An Analysis of Transvection at the yellow Locus of Drosophila melanogaster

Morris, James R.; Chen, Ji‐Long; Filandrinos, Stephen; Dunn, R. C.; Fisk, Ridgely; Geyer, Pamela; Wu, Chao-ting

doi:10.1093/genetics/151.2.633

Cited by 49 publications

(1 citation statement)

References 94 publications

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“…The pairing of homologs in somatic tissues enables regulatory elements on one homolog to exert their effects on the opposite homolog, resulting in genetic complementation in trans and the normal expression of a gene. Several genes have been observed to be subject to trans effects in Drosophila, including Ultrabithorax (Ubx) [125], yellow (y) [126,127], decapentaplegic (dpp) [128], cubitus interruptus (ci) [129], brown (bw) [130], engrailed (en) [131], eyes absent (eya) [132], Gpdh [133], hedgehog (hh) [134], pointed (pnt) [135], sex combs reduced (scr) [136], TMR of Abd-B [137], spineless (ss) [138], vestigial (vg) [139], white (w) [140], and wingless (wg) [141].…”

Section: Transvection and Interhomolog Communicationmentioning

confidence: 99%

Multi-Scale Organization of the Drosophila melanogaster Genome

Peterson

Samuelson

Hanlon

2021

Genes

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Interphase chromatin, despite its appearance, is a highly organized framework of loops and bends. Chromosomes are folded into topologically associating domains, or TADs, and each chromosome and its homolog occupy a distinct territory within the nucleus. In Drosophila, genome organization is exceptional because homologous chromosome pairing is in both germline and somatic tissues, which promote interhomolog interactions such as transvection that can affect gene expression in trans. In this review, we focus on what is known about genome organization in Drosophila and discuss it from TADs to territory. We start by examining intrachromosomal organization at the sub-chromosome level into TADs, followed by a comprehensive analysis of the known proteins that play a key role in TAD formation and boundary establishment. We then zoom out to examine interhomolog interactions such as pairing and transvection that are abundant in Drosophila but rare in other model systems. Finally, we discuss chromosome territories that form within the nucleus, resulting in a complete picture of the multi-scale organization of the Drosophila genome.

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Section: Transvection and Interhomolog Communicationmentioning

confidence: 99%

Multi-Scale Organization of the Drosophila melanogaster Genome

Peterson

Samuelson

Hanlon

2021

Genes

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Disruption of the genomic imprint in trans with homologous recombination at Snrpn in ES cells

Tsai

Bressler

Jiang

et al. 2003

Genesis

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In gene targeting studies of the Prader-Willi syndrome (PWS)/Angelman syndrome (AS) domain in mouse ES cells, we recovered only recombinants with the paternal allele for constructs at exons 2 or 3 of the imprinted, maternally silenced Snurf-Snrpn gene. These sites lie close to the imprinting center (IC) for this domain. In contrast, recombinants for Ube3a within the same imprinted domain were recovered with equal frequency on the maternal and paternal alleles. In addition, gene targeting of the paternal allele for Snurf-Snrpn resulted in partial or complete demethylation in trans with activation of expression for the previously silenced maternal allele. The imprint switching of the maternal allele in trans is not readily explained by competition for trans-acting factors and adds to a growing body of evidence indicating homologous association of oppositely imprinted chromatin domains in somatic mammalian cells.

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New reproductive anomalies in fruitless‐mutant Drosophila males: Extreme lengthening of mating durations and infertility correlated with defective serotonergic innervation of reproductive organs

et al. 2001

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Several features of male reproductive behavior are under the neural control of fruitless (fru) in Drosophila melanogaster. This gene is known to influence courtship steps prior to mating, due to the absence of attempted copulation in the behavioral repertoire of most types of fru-mutant males. However, certain combinations of fru mutations allow for fertility. By analyzing such matings and their consequences, we uncovered two striking defects: mating times up to four times the normal average duration of copulation; and frequent infertility, regardless of the time of mating by a given transheterozygous fru-mutant male. The lengthened copulation times may be connected with fru-induced defects in the formation of a male-specific abdominal muscle. Production of sperm and certain seminal fluid proteins are normal in these fru mutants. However, analysis of postmating qualities of females that copulated with transheterozygous mutants strongly implied defects in the ability of these males to transfer sperm and seminal fluids. Such abnormalities may be associated with certain serotonergic neurons in the abdominal ganglion in which production of 5HT is regulated by fru. These cells send processes to contractile muscles of the male's internal sex organs; such projection patterns are aberrant in the semifertile fru mutants. Therefore, the reproductive functions regulated by fruitless are expanded in their scope, encompassing not only the earliest stages of courtship behavior along with almost all subsequent steps in the behavioral sequence, but also more than one component of the culminating events.

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An Analysis of Transvection at the yellow Locus of Drosophila melanogaster

Cited by 49 publications

References 94 publications

Multi-Scale Organization of the Drosophila melanogaster Genome

Multi-Scale Organization of the Drosophila melanogaster Genome

Disruption of the genomic imprint in trans with homologous recombination at Snrpn in ES cells

New reproductive anomalies in fruitless‐mutant Drosophila males: Extreme lengthening of mating durations and infertility correlated with defective serotonergic innervation of reproductive organs

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