An Antibody-Immobilized Silica Inverse Opal Nanostructure for Label-Free Optical Biosensors

Lee, Wang Sik; Kang, Taejoon; Kim, Shin‐Hyun; Jeong, Jinyoung

doi:10.3390/s18010307

Cited by 54 publications

(37 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In 2018, SiO 2 -inverse opal (SiO 2 -IO) based biosensor was prepared that showed high sensitivity of 10 3 -10 5 plaque forming unit (PFU), αHA specific antibodies was immobilized on the surface of SiO 2 -IO by using 3-aminopropyl trimethoxysilane (APTMS) that exposes amine group, and a NHS-PEG 4 -maleimide linker was used for binding of APTMS with thiolated protein G (Cys-ProG). HA specific antibodies were attached with the same procedure with protein G. This biosensor is specific and sensitive but virus binding and analysis takes more than 2 h that makes it less preferable [84]. Thus, a number of studies are still going on based on impediametric study and to achieve good sensitivity and specificity based on different modifications of surface of electrodes.…”

Section: Impediametric Biosensormentioning

confidence: 99%

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

et al. 2020

View full text Add to dashboard Cite

H1N1 (Swine flu) is caused by influenza A virus, which is a member of Orthomyxoviridae family. Transmission of H1N1 occurs from human to human through air or sometimes from pigs to humans. The influenza virus has different RNA segments, which can reassert to make new virus strain with the possibility to create an outbreak in unimmunized people. Gene reassortment is a process through which new strains are emerging in pigs, as it has specific receptors for both human influenza and avian influenza viruses. H1N1 binds specifically with an α-2,6 glycosidic bond, which is present in human respiratory tract cells as well as in pigs. Considering the fact of fast multiplication of viruses inside the living cells, rapid detection methods need an hour. Currently, WHO recommended methods for the detection of swine flu include real-time PCR in specific testing centres that take 3–4 h. More recently, a number of methods such as Antigen–Antibody or RT-LAMP and DNA biosensors have also been developed that are rapid and more sensitive. This review describes the various challenges in the diagnosis of H1N1, and merits and demerits of conventional vis-à-vis latest methods with special emphasis on biosensors.

show abstract

Section: Impediametric Biosensormentioning

confidence: 99%

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

et al. 2020

View full text Add to dashboard Cite

show abstract

“…These methods typically show a sensitivity around 10 3 –10 4 TCID 50 mL −1 of virus concentration . Recently, innovative sensing strategies have been developed by using nano‐ and microstructures, contributing to the sensitive and accurate diagnosis of influenza viruses . However, many immunosensing approaches still require specific antibody immobilization steps, depending on the types of materials and structures.…”

Section: Resultsmentioning

confidence: 99%

Surface‐Independent and Oriented Immobilization of Antibody via One‐Step Polydopamine/Protein G Coating: Application to Influenza Virus Immunoassay

Moon

Byun

Kim

et al. 2019

Macromolecular Bioscience

Self Cite

View full text Add to dashboard Cite

For the construction of high‐performance biosensor, it is important to interface bioreceptors with the sensor surface densely and in the optimal orientation. Herein, a simple surface modification method that can optimally immobilize antibodies onto various kinds of surfaces is reported. For the surface modification, a mixture of polydopamine (PDA) and protein G was employed. PDA is a representative mussel‐inspired polymer, and protein G is an immunoglobulin‐binding protein that enables an antibody to have an optimal orientation. The surface characteristics of PDA/Protein G mixture‐coated substrates are analyzed and the PDA/protein G ratio is optimized to maximize the antibody binding efficiency. Moreover, the antibody‐immobilized substrates are applied to the detection of influenza viruses with the naked eye, providing a detection limit of 2.9 × 103 pfu mL‐1. Importantly, the several substrates (glass, SiO2, Si, Al2O3, polyethylene terephthalate, polyethylene, polypropylene, and paper) can be modified by simple incubation with the mixture of PDA/protein G, and then the anti‐influenza A H1N1 antibodies can be immobilized on the substrates successfully. Regardless of the substrate, the influenza viruses are detectable after the sandwich immunoreaction and silver enhancement procedure. It is anticipated that the developed PDA/protein G coating method will extend the range of applicable materials for biosensing.

show abstract

“…Inverse opal structures have been widely used for the implementation of PhC biological sensors, too. As an example, an antibody-immobilized silica-based inverse opal nanostructure was successfully used to label-free detect influenza viruses, with a selectivity for H 1 N 1 subtype and a sensitivity in the range of 10 3 -10 5 plaque forming unit (PFU) [83]. Both the selectivity and the sensitivity have been determined through the measurements of the red shift of the reflectance peak (see Figure 10).…”

Section: Photonic Crystals For Biological Sensingmentioning

confidence: 99%

Photonic Crystal Stimuli-Responsive Chromatic Sensors: A Short Review

et al. 2020

View full text Add to dashboard Cite

Photonic crystals (PhC) are spatially ordered structures with lattice parameters comparable to the wavelength of propagating light. Their geometrical and refractive index features lead to an energy band structure for photons, which may allow or forbid the propagation of electromagnetic waves in a limited frequency range. These unique properties have attracted much attention for both theoretical and applied research. Devices such as high-reflection omnidirectional mirrors, low-loss waveguides, and high- and low-reflection coatings have been demonstrated, and several application areas have been explored, from optical communications and color displays to energy harvest and sensors. In this latter area, photonic crystal fibers (PCF) have proven to be very suitable for the development of highly performing sensors, but one-dimensional (1D), two-dimensional (2D) and three-dimensional (3D) PhCs have been successfully employed, too. The working principle of most PhC sensors is based on the fact that any physical phenomenon which affects the periodicity and the refractive index of the PhC structure induces changes in the intensity and spectral characteristics of the reflected, transmitted or diffracted light; thus, optical measurements allow one to sense, for instance, temperature, pressure, strain, chemical parameters, like pH and ionic strength, and the presence of chemical or biological elements. In the present article, after a brief general introduction, we present a review of the state of the art of PhC sensors, with particular reference to our own results in the field of mechanochromic sensors. We believe that PhC sensors based on changes of structural color and mechanochromic effect are able to provide a promising, technologically simple, low-cost platform for further developing devices and functionalities.

show abstract

An Antibody-Immobilized Silica Inverse Opal Nanostructure for Label-Free Optical Biosensors

Cited by 54 publications

References 49 publications

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

Surface‐Independent and Oriented Immobilization of Antibody via One‐Step Polydopamine/Protein G Coating: Application to Influenza Virus Immunoassay

Photonic Crystal Stimuli-Responsive Chromatic Sensors: A Short Review

Contact Info

Product

Resources

About