An Anti-okadaic Acid-Anti-idiotypic Antibody Bearing An Internal Image of Okadaic Acid Inhibits Protein Phosphatase PP1 and PP2A Catalytic Activity

Shestowsky, William S.; Holmes, Charles F.B.; Hu, Tingmo; Marr, J. C.; Wright, Jeffrey L. C.; Chin, Jayne; Sikorska, Hanna

doi:10.1006/bbrc.1993.1414

Cited by 18 publications

(9 citation statements)

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“…However, antibody-based methods provide no information about the activity of the analogues detected, and only identify chemicals with the specific structure recognized by the antibody used in the assay. Consequently, some analogs may not be detected (false negatives) or non-toxic compounds can be detected (false positives) [ 331 , 332 , 333 ].…”

Section: Assessment Of Sample Collection Procedures and Available mentioning

confidence: 99%

Dinophysis Toxins: Causative Organisms, Distribution and Fate in Shellfish

et al. 2014

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Several Dinophysis species produce diarrhoetic toxins (okadaic acid and dinophysistoxins) and pectenotoxins, and cause gastointestinal illness, Diarrhetic Shellfish Poisoning (DSP), even at low cell densities (<103 cells·L−1). They are the main threat, in terms of days of harvesting bans, to aquaculture in Northern Japan, Chile, and Europe. Toxicity and toxin profiles are very variable, more between strains than species. The distribution of DSP events mirrors that of shellfish production areas that have implemented toxin regulations, otherwise misinterpreted as bacterial or viral contamination. Field observations and laboratory experiments have shown that most of the toxins produced by Dinophysis are released into the medium, raising questions about the ecological role of extracelular toxins and their potential uptake by shellfish. Shellfish contamination results from a complex balance between food selection, adsorption, species-specific enzymatic transformations, and allometric processes. Highest risk areas are those combining Dinophysis strains with high cell content of okadaates, aquaculture with predominance of mytilids (good accumulators of toxins), and consumers who frequently include mussels in their diet. Regions including pectenotoxins in their regulated phycotoxins will suffer from much longer harvesting bans and from disloyal competition with production areas where these toxins have been deregulated.

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Section: Assessment Of Sample Collection Procedures and Available mentioning

confidence: 99%

Dinophysis Toxins: Causative Organisms, Distribution and Fate in Shellfish

et al. 2014

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“…In addition, variations of the Adda (3-amino-9-methoxy-2,6,8,-trimethyl-10-phenyl-deca-4(E),6(E)-dienoic acid) at position 5 have been described [15]. To date more than 60 MCs have been isolated and characterized [16,17].…”

Section: Introductionmentioning

confidence: 98%

Liquid chromatography-mass spectrometry identification of microcystins inMicrocystis aeruginosa strain from lake Thanh Cong, Hanoi, Vietnam

Hummert¹,

Dahlmann

Reinhardt

et al. 2001

Chromatographia

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“…Within the last few years, numerous anti-id antibodies against small molecular weight haptens, including mycotoxins such as T-2 toxin (Chanh et al, 1989(Chanh et al, , 1990(Chanh et al, , 1992, insecticides, herbicides (Spinks et al, 1993), hormones (Khole & Hegde, 1992;Madhok et al, 1992) and phycotoxins (Shestowsky et al, 1992(Shestowsky et al, , 1993 have been successfully generated. Some anti-id polyclonal antibodies (Ab2) have been shown to mimic effectively the biological functions of the hapten (Nisonoff, 1991).…”

Section: Introductionmentioning

confidence: 99%

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b₁

Hsu

Chu

1995

Food and Agricultural Immunology

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Polyclonal anti-idiotype antibodies pAb2) for aflatoxin were generated from mice ascites after immunization with affinity-purified rabbit polyclonal antibody (pAbl) against aflatoxin B 1 (pAFB 1 ). After affinity chromatography purification, pAb2 were subjected to various analyses, and were used to generate anti-anti-id antibodies (pAb3) in the ascites of Balb/c mice. ELISA analyses revealed that the anti-id antibodies bound specifically to the original pAb1, but not to other types of monoclonal antibodies or normal mouse IgG. The inhibition of binding of AFB 1 to pAb1 by pAb2 was demonstrated in both regular and biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) systems in which AFB 1 -bovine serum albumin (BSA) was coated on to the microtiter plate. The concentrations causing 50% inhibition (ID 50 ) of the binding of pAbl to AFB 1 -BSA by pAb2 were found to be 6.5 and 1.7 µg/assay respectively. Inhibition of the binding of pAb1 to the solid-phase pAb2 by free AFB 1 (ID 50 = 0.63 µ/assay) was also demonstrated in the ELISA. pAb3 were found to have similar characteristics to the original pAb1. In the direct ELISA, the ID 50 of binding of AFB 1 -horseradish peroxidase to the solid-phase pAb3 by AFB 1 was found to be 0.20 ng ml -1 . In the indirect ELISA, the ID 50 of the binding of pAb3 to the solid-phase AFB 1 -BSA by AFB 1 was found to be 1.84 ng ml -1 Analysis of a naturally contaminated corn sample for AFB 1 by the pAb3-based ELISA gave a result similar to that obtained from the regular pAb1-based ELISA (19.7 and 21.8 µg kg -1 respectively).

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An Anti-okadaic Acid-Anti-idiotypic Antibody Bearing An Internal Image of Okadaic Acid Inhibits Protein Phosphatase PP1 and PP2A Catalytic Activity

Cited by 18 publications

References 0 publications

Dinophysis Toxins: Causative Organisms, Distribution and Fate in Shellfish

Dinophysis Toxins: Causative Organisms, Distribution and Fate in Shellfish

Liquid chromatography-mass spectrometry identification of microcystins inMicrocystis aeruginosa strain from lake Thanh Cong, Hanoi, Vietnam

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b₁

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An Anti-okadaic Acid-Anti-idiotypic Antibody Bearing An Internal Image of Okadaic Acid Inhibits Protein Phosphatase PP1 and PP2A Catalytic Activity

Cited by 18 publications

References 0 publications

Dinophysis Toxins: Causative Organisms, Distribution and Fate in Shellfish

Dinophysis Toxins: Causative Organisms, Distribution and Fate in Shellfish

Liquid chromatography-mass spectrometry identification of microcystins inMicrocystis aeruginosa strain from lake Thanh Cong, Hanoi, Vietnam

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b1

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Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b₁