The feasibility of using ultrafiltration (UF) to eliminate phytate from aqueous sodium chloride extracts of rapeseed was studied. With a proper choice of casting solution composition and casting conditions, high flux asymmetric cellulose acetate membranes of different surface porosity capable of giving different levels of phytate‐protein separation were effectively created. The efficiency of the cast cellulose acetate membranes on phytate‐protein separation was greatly influenced by the solution pH. The presence of sodium chloride in the processed solution was important to obtain a low retention of phytate. Complete removal of phytate from rapeseed extract with little or no loss of proteins was achieved using continuous diafiltrarion.