An amperometric sensor employing glucose oxidase immobilized on nylon membranes with different pore diameter and grafted with different monomers

Portaccio, Marianna; El-Masry, M.M.; Diano, Nadia; Maio, Anna De; Grano, V.; Lepore, Maria; Travascio, Paola; Bencivenga, U.; Pagliuca, N.; Mita, D.G.

doi:10.1016/s1381-1177(02)00058-9

Cited by 28 publications

(15 citation statements)

References 29 publications

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“…In particular, nylon supports have been extensively used for biosensing applications due to their good mechanical properties and biocompatibility. [2] However, commercially available nylon films exhibit insulating properties and they are not suitable for biosensing application without a preliminary chemical or physical treatment. [3] Furthermore, nylon films are generally weak to bind enzymes without specific chemical treatments, like hydrolyzation [4], grafting [2] or alkylation.…”

Section: Introductionmentioning

confidence: 99%

“…[2] However, commercially available nylon films exhibit insulating properties and they are not suitable for biosensing application without a preliminary chemical or physical treatment. [3] Furthermore, nylon films are generally weak to bind enzymes without specific chemical treatments, like hydrolyzation [4], grafting [2] or alkylation. [5] Recent advances on nylon membranes for biosensing applications consist in their production by electrospinning in the form of nanofibrous assemblies.…”

Section: Introductionmentioning

confidence: 99%

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Nylon Nanofibrous Biosensors for Glucose Determination

et al. 2010

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Nanofibrous membranes have been produced by electrospinning to develop first generation glucose biosensors. The direct immobilization of glucose oxidase onto the polyamide nanofibrous surfaces by drop coating revealed a simple and efficient method for the development of sensitive, stable, and reproducible electrochemical biosensors. The biosensor showed a linear response over the range 1 – 9X10-3 glucose (R-2=0.9997) with a sensitivity of 1.11 muA/mM and a limit of detection of 2.5X10-6 M (S/N=3). The uncertainty of repeatability was 2% (RSD%, n=30). After one month of storage, the signal decreased of 35%. The recovery of glucose, evaluated in real samples of honey, was 98% (RSD%=1%, n=3)

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nylon Nanofibrous Biosensors for Glucose Determination

et al. 2010

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“…After this step, the pH solution dependence of enzyme reaction was investigated. Since an enzyme reaction is strongly dependent on the pH of the solution, especially when the catalyst is immobilized [30][31][32][33][34], the biosensor response was studied at different values of the pH in the range from 3.5 to 6.5. In Figures 4(a) and 4(b), the results of this investigation are reported as the relative values of peak as a function of pH for lactose and glucose, respectively.…”

Section: Resultsmentioning

confidence: 99%

Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor

Portaccio

Lepore

2017

Journal of Sensors

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A three-electrode amperometric biosensor for the detection of two different saccharides (lactose and glucose) in aqueous solutions is described. On the graphite working electrode, the glucose oxidase (GOD) and -galactosidase ( -gal) were coimmobilized by means of covalent bonding. The response of the biosensor as a function of the relative concentration of the two immobilized enzymes was investigated and the best working conditions were identified by measuring the sensitivity and the linear range response. In particular, our best lactose biosensor shows a linear range up to 0.010 mM and a limit of detection (LOD) and a sensitivity equal to 0.001 mM and 850 ± 81 A/mM, respectively. For glucose, the values of the above-mentioned parameters are equal to 0.015 mM for the linear range, 0.001 mM for LOD, and 505 ± 55 A/mM for the sensitivity. The working parameters of our biosensors are significant in comparison with other biosensors developed for concentration determination of the two saccharides investigated in the present work. In particular, low (LOD) and high sensitivities are obtained for lactose and glucose. To challenge our biosensor with real samples, it was tested using fruit juices, skim milk, and whey.

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“…These latter results are similar to those obtained by other authors that, in order to increase the linear range of their calibration curves, decreased the substrate diffusive flux towards the bioactive element of the biosensor. Decreased diffusion was obtained by employing multi-layers of the same membrane [21,22] or membranes of similar thickness with different pore diameters [23].…”

Section: Measurements In Aqueous Solutionsmentioning

confidence: 99%

Amperometric Glucose Determination by Means of Glucose Oxidase Immobilized on a Cellulose Acetate Film: Dependence on the Immobilization Procedures

et al. 2007

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Glucose microelectrodes were prepared by immobilizing glucose oxidase onto a cellulose acetate film coating a platinum wire. Hexamethylenediamine (HMDA) and Glutaraldehyde (GA) were employed as spacer and coupling agent, respectively. Sensitivities and linear response ranges were studied as a function of the relative amounts of HMDA and GA. The best sensitivity was found when HMDA and GA were 5% and 2.5% in aqueous solutions, respectively. Taking as a reference the functioning of this biosensor, the roles of HMDA and GA percentages appear to be opposed when the extension of the linear response range is considered. Indeed, an increase of one unit in HMDA percentage (from 5 to 6 %) induces an increase in the extension of the linear response range equal to that obtained with a decrease of one unit of GA percentage (from 2.5 to 1.5%). The functioning of the biosensor displaying the highest sensitivity was tested with pineapple juice and rat serum, giving results comparable to those obtained by means of the glu-cinet test. Moreover, the in vivo glucose measurement during induced glycaemia in Sprague Dawley rats by intraperitoneal glucose injections resulted similar to that obtained by using a commercial glucometer.

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An amperometric sensor employing glucose oxidase immobilized on nylon membranes with different pore diameter and grafted with different monomers

Cited by 28 publications

References 29 publications

Nylon Nanofibrous Biosensors for Glucose Determination

Nylon Nanofibrous Biosensors for Glucose Determination

Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor

Amperometric Glucose Determination by Means of Glucose Oxidase Immobilized on a Cellulose Acetate Film: Dependence on the Immobilization Procedures

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