An antibody-graphene oxide nanoribbon conjugate as a surface enhanced laser desorption/ionization probe with high sensitivity and selectivity

Abstract

a Graphene oxide nanoribbons (GONRs) were covalently functionalized with an antibody using polyethylene glycol (PEG) as a linker to produce a novel probe for surface enhanced laser desorption/ionization mass spectrometry (SELDI MS). This probe provides a highly sensitive and selective platform for enrichment and MS detection of small molecules in complex media.Detection of biologically important small molecules in complex media by mass spectrometry (MS) is of extreme importance. MS offers high sensitivity, reliability, and outstanding qualitative ability. However, its application is seriously hindered by the ionization suppression effect and matrix interferences. As a result, complicated sample preparation procedures are often required prior to MS analysis. Surface-enhanced laser desorption/ionization (SELDI), a derivant mode of matrix-assisted laser desorption/ionization (MALDI), provides a valuable technique for MS analysis.

Negative ion laser desorption/ionization time‐of‐flight mass spectrometric analysis of small molecules by using nanostructured substrate as matrices

“…Due to the size‐exclusion effect of CMK‐8 against macromolecules, rapid screening, and identification of unknown toxic substances such as perfluorochemicals in human serum can be easily realized. In addition, the same group prepared the antibody‐functionalized graphene oxide nanoribbons (GONRs) by using polyethylene glycol (PEG) as a linker, which was successfully applied as a matrix for selective detection of chloramphenicol in complex sample by negative ion LDI‐TOF MS …”

Covalently Modified Graphenes in Catalysis, Electrocatalysis and Photoresponsive Materials

“…Recently, a poly(fluorine‐perylene‐diimide) covalently to G using in one‐pot strategy has been effectively employed as reusable photocatalyst for the 4‐nitrophenol reduction to 4‐nitroaniline by NaBH 4 under visible light irradiation . An antibody covalently attached to GO through polyethylene glycol as linker resulted in a novel probe for surface enhanced laser desorption/ionization mass spectrometry …”

A layer-by-layer assembled MoS2 thin film as an efficient platform for laser desorption/ionization mass spectrometry analysis of small molecules

“…Many of the promising systems use metal 6 /semiconductor nanoparticles (NPs) 7 and carbon nanomaterials. 8 Use of these nanomaterials overcomes low m/z interferences, but there are several issues that complicate their widespread use, such as the colloidal stability in biological media, 9,10 formation of ‘sweet spots’ by the coffee ring effect 11 and laser-induced decomposition that can lead to the contamination of the mass spectrometer. 12 In this regard, chip-based LDI-MS platforms provide an important alternative to colloidal nanomaterials, with nanostructured silicon, 13 titanium dioxide, 14 layer-by-layer (LBL) assembled films of NPs 15,16 and carbon nanomaterials 17,18 investigated as LDI-MS platforms.…”