An Antennae-Enriched Odorant-Binding Protein EonuOBP43 Mediate the Behavioral Response of the Tea Green Leafhopper, <i>Empoasca onukii</i> Matsuda to the Host and Nonhost Volatiles

Lun, Xiaoyue; Xu, Xiuxiu; Zhang, Yu; Zhang, Ruirui; Cao, Yan; Zhang, Xiangzhi; Jin, Meina; Zhang, Zhengqun; Zhao, Yunhe

doi:10.1021/acs.jafc.3c07144

Cited by 3 publications

(1 citation statement)

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“…Based on the number of conserved cysteine (Cys) residues, insect OBPs can be divided into five subfamilies: classic OBPs (six conserved cysteines), plus-C OBPs (eight conserved cysteines), minus-C OBPs (four conserved cysteines), dimer OBPs (twelve conserved cysteines) and atypical OBPs (nine to ten conserved cysteines) [16,17]. Since the first OBP was found in Antheraea polyphemus [18], more OBPs have been identified based on the transcriptome sequencing technique in recent years [19][20][21][22][23][24][25][26]. Collectively, these studies illustrated that OBPs, which were highly expressed in antennae as well as other body parts (e.g., legs, wings and larval maxilla), showed specific binding affinities with different host plant volatiles, and this phenomenon indicated that OBPs have the function of orientation and host selection at the molecular level.…”

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confidence: 99%

The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein SvelOBP1 from Sympiezomias velatus (Coleoptera: Curculionidae) to Jujube Volatiles

Zhai,

Zhang,

Tian

et al. 2024

Life

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Sympiezomias velatus (Chevrolat) (Coleoptera: Curculionidae) has caused serious damage on jujube trees (Ziziphus jujuba Mill) in northern China. Semiochemicals emerging from the host are essential in the process of insects identifying and localizing the host. The highly expressed odorant-binding protein 1 of S. velatus (SvelOBP1) was assumed to play a possible role in the recognition of host volatiles. In this study, SvelOBP1 was cloned based on the antennal transcriptome of S. velatus. The recombinant SvelOBP1 protein was expressed in Escherichia coli and purified by Ni-NTA resin. The predicted protein SvelOBP1 belonged to a classic OBP subfamily. The expression patterns revealed that SvelOBP1 was mainly expressed in the antennae of both males and females, whereas the expression of SvelOBP1 in other body parts could be neglected. The fluorescence binding assay indicated that SvelOBP1 displayed very strong binding affinities to dibutyl benzene-1,2-dicarboxylate and (Z)-hex-3-en-1-ol (Ki = 6.66 ± 0.03 and 7.98 ± 0.06 μM). The molecular docking results showed that residues Trp114, Phe115 and Asp110 may be involved in binding to both dibutyl benzene-1,2-dicarboxylate and (Z)-hex-3-en-1-ol and may have a great impact on odorant recognition of S. velatus. Our results provide evidence that SvelOBP1 might participate in the olfactory molecular perception of S. velatus and would promote the development of pest attractants for S. velatus control.

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Section: Introductionmentioning

confidence: 99%

The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein SvelOBP1 from Sympiezomias velatus (Coleoptera: Curculionidae) to Jujube Volatiles

Zhai,

Zhang,

Tian

et al. 2024

Life

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Construction of a comprehensive evaluation model for constitutive resistance traits of tea cultivars to the pests Matsumurasca onukii and Dendrothrips minowai

Sun,

Chen,

Jin

et al. 2024

Preprint

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As major pests in tea plantations, Matsumurasca onukii Matsuda (Hemiptera: Cicadellidae) and Dendrothrips minowai Priesner (Thysanoptera: Thripidae) reduce tea yields and cause large economic loss. Host plant resistance is one of the most effective and economical potential pest management strategies but is not well understood in tea. This study aimed to screen tea lines to determine chemical and structural traits that were associated with resistance to both herbivore species and then develop comprehensive resistance indicators and evaluation model of insect resistance. In addition, we modelled host plant identification and selection by the two pests and established resistance grading criteria for each. Traits positively associated with resistance to M. onukii were: concentrations of nonanal and dodecane, epidermal thickness of adaxial leaf surface, and ratio of this to leaf thickness. Negatively associated traits were: concentrations of malonic dialdehyde and linalool, ratio of adaxial leaf cuticle thickness to leaf thickness, and ratio of abaxial cuticle thickness to leaf thickness. For D. minowai, length of leaf, trichome density of buds, and caffeine content were positively associated with resistance; whilst dodecane and phenethyl alcohol concentrations as well as several color parameters of foliage were negatively associated. To comprehensively evaluate the insect resistance of tea cultivars, the affiliation function method was used and the results of the model showed significantly correlation for observed population densities of both pests. This study provides the first comprehensive framework for host plant resistance traits and will underpin risk assessment among existing cultivars and selection in future plant breeding programs.

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Mixture of Synthetic Plant Volatiles Attracts More Stick Tea Thrips Dendrothrips minowai Priesner (Thysanoptera: Thripidae) and the Application as an Attractant in Tea Plantations

Xu,

Zhang,

Qiu

et al. 2024

Plants

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The stick tea thrip (Dendrothrips minowai) is one of the most serious sucking pests of tea plants (Camellia sinensis) in China, North Korea, and Japan. Plant volatile lures are widely used for both monitoring and mass trapping. Previously, we demonstrated that sticky traps baited with p-anisaldehyde, eugenol, farnesene, or 3-methyl butanal captured significantly more D. minowai in tea plantations, with p-anisaldehyde notably capturing the most. In this study, we showed that D. minowai adults exhibited significantly higher attraction to mixtures of p-anisaldehyde, eugenol, and farnesene compared to an equivalent dose of p-anisaldehyde alone in H-tube olfactometer assays under laboratory conditions. Moreover, in field experiments conducted in 2022, rubber septa impregnated with a ternary blend of p-anisaldehyde, eugenol, and farnesene (at 3–4.5 mg and a ratio of 3:1:1) captured the highest number of adults on sticky traps, outperforming traps bailed with individual components or a solvent control over two weeks. Significantly, the mass trapping strategy employing these lures achieved control efficacies ranging from 62.8% to 70.7% when compared to traps without attractant, which achieved control efficacies of only 14.2% to 35.4% across three test sites in 2023. These results indicate that the combination of p-anisaldehyde, eugenol, and farnesene exhibits an additive or synergistic effect on D. minowai. In conclusion, our findings establish a theoretical framework and provide practical technological support for integrating attractant-based strategies into comprehensive thrips management strategies.

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An Antennae-Enriched Odorant-Binding Protein EonuOBP43 Mediate the Behavioral Response of the Tea Green Leafhopper, Empoasca onukii Matsuda to the Host and Nonhost Volatiles

Cited by 3 publications

References 50 publications

The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein SvelOBP1 from Sympiezomias velatus (Coleoptera: Curculionidae) to Jujube Volatiles

The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein SvelOBP1 from Sympiezomias velatus (Coleoptera: Curculionidae) to Jujube Volatiles

Construction of a comprehensive evaluation model for constitutive resistance traits of tea cultivars to the pests Matsumurasca onukii and Dendrothrips minowai

Mixture of Synthetic Plant Volatiles Attracts More Stick Tea Thrips Dendrothrips minowai Priesner (Thysanoptera: Thripidae) and the Application as an Attractant in Tea Plantations

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