An antimicrobial compound from the endophytic fungus Phoma sp. isolated from the medicinal plant Taraxacum mongolicum

Zhang, Huiru; Xiong, Yuchun; Zhao, Hongyu; Yi, Yanjie; Zhang, Caiyun; Yu, Cuiping; Xu, Chunping

doi:10.1016/j.jtice.2012.11.013

Cited by 20 publications

(13 citation statements)

References 12 publications

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“…Shukla et al (2014) also demonstrated antimicrobial potential of this endophyte against S. aureus (18.3 ± 0.10 mm). Similar results were obtained by Zhang et al (2013a) in a study of fungi associated with Taraxacum mongolicum, and the authors also found positive results against S. aureus (40 mm). Shen et al (2012) has isolated endophytes from branches of Phyllostachys edulis and tested P. herbarum extracts against bacteria; it was not able to inhibit S. aureus and B. subtilis.…”

Section: Discussionsupporting

confidence: 86%

“…Researchers have reported that Phoma species can be isolated from many plants, such as Laguncularia racemosa (Costa et al, 2012), Amaranthus cruentus (Pusz et al, 2015), Taraxacum mongolicum (Zhang et al, 2013a) and Mitrajyna javanica (Pharamat et al, 2013). An important taxonomic paper from CBS-KNAW (The Netherlands) highlights the importance of a polyphasic approach to characterise Phoma species and similar genera, and it presented an overview of the phytopathological importance of this genus (Aveskamp, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Silva¹,

Silvino²,

Bezerra³

et al. 2017

Afr. J. Microbiol. Res.

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The discovery of new metabolites potentially bioactive against pathogenic microorganisms, mainly multidrug resistant, has aroused interest in endophytic fungi. The plant-associated microorganisms have been an important source for development of new compounds of biotechnological interest. This study aimed to investigate the antibacterial capacity of the endophytic fungus, Phoma sp. URM 7221 isolated from the medicinal plant Schinus terebinthifolius against human-pathogenic bacteria. An endophyte was isolated from S. terebinthifolius leaves. Phoma herbarum URM7221 was characterized morphologically and on the basis of ITS rDNA sequence. Primary antimicrobial activity was evaluated using the agar diffusion method and fermentation in liquid medium. Six different solvents were used to extract the active metabolites from fungal biomass and metabolic liquid. An antimicrobial activity test from the extract was carried out using a disk diffusion method with the endophytic extract containing the best antibacterial activity. Two tests were performed: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Partially purified secondary metabolite extracts were analysed by thin layer chromatography (TLC). Liquid metabolically bioactive compounds extracted with petroleum ether revealed a MIC of 25 and 500 μg.mL −1 against S. aureus and MRSA, respectively. Ether and methanol extracts were assessed by chemical analyses and contained phenolic compounds, triterpenes, steroids, reducing sugars, mono-and sesquiterpenes. The thin layer chromatography assay showed the activity of different antimicrobial compounds produced by Phoma sp. URM7221. This endophyte (URM7221) could be efficiently used for production of bioactive metabolites against pathogenic microorganisms, with significant biotechnological potential.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Silva¹,

Silvino²,

Bezerra³

et al. 2017

Afr. J. Microbiol. Res.

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“…A study on the antibacterial activity of an endophytic fungi, Phoma sp. against P. multocida is reported to show an inhibition zone of 26.5 mm (Zhang et al, 2012). The present findings reveal G. magna showing significant antibacterial activity against P. multocida with an inhibition zone of 24 mm.…”

Section: Discussionsupporting

confidence: 52%

Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida Capsular Type A strain

Nath

Joshi

2016

RBT

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Pasteurella multocida is an important veterinary pathogen causing infections in animals and birds. Nowadays, different reports have described the severity of infections, increasing resistance of micro-organisms to antibiotics, and the contribution of ethnoveterinary practices towards the treatment of various ailments of animals. The aim of the present study was to investigate the antibacterial efficacy of the ethanolic extracts of endophytic fungi against P. multocida Capsular Type A strains. A total of six endophytic fungi were isolated from two tropical ethnoveterinary plants: Garcinia xanthochymus H. and Polygonum chinense L. The ethanolic extracts of the endophytic fungi were subjected to in vitro antimicrobial activity by the well diffusion method. Besides, we evaluated the treatment of mice with the potent fungal extract and observed the effects in different organs under electron microscopy. Our results showed that four fungi had antimicrobial activity against the selected pathogen. The best antibacterial activity was showed by the extract of the endophytic fungi, Glomerella magna isolated from G. xanthochymus, with a minimum inhibitory concentration of 46.9 µg/mL and minimum bactericidal concentration of 750 µg/mL. Treatment of mice with the potent fungal extract caused a considerable inhibitory effect on the pathogen growth in vital organs, results that was also confirmed by histopathological studies made by scanning electron microscopy. The present findings indicated that the endophytic fungi G. magna has the potential to provide an effective treatment against infections caused by Pasteurella multocida. However, the isolation of bioactive components needs further investigation. Rev. Biol. Trop. 64 (2): 733-745. Epub 2016 June 01.

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“…The UV (MeOH) k max 243 and 307 nm and 1 H and 13 C NMR data allowed the identification of 6-methyl salicylic acid from Peltaster fructicola, which showed low antifungal activity against Diplodia seriata (Botryosphaeria obtusa) (Venkatasubbaiah et al, 1995;Zhang et al, 2013), and from A. terreus (Fujii et al, 1996). 6-Methyl salicylic acid has been reported to mimic salicylic acid in tobacco by enhancing the accumulation of pathogenesis-related (PR) proteins PR1 and b-1,3-glucanase (Nasser et al, 2001).…”

Section: Extraction Purification and Identification Of Phytotoxinsmentioning

confidence: 99%

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

et al. 2015

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Liquid chromatography-diode array screening of the organic extract of the cultures of 13 isolates of the fungus Neofusicoccum parvum, the main causal agent of botryosphaeria dieback of grapevine, showed similar metabolites. One strain was selected for further chemical studies and led to the isolation and characterisation of 13 metabolites. Structures were elucidated through spectroscopic analyses, including one-and two-dimensional NMR and mass spectrometry, and through comparison to literature data. The isolated compounds belong to four different chemical families: five metabolites, namely, (À)-terremutin (1), (+)-terremutin hydrate (2), (+)-epi-sphaeropsidone (3) (À)-4-chloro-terremutin hydrate (4) and(+)-4-hydroxysuccinate-terremutin hydrate (5), belong to the family of dihydrotoluquinones; two metabolites, namely, (6S,7R) asperlin (6) (11), belong to the family of dihydroisocoumarins; and two of the metabolites, namely, 6-methyl-salicylic acid (12) and 2-hydroxypropyl salicylic acid (13), belong to the family of hydroxybenzoic acids. We determined the phytotoxic activity of the isolated metabolites through a leaf disc assay and the expression of defence-related genes in Vitis vinifera cells cv. Chardonnay cultured with (À)-terremutin (1), the most abundant metabolite. Finally, analysis of the brown stripes of grapevine wood from plants showing botryosphaeria dieback symptoms revealed the presence of two of the isolated phytotoxins. and (6R,7S)-dia-asperlin (7), belong to the family of epoxylactones; four metabolites, namely, (R)-(À)-mellein (8), (3R,4R)-4-hydroxymellein (9), (3R,4S)-4-hydroxymellein (10) (R)(À)-3-hydroxymellein

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An antimicrobial compound from the endophytic fungus Phoma sp. isolated from the medicinal plant Taraxacum mongolicum

Cited by 20 publications

References 12 publications

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Endophytic fungi from tropical ethnoveterinary plants and their antibacterial efficacy against Pasteurella multocida Capsular Type A strain

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

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