An Anti-mutant Approach for Antimicrobial Use

Drlica, Karl; Wang, J.-Y.; Malik, Muhammad Abdul; Lu, Tao; Park, Steven; Li, Xinying; Perlin, David S.; Zhao, Xilin

doi:10.1007/978-0-387-72418-8_11

Cited by 4 publications

(2 citation statements)

References 153 publications

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“…This binding ultimately halts the synthesis of peptidoglycan, leading to bacterial cell death (Guilfoile and Alcamo, 2007). It is important to note that β-Lactam antibiotics are effective against actively growing bacteria that are in the process of synthesizing their cell walls (Drlica and Fong, 2008). The antimicrobial properties of chitin, chitosan, and their derivatives have gained significant attention in recent years, particularly in their effectiveness against various microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Optimizing Chitosan Derived from White Shrimp (Metapenaeus affinis): A Novel Approach to Inhibit Biofilm Formation and Quorum Sensing in Pseudomonas aeruginosa

Riahi,

Tajbakhsh,

Mabudi

et al. 2024

Preprint

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Pseudomonas aeruginosa is a commonly found Gram-negative bacterium in healthcare facilities and is renowned for its ability to form biofilms and its virulence factors that are controlled by quorum sensing (QS) systems. The increasing prevalence of multidrug-resistant strains of this bacterium poses a significant challenge in the field of medicine. Consequently, the exploration of novel antimicrobial agents has become a top priority. This research aims to optimize chitosan derived from white shrimp (Metapenaeus affinis) using the Response Surface Methodology computational approach. The objective is to investigate chitosan’s potential as a solution for inhibiting QS activity and biofilm formation in P. aeruginosa ATCC 10145. Under optimized conditions, chitin treated (NaOH: 1.41 M) for 15.75 hours, HCl (7.49% vol) for 2.01 hours, at a deacetylation temperature of 81.15 °C. Resulting chitosan exhibited a degree of deacetylation exceeding 93.98%, as confirmed by Fourier-transform infrared spectral analysis, indicating its high purity. The extracted chitosan demonstrated a significant synergistic antibiotic effect against P. aeruginosa when combined with ceftazidime, enhancing its bactericidal activity by up to 15-fold. In addition, sub-MIC concentrations of extracted chitosan (10 and 100 μg/mL) successfully reduced the production of pyocyanin and rhamnolipid, as well as the swimming motility, protease activity and biofilm formation in comparison to the control group. Moreover, chitosan treatment downregulated the RhlR and LasR genes in P. aeruginosa when compared to the control group (P<0.05). The optimized chitosan extract shows significant potential as a coating agent for surgical equipment, effectively preventing nosocomial infections caused by P. aeruginosa pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Optimizing Chitosan Derived from White Shrimp (Metapenaeus affinis): A Novel Approach to Inhibit Biofilm Formation and Quorum Sensing in Pseudomonas aeruginosa

Riahi,

Tajbakhsh,

Mabudi

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…This binding ultimately halts the synthesis of peptidoglycan, leading to bacterial cell death (Guilfoile and Alcamo 2007 ). It is important to note that β-Lactam antibiotics are effective against actively growing bacteria that are in the process of synthesizing their cell walls (Drlica and Fong 2008 ). The antimicrobial properties of chitin, chitosan, and their derivatives have gained significant attention in recent years, particularly in their effectiveness against various microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Optimizing chitosan derived from Metapenaeus affinis: a novel anti-biofilm agent against Pseudomonas aeruginosa

Riahi,

Mabudi,

Tajbakhsh

et al. 2024

AMB Expr

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Pseudomonas aeruginosa is a commonly found Gram-negative bacterium in healthcare facilities and is renowned for its ability to form biofilms and its virulence factors that are controlled by quorum sensing (QS) systems. The increasing prevalence of multidrug-resistant strains of this bacterium poses a significant challenge in the field of medicine. Consequently, the exploration of novel antimicrobial agents has become a top priority. This research aims to optimize chitosan derived from white shrimp (Metapenaeus affinis) using the Response Surface Methodology (RSM) computational approach. The objective is to investigate chitosan’s potential as a solution for inhibiting QS activity and biofilm formation in P. aeruginosa ATCC 10,145. Under optimized conditions, chitin was treated with NaOH (1.41 M) for 15.75 h, HCl (7.49% vol) for 2.01 h, and at a deacetylation temperature of 81.15 °C. The resulting chitosan exhibited a degree of deacetylation (DD%) exceeding 93.98%, as confirmed by Fourier-transform infrared (FTIR) spectral analysis, indicating its high purity. The extracted chitosan demonstrated a significant synergistic antibiotic effect against P. aeruginosa when combined with ceftazidime, enhancing its bactericidal activity by up to 15-fold. In addition, sub-MIC (minimum inhibitory concentration) concentrations of extracted chitosan (10 and 100 µg/mL) successfully reduced the production of pyocyanin and rhamnolipid, as well as the swimming motility, protease activity and biofilm formation ability in comparison to the control group (P < 0.05). Moreover, chitosan treatment downregulated the RhlR and LasR genes in P. aeruginosa when compared to the control group (P < 0.05). The optimized chitosan extract shows significant potential as a coating agent for surgical equipment, effectively preventing nosocomial infections caused by P. aeruginosa pathogens.

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Mutant Selection Window Hypothesis: A Framework for Anti-mutant Dosing of Antimicrobial Agents

Drlica¹,

Zhao²

2008

National Institute of Allergy and Infectious Diseases, NIH

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An Anti-mutant Approach for Antimicrobial Use

Cited by 4 publications

References 153 publications

Optimizing Chitosan Derived from White Shrimp (Metapenaeus affinis): A Novel Approach to Inhibit Biofilm Formation and Quorum Sensing in Pseudomonas aeruginosa

Optimizing Chitosan Derived from White Shrimp (Metapenaeus affinis): A Novel Approach to Inhibit Biofilm Formation and Quorum Sensing in Pseudomonas aeruginosa

Optimizing chitosan derived from Metapenaeus affinis: a novel anti-biofilm agent against Pseudomonas aeruginosa

Mutant Selection Window Hypothesis: A Framework for Anti-mutant Dosing of Antimicrobial Agents

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