An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine- Induced Hepatotoxicity in Rats

Çatal, Tunç; Tunalı, Sevim; Bolkent, Şehnaz; Yanardağ, Refiye

doi:10.5152/eurjbiol.2017.1703

Cited by 6 publications

(6 citation statements)

References 45 publications

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“…In our work, intraperitoneal injection of 500 mg·kg −1 d ‐GalN resulted in serious hepatocellular injury. The impairment was exhibited as escalation of hepatic enzymes, along with histopathological alterations in the liver tissue of the d ‐GalN‐induced animals, as discussed in our preceding work …”

Section: Discussionsupporting

confidence: 58%

“…In the antioxidant network system, antioxidant vitamins (ascorbic acid, β‐carotene, α‐tocopherol, and vitamin U) and antioxidant metals/elements (selenium, vanadium, and zinc), respectively, scavenge free radical and act as enzymes' cofactors . Ascorbic acid, β‐carotene, α‐tocopherol as well as selenium presumably deter oxidation/breakdown of neuronal membranes . In general, antioxidants stabilize cell membranes (particularly, neuronal membranes) by counteracting lipid peroxidation .…”

Section: Introductionmentioning

confidence: 99%

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The effects of vitamins and selenium mixture against brain tissue induced byd‐galactosamine

Tunalı

Çatal

Bolkent

et al. 2019

J Biochem & Molecular Tox

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Brain damage is a major complication of fulminant hepatic failure. d‐Galactosamine (d‐GalN)‐induced liver toxicity causes damage to brain. The effects of vitamins and selenium mixture against d‐GalN stimulated brain injury were investigated in this study. Sprague‐Dawley female rats aged 2.0‐2.5 months were used for the study. The rats were divided into four categories. A 0.9% NaCl solution was intraperitoneally given to the experimental rats in the first group. Using gavage technique, the second group of animals were subjected to a formulation consisting of 100 mg·kg−1·day−1 vitamin C, 15 mg·kg−1·day−1 of β‐carotene, 100 mg·kg−1·day−1 of α‐tocopherol in addition to 0.2 mg·kg−1·day−1 of sodium selenate for 3 days. The third group was given a single dose of d‐GalN hydrochloride at the concentration of 500 mg·kg−1 through a saline injection. The final group was given similar concentrations of both the antioxidant combination and d‐GalN. Tissue samples were collected under ether anesthesia. The rats treated with d‐GalN showed brain damage; increased myeloperoxidase, catalase, glutathione peroxidase, glutathione‐S‐transferase, lactate dehydrogenase, and superoxide dismutase activities; and decreased glutathione levels. Treatment with vitamins and selenium combination resulted in alleviation of these alterations in the rats. These findings suggest that administration of the vitamins and selenium combination suppresses oxidative stress and protects brain cells from injury induced by d‐GalN.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

The effects of vitamins and selenium mixture against brain tissue induced byd‐galactosamine

Tunalı

Çatal

Bolkent

et al. 2019

J Biochem & Molecular Tox

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“…It was previously suggested that p53 stimulate BAD and promote apoptosis through cytochrome c release from mitochondria in cancer cells (33). Protective effects of selenium and quercetin with other antioxidants were previously reported in biological systems (34)(35)(36)(37)(38)(39). The combination of both molecules in chemotherapy treatment may reduce the damage and accelerate the repairing process caused by chemotherapy.…”

Section: Discussionmentioning

confidence: 81%

Synergistic effects of quercetin and selenium on oxidative stress in endometrial adenocarcinoma cells

Cebecioglu¹,

Yıldırım²,

Akagunduz³

et al. 2019

BLL

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OBJECTIVE: The effects of quercetin and selenium on oxidative stress in endometrial adenocarcinoma cells are unclear. In this study, the effects of quercetin and selenium on oxidative stress caused by both hydrogen peroxide and UV radiation in endometrial adenocarcinoma cells were examined. METHODS: The viability of endometrial adenocarcinoma cells cultured in vitro and treated with different concentrations of quercetin and sodium selenite was measured using the MTT assay. Malondialdehyde (MDA) levels were investigated, and expression levels of BAD and p53 genes were analysed using real-time quantitative polymerase chain reaction. Acridine orange/ethidium bromide staining technique was applied to detect apoptosis. Mass attenuation coeffi cient of each quercetin and sodium selenite combinations was evaluated using Monte Carlo simulation. RESULTS: The combination of quercetin and sodium selenite enhanced cell viability, and reduced MDA levels. The expression levels of BAD and p53 genes decreased by combined treatment with quercetin and selenium while showing synergistic effects in terms of gene expression. Fluorescent microscopic examination showed a decrease in apoptotic cells in endometrial adenocarcinoma cells treated with the combination of quercetin and selenium. CONCLUSIONS: For the fi rst time, selenium and quercetin have synergistic cytoprotective and radioprotective effects on oxidative stress caused by hydrogen peroxide in endometrial adenocarcinoma cells for the fi rst time

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“…Catal et al [ 244 ] used the same injury model using D-GaIN to test the effects of antioxidant supplementation (100 mg/kg/day ascorbic acid +100 mg/kg/day α-tocopherol + 15 mg/kg/day β-carotene + 0.2 mg/kg/day sodium selenite) in the liver. The study described four groups: control, control + antioxidants, D-GaIN and D-GaIN + antioxidants.…”

Section: Third Issue: Bioavailability Of Natural Antioxidants and The...mentioning

confidence: 99%

Natural Antioxidant Evaluation: A Review of Detection Methods

et al. 2022

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Antioxidants have drawn the attention of the scientific community due to being related to the prevention of various degenerative diseases. The antioxidant capacity has been extensively studied in vitro, and different methods have been used to assess its activity. However, the main issues related to studying natural antioxidants are evaluating whether these antioxidants demonstrate a key role in the biological system and assessing their bioavailability in the organism. The majority of outcomes in the literature are controversial due to a lack of method standardization and their proper application. Therefore, this study aims to compile the main issues concerning the natural antioxidant field of study, comparing the most common in vitro methods to evaluate the antioxidant activity of natural compounds, demonstrating the antioxidant activity in biological systems and the role of the main antioxidant enzymes of redox cellular signaling and explaining how the bioavailability of bioactive compounds is evaluated in animal models and human clinical trials.

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An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine- Induced Hepatotoxicity in Rats

Cited by 6 publications

References 45 publications

The effects of vitamins and selenium mixture against brain tissue induced byd‐galactosamine

The effects of vitamins and selenium mixture against brain tissue induced byd‐galactosamine

Synergistic effects of quercetin and selenium on oxidative stress in endometrial adenocarcinoma cells

Natural Antioxidant Evaluation: A Review of Detection Methods

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