An annulus fibrosus closure device based on a biodegradable shape-memory polymer network

Sharifi, Shahriar; Kooten, Theo G. van; Kranenburg, Hendrik-Jan C.; Meij, Björn P.; Behl, Marc; Lendlein, Andreas; Grijpma, Dirk W.

doi:10.1016/j.biomaterials.2013.07.061

Cited by 60 publications

(49 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In an extensive review on the use of bioscaffolds and MSCs for annular repair Sherafi et al describe commercially available Inclose surgical meshes, Xclose suturing closures and Barricaid woven mesh barrier devices made from polyethylene terephthalate for the closure and containment of annular defects. Annular closure devices based on a temperature sensitive biodegradable shape‐memory polymer network and photo‐crosslinkable poly(trimethylene carbonate)‐based macromers for closure of ruptured IVDs have also been developed . Despite promising results with these annular containment products recurrent disc herniations still frequently occurred and were common causes of back pain.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of administered mesenchymal stem cells in the initiation and co‐ordination of repair processes by resident disc cells in an ovine (Ovis aries) large destabilizing lesion model of experimental disc degeneration

et al. 2018

View full text Add to dashboard Cite

Background Forty percent of low back pain cases are due to intervertebral disc degeneration (IVDD), with mesenchymal stem cells (MSCs) a reported treatment. We utilized an ovine IVDD model and intradiscal heterologous MSCs to determine therapeutic efficacy at different stages of IVDD. Methodology Three nonoperated control (NOC) sheep were used for MSC isolation. In 36 sheep, 6 × 20 mm annular lesions were made at three spinal levels using customized blades/scalpel handles, and IVDD was allowed to develop for 4 weeks in the Early (EA) and late Acute (LA) groups, or 12 weeks in the chronic (EST) group. Lesion IVDs received injections of 10 × 10 6 MSCs or PBS, and after 8 (EA), 22 (LA) or 14 (EST) weeks recuperation the sheep were sacrificed. Longitudinal lateral radiographs were used to determine disc heights. IVD glycosaminoglycan (GAG) and hydroxyproline contents were quantified using established methods. An Instron materials testing machine and customized jigs analyzed IVD (range of motion, neutral zone [NZ] and stiffness) in flexion/extension, lateral bending and axial rotation. qRTPCR gene profiles of key anabolic and catabolic matrix molecules were undertaken. Toluidine blue and hematoxylin and eosin stained IVD sections were histopathologically scoring by two blinded observers. Results IVDD significantly reduced disc heights. MSC treatment restored 95% to 100% of disc height, maximally improved NZ and stiffness in flexion/extension and lateral bending in the EST group, restoring GAG levels. With IVDD qRTPCR demonstrated elevated catabolic gene expression ( MMP2/3/9/13, ADAMTS4/5 ) in the PBS IVDs and expession normalization in MSC‐treated IVDs. Histopathology degeneracy scores were close to levels of NOC IVDs in MSC IVDs but IVDD developed in PBS injected IVDs. Discussion Administered MSCs produced recovery in degenerate IVDs, restored disc height, composition, biomechanical properties, down regulated MMPs and fibrosis, strongly supporting the efficacy of MSCs for disc repair.

show abstract

Section: Discussionmentioning

confidence: 99%

Efficacy of administered mesenchymal stem cells in the initiation and co‐ordination of repair processes by resident disc cells in an ovine (Ovis aries) large destabilizing lesion model of experimental disc degeneration

et al. 2018

View full text Add to dashboard Cite

show abstract

“…(11) For this reason, extensive research involving injectablebiomaterial candidates for NP regeneration have been explored, but surprisingly a limited number of similar systems have been targeted for annulus fibrosus tissue to date. (12)(13)(14)(15)(16) High-density injectable collagen gels have been investigated to fill punctured AF tissue in a rat tail model. Although no further sign of disc degeneration was observed in terms of hydration, as evaluated using MRI and disc height measurements post-implantation, incomplete AF tissue regeneration was observed in the inner region of the newly formed tissue, combined with a fibrous capsule in the outer part with dissimilarity in terms of extracellular matrix (ECM) composition compared to native AF tissue.…”

Section: Introductionmentioning

confidence: 99%

Enhancing cell migration in shape-memory alginate–collagen composite scaffolds: In vitro and ex vivo assessment for intervertebral disc repair

et al. 2014

View full text Add to dashboard Cite

Lower lumbar disc disorders pose a significant problem in an ageing society with substantial socioeconomic consequences. Both inner tissue (nucleus pulposus, NP) and outer tissue (annulus fibrosus, AF) of the intervertebral disc (IVD) are affected by such debilitating disorders and can lead to disc herniation and lower back pain. In this study, we developed an alginate-collagen composite porous scaffold with shape-memory properties to fill defects occurring in AF tissue of degenerated IVDs, which has the potential to be administered using minimal invasive surgery. In the first part of this work we assessed how collagen incorporation on pre-formed alginate scaffolds influences the physical properties of the final composite scaffold. We also evaluated the ability of AF cells to attach, migrate and proliferate on the composite alginate-collagen scaffolds compared to control scaffolds (alginate only). In vitro experiments, performed in IVD-like microenvironmental conditions (low glucose and low oxygen concentrations), revealed that for alginate only scaffolds, AF cells agglomerated in clusters with limited infiltration and migration capacity. In comparison, for alginatecollagen scaffolds, AF cells readily attached and colonized constructs, while preserving their typical fibroblastic-like cell morphology with spreading behavior and intense cytoskeleton expression. In a second part of this study, we investigated the effects of alginate-collagen scaffold when seeded with bone marrow derived mesenchymal stem cells (MSCs). In vitro, we observed that alginate-collagen porous scaffolds supported cell proliferation and extracellular matrix (ECM) deposition (collagen type I); which secretion was amplified by the local release of TGF-ß3. In addition, when cultured in ex vivo organ defect model, alginatecollagen scaffolds maintained viability of transplanted MSCs for up to 5 weeks. Taken together, these findings illustrate the advantages of incorporating collagen as a means to enhance cell migration and proliferation in porous scaffolds which could be used to augment tissue repair strategies.

show abstract

“…Shape memory polyurethane (SMPU) is a typical shape memory polymer widely used in biomaterials, such as bone tissue engineering,orthodontic, closure device, and self‐expandable stents . The shape memory behavior, typically remembering a temporary shape and returning to its original shape, can be stimulated by pH, temperature, humidity, electricity, magnetism, electromagnetism, and so on .…”

Section: Introductionmentioning

confidence: 99%

Shape Memory Properties and Enzymatic Degradability of Poly(ε‐caprolactone)‐Based Polyurethane Urea Containing Phenylalanine‐Derived Chain Extender

Wang

Zhang

Lin

et al. 2018

Macromolecular Bioscience

View full text Add to dashboard Cite

Biodegradable shape memory polymers are promising biomaterials for minimally invasive surgical procedures. Herein, a series of linear biodegradable shape memory poly(ε-caprolactone) (PCL)-based polyurethane ureas (PUUs) containing a novel phenylalanine-derived chain extender is synthesized. The phenylalanine-derived chain extender, phenylalanine-hexamethylenediamine-phenylalanine (PHP), contains two chymotrypsin cleaving sites to enhance the enzymatic degradation of PUUs. The degradation rate, the crystallinity, and mechanical properties of PUUs are tailored by the content of PHP. Meanwhile, semicrystalline PCL is not only hydrolytically degradable but also vital for shape memory. Good shape memory ability under body temperature is achieved for PUUs due to the strong interactions in hard segments for permanent crosslinking and the crystallization-melt transition of PCL to switch temporary shape. The PUUs would have a great potential in application as implanting stent.

show abstract

An annulus fibrosus closure device based on a biodegradable shape-memory polymer network

Cited by 60 publications

References 31 publications

Efficacy of administered mesenchymal stem cells in the initiation and co‐ordination of repair processes by resident disc cells in an ovine (Ovis aries) large destabilizing lesion model of experimental disc degeneration

Efficacy of administered mesenchymal stem cells in the initiation and co‐ordination of repair processes by resident disc cells in an ovine (Ovis aries) large destabilizing lesion model of experimental disc degeneration

Enhancing cell migration in shape-memory alginate–collagen composite scaffolds: In vitro and ex vivo assessment for intervertebral disc repair

Shape Memory Properties and Enzymatic Degradability of Poly(ε‐caprolactone)‐Based Polyurethane Urea Containing Phenylalanine‐Derived Chain Extender

Contact Info

Product

Resources

About