecules (siRNAs) associated with Argonaute, assembled into the RNA-induced transcriptional silencing (RITS) complex. This review focuses on our current understanding of how RITS promotes heterochromatin formation, and in particular on the role of Argonaute-containing complexes in many other functions such as quelling, release of RNA polymerases, cellular quiescence and genome defense.

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Iron homeostasis proteins Grx4 and Fra2 control activity of the Schizosaccharomyces pombe iron repressor Fep1 by facilitating [2Fe-2S] cluster removal

Hati,

Brault,

Gupta

et al. 2023

Journal of Biological Chemistry

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An Antisense RNA-mediated Mechanism Eliminates a Meiosis-specific Copper-regulated Transcript in Mitotic Cells

Abstract: Background: Convergent gene pairs produce sense and antisense transcripts that could interfere with one another. Results: RNAi, sense, and antisense transcripts play a causal role in cum1 ϩ gene silencing in vegetative cells.

Cited by 3 publications

References 51 publications

A role for the transcription factor Mca1 in activating the meiosis-specific copper transporter Mfc1

A role for the transcription factor Mca1 in activating the meiosis-specific copper transporter Mfc1

RNA-induced initiation of transcriptional silencing (RITS) complex structure and function

Iron homeostasis proteins Grx4 and Fra2 control activity of the Schizosaccharomyces pombe iron repressor Fep1 by facilitating [2Fe-2S] cluster removal

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