An Antinuclear Factor Specific for Leucocytes

Faber, Viggo; P, Elling; Norup, G.; Mansa, B.; Nissen, Nis I.

doi:10.1016/s0140-6736(64)90283-1

Cited by 52 publications

(8 citation statements)

References 7 publications

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“…Although autoantibodies reacting selectively with neutrophil granulocytes have been known for some time to exist in sera from patients with I2.4 [ 1,2], their specificities have remained elusive, and conflicting results have been reported [12-201. In the present study it was shown by I F microscopy on ethanol-fixed leucocytes that almost half of the RA patient sera reacted specifically with neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Specificities of anti-neutrophil autoantibodies in patients with rheumatoid arthritis (RA)

Brimnes

Halberg

Jacobsen

et al. 1997

Clinical and Experimental Immunology

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SUMMARYThe objective of this study was to characterize antigens recognized by neutrophil-specific autoantibodies from patients with RA. Sera from 62 RA patients were screened by indirect immunofluorescence (IIF). Positive sera were further tested by ELISAs for antibodies against various granule proteins and by immunoblotting of electrophoretically separated cell, granule or nuclear extracts. Forty-two sera (68%) reacted with ethanol-fixed neutrophils. In the ELISAs 32% of the 28 medium to strongly IIF-positive sera were negative, while 43% were weakly positive for more than one antigen. Immunoblots of whole neutrophils showed IgG reactions at 25-35 kD, in the 55-kD region, at 80 kD, and at 110kD. Most sera reacted with more than one band. Except for the 55-kD antigen, none of the antigens appeared in lymphocytes. The most notable reactivity in subcellular fractions was with lactofemn and with bands of 25-35 kD from nuclei. In conclusion, anti-neutrophil autoantibodies from RA patients recognize different antigens in the cytoplasm and in the nucleus. Lactofemn is one of the common antigens recognized, but also unknown nuclear antigens of 25-35 kD mol. wt are involved.

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Section: Discussionmentioning

confidence: 99%

Specificities of anti-neutrophil autoantibodies in patients with rheumatoid arthritis (RA)

Brimnes

Halberg

Jacobsen

et al. 1997

Clinical and Experimental Immunology

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“…on tissue sections also failed to react with white-blood-cell nuclei and therefore did not contain leucocyte-specific A.N.F. which has been found in occasional cases of rheumatoid arthritis and Felty's syndrome (Faber et al 1964).…”

Section: Correlation Of Antibodies With Serum Proteins and Clinical Smentioning

confidence: 91%

Auto-antibody Studies in Interstitial Pulmonary Fibrosis

Turner-Warwick¹,

Doniach²

1965

BMJ

117

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Treatment o Dlines-MEDICAL-JiiUNAl also seem that by the end of approximately 12 weeks onequarter (27%) of women had responded to the placebo alone, that a further seventh had responded to imipramine (41 %), and a further fifth to E.C.T. (61%).We have not been able to identify any factors associated with the specific therapeutic responses ; evaluation of demographic, social, and clinical characteristics revealed disappointingly little basis as reliable prognostic indicators. Thus there was no association between outcome as measured by final discharge from hospital and age, history of previous affective illness, and type of onset, previous duration, and clinical severity of the treated illness. Summary and ConclusionsIn 250 patients aged 40-69 years (81 men and 169 women) a comparison has been made between different treatments of depressive illness as specifically defined for the purpose of the trial. The treatments employed were E.C.T., imipramine, a monoamine oxidase inhibitor (phenelzine), and, for a short period of time, a placebo. These treatments were randomly allotted, and comparable groups thus set up and their progress *assessed for six months. Many changes of treatment were made during the later stages of the trial period.On both a short-term basis (after four weeks' treatment in hospital) and on a long-term basis (up to six months) it appears that E.C.T. and imipramine increased the frequency of recovery over and above the spontaneous rate shown by patients on the placebo. This drug, it appears, was specially effective in men and E.C.T. specially effective in women. For these patients, as defined by clinical condition and age, phenelzine revealed no advantage over the placebo in the treatment of men and gave even less favourable results than the placebo in women. Imipramine showed a slower action than E.C.T., but its use certainly reduced the total number of patients for whom E.C.T. was finally regarded as necessary.The Committee is greatly indebted to the 55 physicians (see below) who admitted patients to this trial and completed the records so carefully; to William R.

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“…A common recurrence of granulocytopenia after splenectomy suggests, however, that this is not the only mechanism involved in the development of this disorder, and indicates that extrasplenic factors must be involved (Ruderman, Miller, and Pinals, 1968). In some patients white blood cell antinuclear factors have been shown (Faber, Elling, Norup, Mansa, and Nissen, 1964;Barnes, Turnbull, and VernonRoberts, 1971) Robinson. and form colonies of granulocytes and macrophages in a semisolid tissue culture medium in the presence of an appropriate stimulus (Pluznik and Sachs, 1965;Bradley and Metcalf, 1966). This system has been used extensively to study factors affecting and regulating granulocyte maturation and production.…”

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confidence: 99%