An analogue of piperonyl butoxide facilitates the characterisation of metabolic resistance

Moores, G. D.; Philippou, Despina; Borzatta, Valerio; Trincia, Paolo; Jewess, P. J.; Gunning, Robin V.; Bingham, Georgina

doi:10.1002/ps.1661

Cited by 50 publications

(46 citation statements)

References 17 publications

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“…The higher susceptibility to chlorpyrifos-ethyl in the pyrethroid resistant populations probably relates to the prevailing mechanism of resistance detected in populations from Central Europe (Philippou et al 2011). That is metabolic resistance which is based mainly on enhanced oxi- Oxidative enzymes (cytochrome P 450 monooxygenases) play the most important role in detoxification of pyrethroids in insect bodies (Moores et al 2009;Philippou et al 2011). High synergy of pyrethroid insecticides with piperonyl butoxid (the inhibitor of oxidative enzymes) and low synergy with carbaryl and tributyltin acetate (Wegorek et al 2007) confirm the above statements.…”

Section: Discussionmentioning

confidence: 99%

Negative correlations between the susceptibilities of Czech and Slovak pollen beetle populations to lambda-cyhalothrin and chlorpyrifos-ethyl in 2014 and 2015

Seidenglanz¹,

Poslušná²,

Kolařík³

et al. 2017

Plant Prot. Sci.

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Section: Discussionmentioning

confidence: 99%

Negative correlations between the susceptibilities of Czech and Slovak pollen beetle populations to lambda-cyhalothrin and chlorpyrifos-ethyl in 2014 and 2015

Seidenglanz¹,

Poslušná²,

Kolařík³

et al. 2017

Plant Prot. Sci.

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“…PBO is also known to block nonspecific esterases in some insect species. [20][21][22] The involvement of nonspecific esterases in the cypermethrin resistance of T. tabaci must also be examined. 14) reported that most strains encoding T929I are arrhenotokous.…”

Section: Discussionmentioning

confidence: 99%

Cypermethrin resistance and reproductive types in onion thrips, <i>Thrips tabaci</i> (Thysanoptera: Thripidae)

Aizawa

Watanabe²,

Kumano³

et al. 2016

J. Pestic. Sci.

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Cypermethrin resistance and reproductive types were examined for T. tabaci strains. Some arrhenotokous and thelytokous strains encoded the sodium channel mutation (T929I) involved in cypermethrin resistance. However, the resistance levels varied to some degree among the strains. A cytochrome P450 inhibitor, piperonyl butoxide, showed different synergistic effects on the strains examined. These results suggest that fundamental and additional levels of cypermethrin resistance in T. tabaci are conferred respectively by reduced sensitivity of the sodium channel and by cytochrome P450-mediated detoxification.

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“…In contrast, esterase inhibition by OPs occurs 0.25-4 hr after in vivo application. 42) Gunning et al 69) and Moores et al 63) identified two ana-…”

Section: Heliothine Resistance To Spsmentioning

confidence: 99%

“…This is because H. armigera esterases are also inhibited by PBO. [59][60][61][62][63][64] However another study on a different species only saw this effect at high concentrations of PBO (3 mM) of dubious physiological relevance, whereas DEF at concentrations 100-fold lower shows a similar effect. 65) Other evidence that PBO does not have high affinity for the esterases is that the addition of PBO to extracts containing the H. armigera esterases prior to separation by native PAGE does not affect their esterase activity.…”

Section: Heliothine Resistance To Spsmentioning

confidence: 99%

Esterase-based metabolic resistance to insecticides in heliothine and spodopteran pests

Farnsworth

Teese

Yuan

et al. 2010

Journal of Pesticide Science

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IntroductionCarboxylesterases have been implicated in metabolic resistance to several classes of insecticide in a wide variety of pest insect species. [1][2][3] The classes most commonly involved are synthetic pyrethroids (SPs) and organophosphates (OPs), and carbamates (CBs) are also involved in several cases. There are also occasional reports of carboxylesterase phenotypes associated with resistance to benzoylureas, the oxadiazine indoxacarb, the diacylhydrazine tebufenozide, the organochlorine endosulfan and the proteinaceous Cry1Ac toxin of Bacillus thuringiensis that is expressed in transgenic crops. Figure 1 gives structures for some key compounds among these various classes of insecticide.It might be expected that carboxylesterases would be involved in SP resistance because the great majority of SPs are carboxylesters. However, no precise molecular mechanisms for esterase-based SP resistance have yet been elucidated in any insect. OPs are generally phosphotriesters rather than carboxylesters but nevertheless they bind many carboxylesterases with high affinity and this provides a basis for two forms of resistance, both now elucidated at a molecular level in several species. One form involves substantial overexpression of the esterase, allowing for effective sequestration of the insecticide. The other involves specific mutations in the active site of the enzyme which convert it to an OP hydrolase (the so-called "mutant ali-esterase" mechanism). CBs are carbamic acid (NH 2 COOH) derivatives, in which a functional group has been added as an ester; these esters are chemically similar to carboxylesters. Carbamates bind carboxylesterases with quite high affinity, but as yet the molecular mechanisms by which a (mutant) carboxylesterase confers CB resistance have not been elucidated in any species. Of the other insecticide classes occasionally linked to esterase-based resistance only indoxacarb has ester bonds (two Elevated esterase activities and increased band intensities of multiple esterase isozymes after electrophoresis are commonly associated with resistance to organophosphate, pyrethroid and carbamate insecticides in various heliothine and spodopteran pests. One possible explanation for this involves a 'master regulator' mutation in a more general chemical stress response. An association between elevated esterase activities and isozyme intensities has also been reported for resistance to the Cry1Ac toxin of Helicoverpa armigera. The basis for this is unclear albeit some involvement of esterases could be mediated by the toxin's affinity for N-acetyl galactosamine glycans on certain gut-expressed esterases in this species.

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An analogue of piperonyl butoxide facilitates the characterisation of metabolic resistance

Cited by 50 publications

References 17 publications

Negative correlations between the susceptibilities of Czech and Slovak pollen beetle populations to lambda-cyhalothrin and chlorpyrifos-ethyl in 2014 and 2015

Negative correlations between the susceptibilities of Czech and Slovak pollen beetle populations to lambda-cyhalothrin and chlorpyrifos-ethyl in 2014 and 2015

Cypermethrin resistance and reproductive types in onion thrips, <i>Thrips tabaci</i> (Thysanoptera: Thripidae)

Esterase-based metabolic resistance to insecticides in heliothine and spodopteran pests

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