An animal model for cell-mediated immune responses to dermatophytes

Hunjan, B.S.; Cronholm, Lois S.

doi:10.1016/0091-6749(79)90132-5

Cited by 14 publications

(8 citation statements)

References 10 publications

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“…In this model, it is known that, as in cats, the resolution of the disease and the resistance to reinfection is correlated with the development of delayed-type hypersensitivity (DTH) [15,16]. However, most studies were dealing with Trichophyton spp.…”

Section: Introductionmentioning

confidence: 99%

Humoral and cellular immune response to a crude exo-antigen and purified keratinase ofMicrosporum canisin experimentally infected guinea pigs

Mignon¹,

Leclipteux²,

Focant³

et al. 1999

Med Mycol

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In order to understand better the host-parasite relationship and to compare with previous observations in Microsporum canis naturally infected cats, the humoral and cellular immune responses to both a crude exo-antigen and a 31·5 kDa purified keratinase were evaluated in 12 M. canis experimentally infected guinea pigs. Humoral and cellular responses were assessed by ELISA from days 0 to 56 postinfection (PI) and by measurement of delayed-type hypersensitivity (DTH) responses on days 14 and 57 PI, respectively. Additionally, immunohistochemical staining was performed and demonstrated that the keratinase was produced in infected guinea pig skin, as previously reported in cats. Despite a marked interindividual variation, all the guinea pigs produced specific IgG to the crude exo-antigen from day 21 PI onwards, but no anti-keratinase IgG was detected. Strongly positive DTH responses to the exo-antigen were observed on both dates, whereas the keratinase elicited no and weak DTH on days 14 and 57 PI, respectively. These results are in agreement with those previously described for naturally infected cats, and indicate that the 31·5 kDa keratinase is not a major antigen in M. canis infection.

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Section: Introductionmentioning

confidence: 99%

Humoral and cellular immune response to a crude exo-antigen and purified keratinase ofMicrosporum canisin experimentally infected guinea pigs

Mignon¹,

Leclipteux²,

Focant³

et al. 1999

Med Mycol

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“…In dermatophyte infections the type and duration of specific hypersensitivity is influenced by the fungal species [12,25]. The lack of a standardized method for preparing and testing trichophytin is a serious drawback for studies on the hypersensitivity phenomenon in dermatophytosis.…”

Section: Discussionmentioning

confidence: 99%

“…Specific hypersensitivity is a manifestation of cell-mediated immunity which plays a key role in defence against fungal skin infections [7,10,12,16,20], and is, therefore, a relevant part of studies on the immunological response of animals with dermatophytosis, under both natural and experimental conditions, or of animals immunized with specific vaccines. This work was undertaken because of the apparent absence of studies on the persistence of specific hypersensitivity during infection with Trichophyton equinum.…”

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confidence: 99%

“…In skin tests with three trichophytins it was shown that specific hypersensitivity resulting from infection of laboratory animals persists for at least 6 months. Stronger local reactions were produced with homologous trichophytins, especially in the final tests after 6 months.In humans and in animals, dermatophyte infections induce a specific hypersensitivity which depends on the clinical form, virulence and sensitizing properties of the dermatophyte and also on the type, species and the individual responsiveness of the host [7,12,18,25]. Specific hypersensitivity is a manifestation of cell-mediated immunity which plays a key role in defence against fungal skin infections [7,10,12,16,20], and is, therefore, a relevant part of studies on the immunological response of animals with dermatophytosis, under both natural and experimental conditions, or of animals immunized with specific vaccines.…”

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Hypersensitivity to trichophytin in small animals experimentally infected withTrichophyton equinum

Kostro¹

1989

Med Mycol

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Typical lesions resulting from experimental Trichophyton equinum infection in laboratory animals persisted for 3-4 weeks. Lesions in rabbits were slightly less severe than those in guinea pigs. In experimentally infected dogs lesions persisted for longer and developed in the form of a deep, crusty dermatophytosis. In skin tests with three trichophytins it was shown that specific hypersensitivity resulting from infection of laboratory animals persists for at least 6 months. Stronger local reactions were produced with homologous trichophytins, especially in the final tests after 6 months.In humans and in animals, dermatophyte infections induce a specific hypersensitivity which depends on the clinical form, virulence and sensitizing properties of the dermatophyte and also on the type, species and the individual responsiveness of the host [7,12,18,25]. Specific hypersensitivity is a manifestation of cell-mediated immunity which plays a key role in defence against fungal skin infections [7,10,12,16,20], and is, therefore, a relevant part of studies on the immunological response of animals with dermatophytosis, under both natural and experimental conditions, or of animals immunized with specific vaccines. This work was undertaken because of the apparent absence of studies on the persistence of specific hypersensitivity during infection with Trichophyton equinum. The results may be useful in the development of vaccines, which are not available at present, for the treatment or prevention of ringworm in horses.The purpose of this study was to determine the virulence of T. equinum strains, isolated from horses naturally infected with the dermatophyte in Poland, for laboratory animals and dogs, and also to determine the duration of specific hypersensitivity induced in experimentally infected guinea pigs and rabbits using intradermal tests. METHODS Preparation of the fungal inoculumAnimals were infected with one of two T. equinum strains (339; 414) selected at

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“…Microsporum vanbreuseghem (the zoophilic species) pro¬ duced the most virulent infection and Epidermophyton floccosum (previously considered anthropophilic) produced the mildest infection. 3 Hunjan and Cronholm3 studied immune responses in guinea pigs injected with dermatophytes and reported that infected animals showed delayed hypersensitivity to homologous dermatophyte antigen, which persisted for up to 12 months. 3 Lymphoid cells from Accepted for publication July 15,1981 (Fig 1).…”

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confidence: 99%

Immunology of Human Dermatophyte Infections

Ar¹

1982

Arch Dermatol

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An animal model for cell-mediated immune responses to dermatophytes

Cited by 14 publications

References 10 publications

Humoral and cellular immune response to a crude exo-antigen and purified keratinase ofMicrosporum canisin experimentally infected guinea pigs

Humoral and cellular immune response to a crude exo-antigen and purified keratinase ofMicrosporum canisin experimentally infected guinea pigs

Hypersensitivity to trichophytin in small animals experimentally infected withTrichophyton equinum

Immunology of Human Dermatophyte Infections

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