An Ancient Model Organism to Test In Vivo Novel Functional Nanocrystals

Tortiglione, Claudia

doi:10.5772/21038

Cited by 13 publications

(12 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Groups of 25 polyps were bisected in the upper gastric region and incubated in the presence of different doses of CNO nanoparticles. The polyps were monitored through a stereomicroscope and were grouped in three stages according to their tentacle morphogenetic process: stage zero indicates the complete inhibition of regeneration (zero tentacles); stage 1, indicates heads with aberrant tentacles (one or two), while stage 2 indicates normal regeneration (from four to six tentacles) [ 62 , 66 , 75 ]. No differences were detected between the regeneration efficiency of treated and untreated polyps, as shown in Figure 5 , indicating that the presence of CNOs in Hydra tissues does not impair the regenerative potential.…”

Section: Resultsmentioning

confidence: 99%

“…Over the last years several nanoparticles have been tested for toxicity on Hydra . The results obtained allowed the identification of the pivotal role of not only the chemical composition of the inorganic core, but also of the size, shape and chemical coating of the nanoparticle surfaces [ 61 , 62 , 63 ]. PEG-coated CdSe/CdS quantum rods at the typical concentrations employed for biological imaging applications did not exhibit adverse effects on animal health, making them useful as probes for long-term cell tracking [ 64 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of Carbon Nano-Onions on Hydra vulgaris as a Model Organism for Nanoecotoxicology

et al. 2015

Self Cite

View full text Add to dashboard Cite

The toxicological effects of pristine and chemically modified carbon nano-onions (CNOs) on the development of the freshwater polyp Hydra vulgaris were investigated in order to elucidate the ecotoxicological effects of CNOs. Chemical modifications of the CNOs were accomplished by surface functionalization with benzoic acid, pyridine and pyridinium moieties. thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR) and Raman spectroscopy confirmed the covalent surface functionalization of CNOs. Hydra specimens were exposed to the carbon nanomaterials by prolonged incubation within their medium. Uptake was monitored by optical microscopy, and the toxicological effects of the CNOs on Hydra behavior, morphology, as well as the long-term effects on the development and reproductive capability were examined. The obtained data revealed the absence of adverse effects of CNOs (in the range 0.05–0.1 mg/L) in vivo at the whole animal level. Together with previously performed in vitro toxicological analyses, our findings indicate the biosafety of CNOs and the feasibility of employing them as materials for biomedical applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of Carbon Nano-Onions on Hydra vulgaris as a Model Organism for Nanoecotoxicology

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reliable methods have been developed for evaluation of toxicological impact of several nanostructured materials using Hydra, spanning from in vivo analysis (structural morphological changes, impact on reproductive rate, efficiency of regeneration) to in vitro approaches of cell and molecular biology. [11][12][13][14][15][16] Owing to its unique developmental potentials (see Figure S9), the toxicological impact of any medium suspended compound can be estimated using Hydra. The toxicant under investigation can be added to the medium bathing living polyps and the effects on morphology, reproductive and regeneration capabilities can be quantified by standardised protocols.…”

Section: S4 Toxicological Analysis Of Polyelectrolyte Capsules In Hymentioning

confidence: 99%

Control of Wnt/β-Catenin Signaling Pathway in Vivo via Light Responsive Capsules

et al. 2016

Self Cite

View full text Add to dashboard Cite

The possibility to remotely manipulate intracellular pathways in single cells is among the current goals of biomedicine, demanding new strategies to control cell function and reprogramming cell fate upon external triggering. Optogenetics is one approach in this direction, allowing specific cell stimulation by external illumination. Here, we developed optical switchers of an ancient and highly conserved system controlling a variety of developmental and adult processes in all metazoans, from Hydra to mammals, the Wnt/β-catenin signaling pathway. An intracellular modulator of the Wnt pathway was enclosed into polyelectrolyte multilayer microcapsules engineered to include self-tracking (i.e., fluorescence labeling) and light mediated heating functionalities (i.e., plasmonic nanoparticles). Capsules were delivered in vivo to Hydra and NIR triggered drug release caused forced activation of the Wnt pathway. The possibility to remotely manipulate the Wnt pathway by optical switchers may be broadly translated to achieve spatiotemporal control of cell fate for new therapeutic strategies.

show abstract

“…Apart from this brief introduction to the ecotoxicological impact of nanomaterialsa whole scenario on the overall aspects to be considered would be necessary for proper nanoparticle risk assessmentin this chapter we will not go into further details in this field, referring the reader instead to other dedicated reviews [1,8, [28][29][30][31]. Here we will focus on the molecular responses elicited in a cell, considered as the smallest living unit, independently from its origin (human, animal or plant derived) upon nanoparticle exposure.…”

Section: Impact On Environment: Nanoecotoxicologymentioning

confidence: 99%

Molecular Bases of Nanotoxicology

Tino

Ambrosone

Marchesano

et al. 2014

Bio‐ and Bioinspired Nanomaterials

Self Cite

View full text Add to dashboard Cite

The recent design and development of nanosized particulate materials, generally termed as nanomaterials, and their growing employment in several fields spanning from electronics to medicine, created an immediate need for approaches to assess nanomaterial safety. Owing to their distinctive physicochemical and kinetic characteristics, their behavior toward biological systems is influenced by many factors including shape, coatings, solubility, and aggregation of the particles, but is also dictated by the living entity exposed to the nanoparticle, making the prediction of toxicity a great challenge. Owing to the variety of the nanoparticles and systems employed to assess their toxicity, many diverse mechanisms have been identified that contribute to their adverse effects on cells, tissues, and organs of living organisms, and the relevant literature is exponentially growing. Many studies have reviewed in this fast changing research area, addressing several aspects spanning from nanoparticle physicochemical properties that influence toxicity to the impact on environment and human health. Here we focus on the possible molecular events elicited in distinct subcellular compartments by nanomaterials, whose potential hazardous nature is here considered by calling them "nanostressors." We provide an overview of various NP evoked molecular processes generally assembled under the common term nanotoxicology, pointing out the most advanced technological tools (microarray, RNAseq) and underexplored effect (epigenetic) recently employed in the nanotoxicology field. © 2015 Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved

show abstract

An Ancient Model Organism to Test In Vivo Novel Functional Nanocrystals

Cited by 13 publications

References 68 publications

Impact of Carbon Nano-Onions on Hydra vulgaris as a Model Organism for Nanoecotoxicology

Impact of Carbon Nano-Onions on Hydra vulgaris as a Model Organism for Nanoecotoxicology

Control of Wnt/β-Catenin Signaling Pathway in Vivo via Light Responsive Capsules

Molecular Bases of Nanotoxicology

Contact Info

Product

Resources

About