An Analysis of the Wood Sugar Assay Using HPLC: A Comparison with Paper Chromatography

Pettersen, Roger C.; Schwandt, Virgil H.; Effland, Marilyn J.

doi:10.1093/chromsci/22.11.478

Cited by 80 publications

(24 citation statements)

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“…Klason lignin and individual wood sugars were determined following the procedures of Effland (1977) and Pettersen et al (1985). The type of lignin occurring in the decayed wood was estimated by chemical oxidation with nitrobenzene (Obst 1982) and cupric oxide (Hedges and Ertel 1982).…”

Section: Methodsmentioning

confidence: 99%

Resistance of hardwood vessels to degradation by white rot Basidiomycetes

Blanchette¹,

Obst²,

Hedges³

et al. 1988

Can. J. Bot.

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BLANCHETTE, R. A,, OBST, J. R., HEDGES, J. I., and WELIKY, K. 1988. Resistance of hardwood vessels to degradation by white rot Basidiomycetes. Can. J. Bot. 66: 1841 -1847. White stringy rot, an unusual type of selective fungal decay, can be found in wood of some dicotyledonous angiosperms. Stages of advanced decay consist of a mass of vessel elements with only remnants of other cells adhering to the vessel walls. Degradation by various white rot Basidiomycetes causes loss of fibers, fiber tracheids, and parenchyma cells but not vessels. In wood of Acacia koa var. koa with a white pocket rot caused by Phellinus kawakamii, fibers and parenchyma cells were preferentially delignified. After extensive lignin removal the cellulose remaining in the secondary wall was degraded. Large vessel elements remained relatively intact after other cells were completely degraded. The resistance of vessels to degradation appears to be due to their high 1ignin:carbohydrate ratio, lignin monomer composition, and cell wall morphology. BLANCHETTE, R. A., OBST, J. R., HEDGES, J. I., et WELIKY, K. 1988. Resistance of hardwood vessels to degradation by white rot Basidiomycetes. Can. J. Bot. 66 : 1841 -1847. Dans le bois de certaines angiospermes dicotylCdones, on trouve une pourriture blanche B cordons, un type peu frCquent de carie fongique selective. Aux stades avancCs, la carie consiste en une masse d'C1Cments de vaisseaux accompagnCe seulement de rCsidus d'autres cellules qui adhbrent aux parois de ces vaiseaux. La biodegradation par divers basidiomycbtes de carie blanche conduit B la destruction des fibres, des fibro-trachEides et des cellules de parenchyme mais non B celle des vaisseaux. Dans le bois d'Acacia koa var. koa les auteurs ont trouvC une poche de carie blanche causCe par le Phellinus kawakamii, oh les fibres et les cellules de parenchymes sont ~Clectivement dClignifiCe. Ce n'est qu'aprbs une Climination selective de la lignine que la cellulose rCsiduelle de la paroi cellulaire secondaire est dCgradCe. Les grands ClCments de vaisseaux demeurent relativement intacts alors que les autres types de cellules sont complktement dCgradCs. La resistance des vaisseaux B la degradation semble liCe B leur rapport 1ignine:glucide ClevC, B la composition des monombres de lignine et B la morphologie des parois cellulaires.[Traduit par la revue]

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Section: Methodsmentioning

confidence: 99%

Resistance of hardwood vessels to degradation by white rot Basidiomycetes

Blanchette¹,

Obst²,

Hedges³

et al. 1988

Can. J. Bot.

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“…Neutral sugars were separated by high-performance ion-exchange chromatography (Petersen et al 1984) using a lead form column (Supelcogel Pb, Supelco Inc., Bellefonte, PA). Neutral sugars were separated by high-performance ion-exchange chromatography (Petersen et al 1984) using a lead form column (Supelcogel Pb, Supelco Inc., Bellefonte, PA).…”

Section: Compositional Analysesmentioning

confidence: 99%

Molecular Characterization of Barley β‐Glucans by Size‐Exclusion Chromatography with Multiple‐Angle Laser Light Scattering and Other Detectors

1997

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Cereal Chem. 74(5):599-604Molecular characteristics were determined for mixed-linkage (1→3) (1→4)-β-D-glucans (β-glucans) extracted from Azhul, Crystal, Waxbar, and Prowashonupana barleys. β-Glucans in extracts (with or without αamylase, protease, hemicellulase, or xylanase treatment) were separated from other components by high-performance size-exclusion chromatography and detected with multiple-angle laser light scattering, refractive index, and fluorometry following postrefractive index treatment with Calcofluor. Pretreatment of barley with 70% ethanol (80°C, 4 hr) reduced β-glucanase activity by ~20%. Hot-alcohol treatment also reduced β-glucan extraction at 23 and 65°C by 42 and 14%, respectively. Molecular weights of β-glucans in the first water extract were generally higher than in succeeding water and alkali extracts. Weight average molecular weights ranged from 0.44 × 10 6 to 2.34 × 10 6 g/mol after α-amylase treatment to remove interfering starch. Interference due to pentosans was not demonstrated using enzyme treatments.

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“…Glucose was determined by high pressure liquid chromatography after complete acid hydrolysis and neutralization with Ba(OH) 2 (Pettersen et al 1984).…”

Section: Glucose Yield After Acid Hydrolysismentioning

confidence: 99%