An anti-BSA antibody-based immunochromatographic assay for chloramphenicol and aflatoxin M1 by using carboxy-modified CdSe/ZnS core–shell nanoparticles as label

Qie, Zhiwei; Yan, Wenliang; Gao, Zichen; Wu, Meng; Xiao, Rui; Wang, Shengqi

doi:10.1007/s00604-019-4009-1

Cited by 27 publications

(3 citation statements)

References 36 publications

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“…The inter-assay recoveries ranged from 90 to 120%, with a CV ranging from 8.33 to 11.66%. These results prove that this immunosensor benefits from high accuracy and precision [27]. Since long-term stability studies were not the purpose of our work, we did not check it, but taking into account the several weeks of stability of commercially available pregnancy tests, we can expect similar performance [28].…”

Section: Analytical Performance Of the Developed Smartphone-based Lfia Kitmentioning

confidence: 76%

On-Site Detection of Carcinoembryonic Antigen in Human Serum

et al. 2021

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Real-time connectivity and employment of sustainable materials empowers point-of-care diagnostics with the capability to send clinically relevant data to health care providers even in low-resource settings. In this study, we developed an advantageous kit for the on-site detection of carcinoembryonic antigen (CEA) in human serum. CEA sensing was performed using cellulose-based lateral flow strips, and colorimetric signals were read, processed, and measured using a smartphone-based system. The corresponding immunoreaction was reported by polydopamine-modified gold nanoparticles in order to boost the signal intensity and improve the surface blocking and signal-to-noise relationship, thereby enhancing detection sensitivity when compared with bare gold nanoparticles (up to 20-fold in terms of visual limit of detection). Such lateral flow strips showed a linear range from 0.05 to 50 ng/mL, with a visual limit of detection of 0.05 ng/mL and an assay time of 15 min. Twenty-six clinical samples were also tested using the proposed kit and compared with the gold standard of immunoassays (enzyme linked immunosorbent assay), demonstrating an excellent correlation (R = 0.99). This approach can potentially be utilized for the monitoring of cancer treatment, particularly at locations far from centralized laboratory facilities.

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Section: Analytical Performance Of the Developed Smartphone-based Lfia Kitmentioning

confidence: 76%

On-Site Detection of Carcinoembryonic Antigen in Human Serum

et al. 2021

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“…The optimal amounts of anti-AFM1 antibody and anti-CAP antibody were about 7 μg and 18 μg, respectively, for 2 mL gold nanoparticles. The previous works [ 24 , 25 ] developed a fluorescent-based immunostrip to detect AFM1 or CAP in milk. Their AFM1 and CAP detection limits of their immunostrips were below 0.1 ng/mL, but extra equipment was required to obtain the fluorescent signal, making them impossible to use by the general public.…”

Section: Discussionmentioning

confidence: 99%

A Sensitive Two-Analyte Immunochromatographic Strip for Simultaneously Detecting Aflatoxin M1 and Chloramphenicol in Milk

Liu

et al. 2020

Toxins

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A two-analyte immunochromatographic strip (immunostrip) was developed for the simultaneous detection of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. Protein conjugates (AFM1-ovalbumin (OVA) and CAP-OVA) and goat anti-rabbit IgG were respectively drawn on nitrocellulose membrane as two test lines (T1 and T2) and a control line (C). The immunostrip was dipped into a well that contained a 200 μL milk sample, 5 μL AFM1 antibody-gold conjugates, and 8 μL CAP antibody-gold conjugates; the whole assay was completed in 15 min and the results could be interpreted visually or using a reader. This immunostrip has cut-off levels of 0.1 ng/mL and 0.5 ng/mL for AFM1 and CAP, respectively. Analysis of CAP and AFM1 in milk samples revealed that data from the immunostrip test agreed closely with those obtained from ELISA. The two-analyte immunostrip is a rapid way for on-site simultaneous detection of AFM1 and CAP in milk.

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“…The low sensitivity of the colorimetric readout mode limits the application of conventional LFIA in fields that require highly sensitive and quantitative assays [20]. Instead of gold nanoparticles, various novel nanomaterials, including organic and inorganic dye-doped nanoparticles [21], up-conversion phosphors [22,23], magnetic nanoparticles [24], and fluorescent materials [25,26], have been utilized to improve the sensitivity of LFIA. Among these signal reporters, fluorescence microspheres are most commonly used because of their excellent performance [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products

Han,

Fan,

Liu

et al. 2023

Molecules

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Quantum dot nanobeads (QBs) were used as signal source to develop competitive lateral flow immunoassay (LFIA) for the detection of chloramphenicol (CAP). The quantitative detection of CAP was achieved by calculating the total color difference (∆E) values of the test line (T line) using the images of test strips. QB-based LFIA (QBs-LFIA) allowed the effective dynamic linear detection of CAP in the range of 0.1–1.5 ng/mL. The limit of detection (LOD) was 3.0 ng/mL, which was 50 and 667 times lower than those achieved for two different brands of colloidal gold kits. The recoveries of CAP during real-sample detection were 82.82–104.91% at spiked levels of 0.1, 0.7, and 1.5 ng/mL. These results indicate that the developed QBs-LFIA facilitates the sensitive detection of CAP.

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An anti-BSA antibody-based immunochromatographic assay for chloramphenicol and aflatoxin M1 by using carboxy-modified CdSe/ZnS core–shell nanoparticles as label

Cited by 27 publications

References 36 publications

On-Site Detection of Carcinoembryonic Antigen in Human Serum

On-Site Detection of Carcinoembryonic Antigen in Human Serum

A Sensitive Two-Analyte Immunochromatographic Strip for Simultaneously Detecting Aflatoxin M1 and Chloramphenicol in Milk

Lateral Flow Immunoassay Based on Quantum-Dot Nanobeads for Detection of Chloramphenicol in Aquatic Products

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