An antioxidant hispidin from the mycelial cultures ofPhellinus linteus

Abstract

In the course of screening for reactive oxygen species scavengers from natural products, an antioxidant was isolated from the mycelial culture broth of Phellinus linteus and identified as hispidin. The hispidin content was reached its maximum level at 12 days after onset of inoculation. About 2.5 mg/mL of hispidin was produced by P. linteus in a yeast-malt medium (pH 5.8, 25 degrees C). Hispidin inhibited 22.6 and 56.8% of the super oxide anion radical, 79.4 and 95.3% of the hydroxyl radical, and 28.1 and 85.5% of the DPPH radical at 0.1 and 1.0 mM, respectively. The positive control alpha-tocopherol scavenged 25.6 and 60.3%, 74.6 and 96.3%, and 32.7 and 77.5% of each radical, respectively, at the same concentrations. However, hispidin showed no significant activity on the hydrogen peroxide radical.

Styrylpyrone-class compounds from medicinal fungi Phellinus and Inonotus spp., and their medicinal importance

“…Nevertheless, the cultivated mushroom or cultured mycelia of P. linteus exhibited biological activities comparable to the wild-grown mushroom, and produced an abundant amount of yellow pigments containing mainly styrylpyrones with various biological activities. 28,29,34,[52][53][54][55][56][57]60,64,[72][73][74][75] In addition, the adverse effects of the medicinal mushrooms Phellinus and Inonotus have not been reported until now. Methanolic extracts of I. xeranticus did not show acute toxicity up to 2 g kg À1 in mice, and the LD 50 values of these extracts were above 2 g kg À1 , indicating the safe nature of these mushrooms.…”

“…[38][39][40] Phelligridins C (19) and D (20) were then isolated from P. linteus and reported as meshimakobnols A and B, respectively. 41 In addition, the fruiting body of P. linteus was also reported to produce phellifuropyranone A (27) with anti-proliferative activity against mouse melanoma and human lung cancer cells in vitro, 42 and two novel furan derivatives, phellinusfurans A (28) and B (29), which displayed anticomplement activity (Figure 3). 43 Phellifuropyranone A (27) was concurrently reported as inoscavin E (32), which was isolated from the fruiting bodies of I. xeranticus.…”

“…27 Compound 1 from mycelial cultures of P. linteus exhibited anti-oxidative and b-secretase inhibitory activities. 28,29 Remarkably, various hispidin-classes of metabolites have been biogenerated by dimerization or oligomerization of styrylpyrone building blocks (Figure 1). Hispidin (1) and bisnoryangonin (2) may polymerize during maturation of fungi and production of fungal lignin.…”

Inhibition of human neutrophil elastase by ergosterol derivatives from the mycelium of Phellinus linteus

“…[13][14][15][16] However, there is little information on compounds of the mycelium of P. linteus, with the exception of polysaccharides.…”

“…The CHCl 3 -soluble fraction exhibited significant HNE-inhibition, with an IC 50 of 5.18 mg ml À1 . Further phytochemical studies of this fraction resulted in the isolation of a new ergosterol derivative, (22E,24R)-ergosta-7,22-dien-2a,3a,9a-triol (1) together with 14 known compounds (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) (Figure 1). This report describes the isolation and structural elucidation of these ergosterol derivatives, as well as the characterization of their inhibitory effects on HNE and some interesting structural requirements for their activity.…”