An Analysis of IL-10, IL-17A, IL-17RA, IL-23A and IL-23R Expression and Their Correlation with Clinical Course in Patients with Psoriasis

Abstract

Being one of the most common dermatological inflammatory disorders, psoriasis is a frequent subject of research. It is considered to be a T cell-dependent immune disease whose pathogenesis is influenced by cytokines, such as IL-10, IL-17A, IL-17RA, IL-23A and IL-23R. The present study examines whether the expression of selected genes is correlated with the clinical course of psoriasis, assessed by the PASI, BSA and DLQI scales. Skin biopsies and blood from 60 patients with psoriasis and 24 healthy controls were obtained for RNA isolation. These were subjected to RT-PCR for IL-10, IL-17A, IL-17RA, IL-23A and IL-23R genes. The results were presented as an RQ value. IL-17A and IL-23R expression levels were higher in psoriatic skin compared to controls, while IL-10 expression was lower. A positive correlation was also found between RQ for IL-23A and PASI index. Psoriatic skin is characterised by elevated expression of IL-17A and IL-23R and decreased expression of IL-10. This indicates that the selected cytokines may be one of the factors involved in the pathogenesis and pathomechanism of psoriasis, but more studies need to be made before we can elucidate the exact reason for the unbalance in cytokine expression levels.

Levels of IL-23/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New Classification

“…In contrast, other authors have described an increment in the concentration of IL-17A in different biological fluid samples from periodontitis patients [17,18,26], while others still failed to detect this cytokine in patients with periodontitis [12,13]. However, IL-17A is a cytokine highly studied in inflammatory and autoimmune diseases such as Sjögren's syndrome and rheumatoid arthritis, among others [43][44][45][46]. In this sense, Ridgley LA.…”

IL‐23/IL‐17 immune axis mediates the imiquimod‐induced psoriatic inflammation by activating ACT1/TRAF6/TAK1/NF‐κB pathway in macrophages and keratinocytes

“…This immunomodulatory cytokine is a central anti‐inflammatory mediator that reduces tissue damage caused by excessive and uncontrolled inflammatory stimulation and protects the host from an excessive inflammatory response 15 . Studies found that IL‐10 level was decreased in patients with psoriasis compared to healthy controls 16 . Interestingly, patients during established antipsoriatic therapy showed a higher IL‐10 mRNA expression of peripheral blood mononuclear cells than patients before the therapy 17 .…”

“…15 Studies found that IL-10 level was decreased in patients with psoriasis compared to healthy controls. 16 Interestingly, patients during established antipsoriatic therapy showed a higher IL-10 mRNA expression of peripheral blood mononuclear cells than patients before the therapy. 17 In the present study, we observed decreased expression of IL-10 in the skin of IMQ-treated mice compared to healthy mice.…”