An Antifungal Chitinase Produced by Bacillus cereus with Shrimp and Crab Shell Powder as a Carbon Source

Chang, Wei‐Der; Chen, Chin‐Shuh; Wang, San-Lang

doi:10.1007/s00284-002-3955-7

Cited by 86 publications

(62 citation statements)

References 30 publications

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“…The mobile phase-acetonitrile: water (70:30 v/v) at the flow rate of 1 mL/min and detected at 210 nm with NH 2 P 50 -4E column. The retention times for the peaks detected in the crude samples of hydrolytic products were compared (CHANG et al, 2003).…”

Section: Hplc Analysismentioning

confidence: 99%

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Devi¹,

Srinivasan²,

Archana³

et al. 2015

Biosci. J.

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ABSTRACT:The current work was designed to isolate and characterize chitin degrading bacteria. Among the 55 bacterial colonies isolated from 7 different soil samples, 4 isolates were capable of degrading chitinase, among which one strain VITSD3 was found to be potent. Based on the morphological, biochemical and molecular characterization of VITSD3 the isolate was confirmed as Bacillus cereus (Genbank accession number: KC961638), designated as Bacillus cereus VITSD3. The crude enzyme had a total activity of 220 U, precipitated with 44.8 U and 22.5 U for dialysed sample. The hydrolysed product NAG (N-Acetyl Glucosamine) from chitin was analysed by high-pressure liquid chromatography (HPLC).The molecular weight of the chitinase was determined using SDS PAGE and found to be 55 kDa. The partially purified chitinase produced from Bacillus cereus VITSD3 showed antifungal activity against Aspergillus fumigatus (18 mm), Aspergillus niger (6 mm) and Aspergillus flavus (15 mm). Hence the investigation suggests a potential benefit of partially purified chitinase extracted from Bacillus cereus VITSD3 which will serve as an excellent antifungal potential with therapeutic use.

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Section: Hplc Analysismentioning

confidence: 99%

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Devi¹,

Srinivasan²,

Archana³

et al. 2015

Biosci. J.

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“…Most bacterial chitinases, including most of the Gram negative bacteria and Bacillus species belong to family 18. Gram positive bacterial strains, Bacillus thuringiensis and B. cereus, of the family 18 chitinases were shown to produce an important extracellular enzyme in the biocontrol of plant fungal diseases (Chang et al, 2003;Li et al, 2008;Reyes-Ramirez et al, 2004). One recent study indicated that B. lichenformis N1 from the family 18 chitinase exhibited the specific inhibition of fungal spore germination (Lee et al, 2009).…”

mentioning

confidence: 99%

“…Chitinolytic enzymes produced by bacteria and fungi have been postulated to play an important role in the biocontrol of fungal pathogens (Chang et al, 2003;Kobayshi et al, 2002;Lee et al,, 2009;Lorito et al, 1993;ReyesRamirez et al, 2004). Chitinases are divided into two different families (18 and 19) based on amino acid sequence similarities (Henrissat and Davies, 1997).…”

mentioning

confidence: 99%

“…It has been proposed that extracellular chitinase is involved in the biological control of plant pathogens by biocontrol bacterial species of the genera of Bacillus (Chang et al, 2003;Lee et al, 2009;Reyes-Ramirez et al, 2004) and the fungal species of the genus Trichoderma (Lorito et al, 1993). Chitinase production by Serratia plymuthica was not found to be important for its biocontol (Kamensky et al, 2003), but in vitro antifungal activities against Rhizoctonia solani of Chromobacterium sp.…”

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confidence: 99%

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Insight Into Genes Involved in the Production of Extracellular Chitinase in a Biocontrol Bacterium Lysobacter enzymogenes C-3

Choi¹,

Kim²,

Lee³

et al. 2012

The Plant Pathology Journal

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The chitinase producing Lysobacter enzymogenes C-3 has previously been shown to suppress plant pathogens in vitro and in the field, but little is known of the regulation of chitinase production, or its role in antimicrobial activity and biocontrol. In this study, we isolated and characterized chitinase-defective mutants by screening the transposon mutants of L. enzymogenes C-3. These mutations disrupted genes involved in diverse functions: glucose-galactose transpoter (gluP), disulfide bond formation protein B (dsbB), Clp protease (clp), and polyamine synthase (speD). The chitinase production of the SpeD mutant was restored by the addition of exogenous spermidine or spermine to the bacterial cultures. The speD and clp mutants lost in vitro antifungal activities against plant fungal pathogens. However, the gluP and dsbB mutants showed similar antifungal activities to that of the wild-type. The growth of the mutants in nutrient rich conditions containing chitin was similar with that of the wild-type. However, growth of the speD and gluP mutants was defective in chitin minimal medium, but was observed no growth retardation in the clp and dsbB mutant on chitin minimal medium. In this study, we identified the four genes might be involved and play different role in the production of extracellular chitinase and antifungal activity in L. enzymogenes C-3.

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“…Bacterial chitinase activity has often been reported to be responsible for antifungal properties (Chernin et al, 1995, Kobayashi et al, 2002, Chang et al, 2003, Dahiya et al, 2005. However, mutants in the chitinase loci of C. fungivorans Ter331 showed no difference during in vitro antagonism test .…”

Section: Chitinasesmentioning

confidence: 92%

Volatile communication between fungi and bacteria

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An Antifungal Chitinase Produced by Bacillus cereus with Shrimp and Crab Shell Powder as a Carbon Source

Cited by 86 publications

References 30 publications

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

Insight Into Genes Involved in the Production of Extracellular Chitinase in a Biocontrol Bacterium Lysobacter enzymogenes C-3

Volatile communication between fungi and bacteria

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