An anti-inflammatory lectin from Luetzelburgia auriculata seeds inhibits adhesion and rolling of leukocytes and modulates histamine and PGE2 action in acute inflammation models

Alencar, Nylane Maria Nunes de; Oliveira, Raquel Sombra Basílio de; Figueiredo, J. G.; Cavalcante, Ítalo José Mesquita; Matos, M.; Cunha, Fernando; Nunes, João Victor Serra; Bomfim, Liezelotte R.; Ramos, Márcio V.

doi:10.1007/s00011-009-0092-9

Cited by 20 publications

(16 citation statements)

References 35 publications

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“…This shunting is a possible mechanism for the increased ankle swelling seen in the 5-LO -/- mice in the current study. Due to the genetic deletion of 5-LO, increased free AA may be used by COX-2 to produce higher levels of PGE 2 , which is known to induce edema (53). Substrate shunting between these two enzymes has been described previously using specific COX-2 inhibitors (54) or mice deficient in microsomal PGE 2 synthase (55).…”

Section: Discussionmentioning

confidence: 99%

5-Lipoxygenase–Deficient Mice Infected withBorrelia burgdorferiDevelop Persistent Arthritis

Blaho

Zhang

Hughes-Hanks

et al. 2011

The Journal of Immunology

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The enzyme 5-lipoxygenase (5-LO) catalyzes the conversion of arachidonic acid into the leukotrienes, which are critical regulators of inflammation and inflammatory diseases, such as asthma and arthritis. Although leukotrienes are present in the synovial fluid of Lyme disease patients, their role in the development of Lyme arthritis has not been determined. In the current study, we used a murine model of Lyme arthritis to investigate the role 5-LO products might have in the development of this inflammatory disease. Following infection of Lyme arthritis-susceptible C3H/HeJ mice with B. burgdorferi, mRNA expression of 5-LO and 5-lipoxygenase-activating protein (FLAP) was induced in the joints, and the 5-LO product LTB4 was produced. Utilizing C3H 5-LO-deficient mice, we demonstrated that 5-LO activity was not necessary for the induction of Lyme arthritis, but that its deficiency resulted in earlier joint swelling and an inability to resolve arthritis as demonstrated by sustained arthritis pathology through day 60 post-infection. Although production of anti-Borrelia IgG was decreased in 5-LO-deficient mice, bacterial clearance from the joints was unaffected. Phagocytosis of B. burgdorferi and efferocytosis of apoptotic neutrophils was defective in macrophages from 5-LO-deficient mice, and uptake of opsonized spirochetes by neutrophils was reduced. These results demonstrate that products of the 5-LO metabolic pathway are not required for the development of disease in all models of arthritis, and that caution should be used when targeting 5-LO as therapy for inflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

5-Lipoxygenase–Deficient Mice Infected withBorrelia burgdorferiDevelop Persistent Arthritis

Blaho

Zhang

Hughes-Hanks

et al. 2011

The Journal of Immunology

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“…HGA demonstrated the ability to agglutinate human erythrocytes with preference for type A treated with papain. Previous studies have shown the ability of some lectins to reduce the rolling and adhesion of neutrophils on the endothelium, possibly by blocking interactions of adhesion molecules present on neutrophils (L-selectins) and endothelial cells (Eand P-selectins) [26,27,57,58]. Marine lectins are generally identified by their metal ion requirement for their hemagglutination activity.…”

Section: Discussionmentioning

confidence: 99%

“…A considerable number of marine invertebrate lectins have already been purified and characterized, and considerable attention is focused on its biological recognition role [8][9][10][11][12]. The Table I summarizes the origin, specificity, and biological activity from some lectins [5,[17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35]. Then, they come as ancestral molecules that show a high degree of conservation during the evolution to superior vertebrates [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Holothuria grisea agglutinin (HGA): the first invertebrate lectin with anti‐inflammatory effects

Moura

Aragão

Melo

et al. 2012

Fundamemntal Clinical Pharma

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Holothuria grisea agglutinin (HGA) is a dimeric lectin of molecular mass 228 kDa by gel filtration with monomers of 105 kDa by SDS-PAGE. The lectin is highly thermostable as it retains full activity for 1 h at 70 °C. Unlike other lectins purified from marine invertebrates, the hemagglutination activity of HGA does not require any divalent metal ions. The affinity analysis of HGA showed that only mucin was able to inhibit the hemagglutinating activity. HGA administered intravenously was tested in classical models of nociception and inflammation. HGA was able to inhibit neutrophil migration into the peritoneal cavity induced by carrageenan. This inhibitory effect was 68% at a dose of 1 mg/kg. In acetic acid-induced writhing tests, a significant antinociceptive effect was observed by treatment with HGA (0.1; 1 or 10 mg/kg) reducing constrictions by 27, 90 and 84%, respectively. In formalin tests, HGA at a dose of 10 mg/kg showed antinociceptive effect only in the inflammatory phase (phase 2). Nevertheless, in hot-plate tests, HGA did not show any nociceptive effect. In rota-rod and open-field tests, HGA did not alter the animals' behavior. The treatment with HGA 10 mg/kg presented diminished myeloperoxidase activity activity (81.6% inhibition) and raised the circulating levels of NO by 50.4% when compared with the carrageenan group. HGA has demonstrated the ability to modulate the inflammatory response in models of inflammation in vivo. HGA is the first marine invertebrate lectin that showed an anti-inflammatory effect. This finding opens a new perspective on the potential of lectins from the marine environment.

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“…Most of these studies were performed in rodents via systemic treatment with lectins that displays binding specificity for glucose–mannose (Assreuy et al ., ; Assreuy et al ., ; Rocha et al ., ; Simoes et al ., ; Pinto et al ., ; Silva et al ., ) and N ‐acetylglucosamine (Alencar et al ., ; Alencar et al ., ; Napimoga et al ., ). However, the anti‐inflammatory effect of galactose‐binding lectins has only been demonstrated for those isolated from the leguminous Luetzelburgia auriculata (Alencar et al ., ).…”

Section: Introductionmentioning

confidence: 99%

The galactose‐binding lectin isolated from Bauhinia bauhinioides Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines

Girão

Cavada

Pires

et al. 2015

J of Molecular Recognition

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In this study, the amino acid sequence and anti-inflammatory effect of Bauhinia bauhinioides (BBL) lectin were evaluated. Tandem mass spectrometry revealed that BBL possesses 86 amino acid residues. BBL (1 mg/kg) intravenously injected in rats 30 min prior to inflammatory stimuli inhibited the cellular edema induced by carrageenan in only the second phase (21% - 3 h, 19% - 4 h) and did not alter the osmotic edema induced by dextran. BBL also inhibited carrageenan peritoneal neutrophil migration (51%), leukocyte rolling (58%) and adhesion (68%) and the neutrophil migration induced by TNF-α (64%). These effects were reversed by the association of BBL with galactose, demonstrating that the carbohydrate-binding domain is essential for lectin activity. In addition, BBL reduced myeloperoxidase activity (84%) and TNF-α (68%) and IL1-β (47%) levels. In conclusion, the present investigation demonstrated that BBL contains highly homologous isolectins, resulting in a total of 86 amino acid residues, and exhibits anti-inflammatory activity by inhibiting neutrophil migration by reducing TNF-α and IL1-β levels via the lectin domain.

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An anti-inflammatory lectin from Luetzelburgia auriculata seeds inhibits adhesion and rolling of leukocytes and modulates histamine and PGE2 action in acute inflammation models

Abstract: The results indicate that this lectin is a potent anti-inflammatory molecule. Its effects engage diverse modulatory events.

Cited by 20 publications

References 35 publications

5-Lipoxygenase–Deficient Mice Infected withBorrelia burgdorferiDevelop Persistent Arthritis

5-Lipoxygenase–Deficient Mice Infected withBorrelia burgdorferiDevelop Persistent Arthritis

Holothuria grisea agglutinin (HGA): the first invertebrate lectin with anti‐inflammatory effects

The galactose‐binding lectin isolated from Bauhinia bauhinioides Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines

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