An antialgal compound produced by Streptomyces jiujiangensis JXJ 0074T

Zhang, Bing-Huo; Chen, Wei; Li, Han-Quan; Zhou, En‐Min; Hu, Weiyao; Duan, Yan-Qing; Mohamad, Osama Abdalla Abdelshafy; Gao, Rui; Li, Wenjun

doi:10.1007/s00253-015-6584-3

Cited by 25 publications

(18 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, during the pond simulation, one treatment combined the streptomycete isolate (USC-14510) with a sample of cyanobacterium M. aeruginosa and the peak area for geosmin as measured by the GC-MS, was 25% greater than the streptomycete alone. It has been reported that around one quarter of actinomycetes isolated from soil have cyanobacterial lysing properties [39] , [40] , [41] , [42] . The anti-algal compounds produced by certain streptomycetes, such as l -lysine [40] and 2′-deoxyadenosine causes loss of cell integrity, and release of the intracellular contents [41] , [42] .…”

Section: Discussionmentioning

confidence: 99%

Streptophage-mediated control of off-flavour taint producing streptomycetes isolated from barramundi ponds

Jonns

Brooks

Exley

et al. 2017

Synthetic and Systems Biotechnology

View full text Add to dashboard Cite

Off-flavour taint of aquaculture products is a global issue reducing consumer confidence in the farmed produce as they are taken up via the gills of fish, and deposited in the lipids of the animal. If the fish are not purged, resulting undesirable muddy earthy flavour taint can be tasted by consumers. These undesirable flavour and odour is caused by the terpenoid compounds namely geosmin and 2-methylisoborneol, produced as secondary metabolites by certain bacteria including the cyanobacteria and actinomycetes. Current strategies to remediate the problem rely on treating the symptoms not the cause and involve the use of time consuming purging methods and costly chemicals.Biological control using bacteriophages, specific to the problem causing bacteria, offers a natural alternative to chemical control, which might reduce further complications of copper based algaecides and its subsequent implications on water quality. In an adaptation of such biological control approach streptomycetes isolated from barramundi ponds were tested for their susceptibility to streptophages to understand whether host destruction via phage lysis would subsequently eliminate off-flavour taint productions by these isolates.Following the determination of the streptophage susceptibility of the isolates one of the most odourous streptomycete species (USC-14510) was selected to be tested further using different pond simulations resembling real-life applications. Geosmin was tested as the indicator of off-flavour taint production and as it has been previously reported that the cyanobacteria-actinomycete interactions occurring in ponds result in even greater levels of geosmin and 2-methylisoborneol, the geosmin levels for the isolate in the presence of cyanobacteria and streptophages were also tested. Findings indicated that the highly odourous Streptomyces species (USC-14510) once infected with streptophages, can lose its capacity to produce off-flavour taints. Pond simulation studies also revealed geosmin production was significantly reduced when streptophages were introduced into the pond water where streptomycete species were grown. The bacteriophage control method developed in the presented study might again confirm significant potential for the bacteriophage-mediated remediation strategy to be adapted by the aquaculture industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Streptophage-mediated control of off-flavour taint producing streptomycetes isolated from barramundi ponds

Jonns

Brooks

Exley

et al. 2017

Synthetic and Systems Biotechnology

View full text Add to dashboard Cite

show abstract

“…Zhang et al . 44 demonstrated that 2′-deoxyadenosine produced by Streptomyces jiujiangensis strain JXJ 0074 T led to severe crumpling, collapse, and perforation of M. aeruginosa , and a reduction in chlorophyll content. Bacilysin, isolated from B. amyloliquefaciens FZB42, acts against cell walls and also has significant anti-cyanobacterial effects 5 .…”

Section: Discussionmentioning

confidence: 99%

Bactericidal metabolites from Phellinus noxius HN-1 against Microcystis aeruginosa

Jin¹,

Wang²,

Liu³

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Harmful algal blooms cause serious problems worldwide due to large quantities of cyanotoxins produced by cyanobacteria in eutrophic water. In this study, a new compound named 2-(3, 4-dihydroxy-2-methoxyphenyl)-1, 3-benzodioxole-5-carbaldehyde (Compound 1), together with one known compound, 3, 4-dihydroxybenzalacetone (DBL), was purified from Phellinus noxius HN-1 (CCTCC M 2016242). Compound 1 and DBL displayed activity against the cyanobacteria Microcystis aeruginosa with a half maximal effective concentration of 21 and 5 μg/mL, respectively. Scanning electron and transmission electron microscopic observations showed that the compounds caused serious damage and significant lysis to M. aeruginosa cells. qRT-PCR assay indicated that compound 1 and DBL exposure up-regulated the expression of gene mcyB and down-regulated the expression of genes ftsZ, psbA1, and glmS in M. aeruginosa. This study provides the first evidence of bactericidal activity of a new compound and DBL. In summary, our results suggest that compound 1 and DBL might be developed as naturally-based biocontrol agents.

show abstract

“…Extracellular MC-LR content was measured according to a method described previously (37). Before measurement, the supernatant was concentrated to 1/10 of its original volume by freeze-drying and filtered through a 0.45-m filter.…”

Section: Cyanobacterial Liquid Cultures Eightmentioning

confidence: 99%

“…After three cycles of freezing at Ϫ80°C and thawing at 40°C, cell sediments were extracted with 10 ml of acetone-deionized water (1:1, vol/vol) and freeze-dried. The dried material was then resuspended in 0.5 ml of methanol-deionized water (1:1, vol/vol) and filtered through a 0.45-m filter, and then the filtrate was analyzed for intracellular MC-LR by HPLC as described previously (37).…”

Section: Cyanobacterial Liquid Cultures Eightmentioning

confidence: 99%

“…CyanoHAB strains (FACHB-245, FACHB-252, FACHB-905, FACHB-1092, FACHB-1112, FACHB-1171, FACHB-1284, and FACHB-1285) that are commonly found in CyanoHABs were obtained from the Freshwater Algae Culture Collection at the Institute of Hydrobiology (FACHB collection), Chinese Academy of Sciences (http://algae.ihb.ac.cn/english/Cultrues.aspx). The organisms were cultured in HGZ medium at 25°C with an illumination of 30 to 50 mol photon/m 2 /s in a 12-h light/dark cycle as described previously (37). The culturing media and conditions were the same for all cyanobacterial cultures used in this study unless otherwise noted.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology

Zhang

Ding

et al. 2016

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Copper sulfate (CuSO 4 ) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO 4 to other aquatic species and its long-term persistence in the environment. This study reported the isolation and characterization of two natural algicidal compounds, i.e., tryptamine and tryptoline, from Streptomyces eurocidicus JXJ-0089. At a concentration of 5 g/ml, both compounds showed higher algicidal efficiencies than CuSO 4 on Microcystis sp. FACHB-905 and some other harmful cyanobacterial strains. Tryptamine and tryptoline treatments induced a degradation of chlorophyll and cell walls of cyanobacteria. These two compounds also significantly increased the intracellular oxidant content, i.e., superoxide anion radical (O 2 ؊ ) and malondialdehyde (MDA), but reduced the activity of intracellular reductants, i.e., superoxide dismutase (SOD), of cyanobacteria. Moreover, tryptamine and tryptoline treatments significantly altered the internal and external contents of microcystin-LR (MC-LR), a common cyanotoxin. Like CuSO 4 , tryptamine and tryptoline led to releases of intracellular MC-LR from Microcystis, but with lower rates than CuSO 4 . Tryptamine and tryptoline (5 g/ml) in cyanobacterial cultures were completely degraded within 8 days, while CuSO 4 persisted for months. Overall, our results suggest that tryptamine and tryptoline could potentially serve as more efficient and environmentally friendly alternative algicides than CuSO 4 in controlling harmful cyanobacterial blooms. IMPORTANCECyanobacterial harmful algal blooms (CyanoHABs) in aquatic environments have become a worldwide problem. Numerous efforts have been made to seek means to prevent, control, and mitigate CyanoHABs. Copper sulfate (CuSO 4 ), was once a common algicide to treat and control CyanoHABs. However, its application has become limited due to concerns about its general toxicity to other aquatic species and its long-term persistence in the environment. There is a great need for algicides with higher specificity and low environmental impacts. This study reports the isolation and characterization of two natural algicidal compounds from a streptomycete strain, Streptomyces eurocidicus JXJ-0089. Our results suggest that the identified algicides could potentially serve as more efficient and environmentally friendly alternative algicides than CuSO 4 in controlling harmful cyanobacterial blooms. Harmful cyanobacterial blooms (CyanoHABs) have become a global phenomenon and are occurring with increasing intensity, area of infection, and frequency (1). One danger that CyanoHABs have posed to the environment and human health is the production and release of cyanotoxins. The most frequently detected cyanotoxins in freshwater systems are microcystins (MCs), a group of potent liver toxins (2, 3). An MC contamination incident in 1996 caused the death of 53 patients in Caruaru, Brazil (4). A more recent MC c...

show abstract

An antialgal compound produced by Streptomyces jiujiangensis JXJ 0074T

Cited by 25 publications

References 43 publications

Streptophage-mediated control of off-flavour taint producing streptomycetes isolated from barramundi ponds

Streptophage-mediated control of off-flavour taint producing streptomycetes isolated from barramundi ponds

Bactericidal metabolites from Phellinus noxius HN-1 against Microcystis aeruginosa

Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology

Contact Info

Product

Resources

About