An antimicrobial alkaloid and other metabolites produced by Penicillium sp. An endophytic fungus isolated from Mauritia flexuosa L. f.

Abstract

The alkaloid glandicoline B (1) and six other compounds: ergosterol (2), brassicasterol (3), ergosterol peroxide (4), cerevisterol (5), mannitol (6) and 1-O-α-D-glucopyranoside (7) were isolated from Penicillium sp. strain PBR.2.2.2, a fungus from Mauritia flexuosa roots. The structures of the isolated metabolites were established by spectral analysis. MeOH extract of the fungal mycelium at 500 µg mL-1 exhibited antimicrobial activity against Staphylococcus aureus and the compound 1 at 100 µg mL-1 was active against S. aureus, Micrococcus luteus and Escherichia coli. The relationship between the bioactive properties of the fungus PBR.2.2.2 and those achieved for glandicoline B, as well the potential of this substance as bactericide is discussed

Antifungal Polyketides and Other Compounds from Amazonian Endophytic Talaromyces Fungi

“…11,15 The evaluation of the antimicrobial potential of pure compounds was assessed by the microbroth dilution method as recommended by the US National Committee for Clinical Laboratory Standards (NCCLS). 11 The inhibition halo (mm) for extracts and the minimal inhibitory concentrations (MICs, μg mL (Table 3) were used as antibacterial and antifungal positive controls, respectively. Dimethyl sulfoxide (DMSO) 10% was used as negative control.…”

“…10 Our research group has been interested in the chemistry of Amazonian endophytic fungi due to their abilities to produce metabolites with great interest in their ecological associations. 11 In our continuing search for new substances, this study describes the chemical investigation of extracts produced by three endophytic strains belonging to the genus Talaromyces isolated from the roots of Duguetia stelechantha (strain DgCr2 2.1b), root barks of Rollinia mucosa (strain AnspCr1 1.1), and the trabecular structure of Victoria amazonica (strain VrTrb2 1.1). Vol.…”

Biological evaluation and quantitative analysis of antioxidant compounds in pulps of the Amazonian fruits bacuri (Platonia insignisMart.), ingá (Inga edulisMart.), and uchi (Sacoglottis uchiHuber) by UHPLC-ESI-MS/MS

“…Minimal inhibitory concentrations (MICs) were determined by the microbroth‐dilution assay, as recommended by the U.S. National Committee for Clinical Laboratory Standards (NCCLS). Assays were performed into 96‐well plates supplied with 100 μL of Mueller Hinton Broth culture medium, 100 μL of each sample solution, and 5 μL of test bacterial suspensions at 1.0 × 10 7 UFC/mL (Koolen, Soares, Silva, Almeida, & Souza, ). The pulp extracts were dissolved in DMSO at initial concentration of 500 μg/mL being subsequently diluted until 3.75 μg/mL.…”

Triazaspirocycles: Occurrence, Synthesis, and Applications

“…Glandicoline B, first isolated from cultures of P. glandicola [29], has been shown to exhibit antimicrobial activity against Staphyloccoccus aureus , Micrococcus luteus and Escherichia coli at 100 μg/mL [30]. Meleagrin has demonstrated suppression of bacterial fatty acid synthesis through inhibition of enoyl-ACP reductase isoform FabI [11] as well as anti-settlement activity against the barnacle cyprid Banalus amphitrite through inhibition of its molting cycle and attachment mediated by adhesive plaque matrix protein (APMP) [10].…”