An amperometric glucose biosensor based on electrostatic force induced layer-by-layer GOD/chitosan/pyrite on a glassy carbon electrode

Ma, T.; Wang, Yue; Ying, Hanjie; Wang, Enlei; Yin, Guangzhi; Hasebe, Yasushi; Zhang, Zhiqiang

doi:10.2116/analsci.21p250

Cited by 7 publications

(5 citation statements)

References 61 publications

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“…Among all, polyamidoamine dendrimers have generally found application in biosensors construction and cancer cell targeting due to their large number of amine groups available for biomolecule immobilization. In their study, authors have used the advantages of polyamidoamine dendrimers to increase the surface area, thus allowing the immobilization of higher amounts of glucose oxidase and the achievement of direct electron transfer between FAD on the enzyme and the electrodes [105] (Figure 5). Another glucose biosensor, GOD/CS/PR/GCE, was suggested by Ma et al [106] in their study, an amperometric glucose biosensor based on glucose oxidase immobilization with a layer-by-layer method and chitosan and pyrite, tightly adsorbed through electrostatic force on a glassy carbon electrode.…”

Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

“…Moreover, PR concentration for detecting 20 mM glucose, GOD concentrations, pH, chitosan concentration, and mediator type were optimized. The linear Another glucose biosensor, GOD/CS/PR/GCE, was suggested by Ma et al [105] in their study, an amperometric glucose biosensor based on glucose oxidase immobilization with a layer-by-layer method and chitosan and pyrite, tightly adsorbed through electrostatic force on a glassy carbon electrode. The immobilization method provided good bioelectrocatalytic activity of GOD on the CS-and PR-modified electrode surface.…”

Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

“…The linear range of detection obtained using the designed biosensor was 0.5–60 mM, and the detection limit was 50 μM glucose. As a result, this process of using pyrite and chitosan as physically modified GOD could be helpful in designing better enzyme-based biosensors for a wide variety of practical applications [ 105 ].…”

Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

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Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

Kilic,

Singh,

Keles

et al. 2023

Biosensors

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Electrochemistry is a genuinely interdisciplinary science that may be used in various physical, chemical, and biological domains. Moreover, using biosensors to quantify biological or biochemical processes is critical in medical, biological, and biotechnological applications. Nowadays, there are several electrochemical biosensors for various healthcare applications, such as for the determination of glucose, lactate, catecholamines, nucleic acid, uric acid, and so on. Enzyme-based analytical techniques rely on detecting the co-substrate or, more precisely, the products of a catalyzed reaction. The glucose oxidase enzyme is generally used in enzyme-based biosensors to measure glucose in tears, blood, etc. Moreover, among all nanomaterials, carbon-based nanomaterials have generally been utilized thanks to the unique properties of carbon. The sensitivity can be up to pM levels using enzyme-based nanobiosensor, and these sensors are very selective, as all enzymes are specific for their substrates. Furthermore, enzyme-based biosensors frequently have fast reaction times, allowing for real-time monitoring and analyses. These biosensors, however, have several drawbacks. Changes in temperature, pH, and other environmental factors can influence the stability and activity of the enzymes, affecting the reliability and repeatability of the readings. Additionally, the cost of the enzymes and their immobilization onto appropriate transducer surfaces might be prohibitively expensive, impeding the large-scale commercialization and widespread use of biosensors. This review discusses the design, detection, and immobilization techniques for enzyme-based electrochemical nanobiosensors, and recent applications in enzyme-based electrochemical studies are evaluated and tabulated.

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Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

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Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

Kilic,

Singh,

Keles

et al. 2023

Biosensors

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“…However, the accuracy of the system at that resolution is low. Biosensors based on other types of platforms, for example, screen-printed electrodes [18] and bulk electrodes (CPE [19][20][21] or GCE [22,23]) are also being researched to detect glucose.…”

Section: Introductionmentioning

confidence: 99%

A Noninvasive Sweat Glucose Biosensor Based on Glucose Oxidase/Multiwalled Carbon Nanotubes/Ferrocene-Polyaniline Film/Cu Electrodes

Guan

Liu

et al. 2022

Micromachines

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Diabetes remains a great threat to human beings’ health and its world prevalence is projected to reach 9.9% by 2045. At present, the detection methods used are often invasive, cumbersome and time-consuming, thus increasing the burden on patients. In this paper, we propose a novel noninvasive and low-cost biosensor capable of detecting glucose in human sweat using enzyme-based electrodes for point-of-care uses. Specifically, an electrochemical method is applied for detection and the electrodes are covered with multilayered films including ferrocene-polyaniline (F-P), multi-walled carbon nanotubes (MWCNTs) and glucose oxidase (GOx) on Cu substrates (GOx/MWCNTs/F-P/Cu). The coated layers enhance the immobilization of GOx, increase the conductivity of the anode and improve the electrochemical properties of the electrode. Compared with the Cu electrode and the F-P/Cu electrode, a maximum peak current is obtained when the MWCNTs/F-P/Cu electrode is applied. We also study its current response by cyclic voltammetry (CV) at different concentrations (0–2.0 mM) of glucose solution. The best current response is obtained at 0.25 V using chronoamperometry. The effective working lifetime of an electrode is up to 8 days. Finally, to demonstrate the capability of the electrode, a portable, miniaturized and integrated detection device based on the GOx/MWCNTs/F-P/Cu electrode is developed. The results exhibit a short response time of 5 s and a correlation coefficient R2 of 0.9847 between the response current of sweat with blood glucose concentration. The LOD is of 0.081 mM and the reproducibility achieved in terms of RSD is 3.55%. The sweat glucose sensor is noninvasive and point-of-care, which shows great development potential in the health examination and monitoring field.

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“…For those reasons, accurate glucose quantification with a quick and simple method has driven the search for new testing devices and procedures. Various methods have been reported for GOx immobilization, such as covalent cross-linking, electrochemical polymerization, sol-gel encapsulating, and layerby-layer (LBL) self-assembly based on the electrostatic interaction with polyelectrolytes of opposite charge (Bracamonte et al 2014;Ma et al 2022). This last method has attracted great interest due to its simplicity, wide variety of materials that can be used, and the precise control of the composition and thickness of the layer on the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Multilayers of Water Glucose Modified-Chitosan and Glucose Oxidase for Detection of Glucose in Milk Samples

Gulotta¹,

VERGARA²,

MONTENEGRO³

et al. 2022

SJS

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Background: A crucial aspect of electrochemical enzymatic biosensor development is the immobilization of the enzymes, as it directly influences the sensitivity of the bioelectrode. Among the different methods used to incorporate enzymes on the surface of the transducers, layer-by-layer (LbL) self-assembly based on electrostatic interaction with polyelectrolytes of opposite charge stands out due to its simplicity and reproducibility. Aims: The aim of the work was to develop an electrochemical glucose biosensor by LbL assembly of a new functionalized chitosan polycation and the enzyme glucose oxidase (GOx). Methods: Chitosan was chemically functionalized with glucose by the Maillard reaction. The resulting polycation, named G-Chit, is soluble in the medium compatible with the enzyme. The bioelectrode was obtained by alternating adsorption of G-Chit and GOx onto carbon paste electrodes. By selecting the number of bilayer of G-Chit/GOX, the enzyme concentration, and the pH, the electroanalytical performance of the biosensor was optimized. The electrochemical responses were characterized by cyclic voltammetry and chronoamperometry. Results: Under optimized experimental conditions, the biosensor exhibited a sensitivity of (0.81 ± 0.03) µA mM-1 in a glucose concentration range of (0.18 to 1.75) mM. Discussion: Results indicated that catalytic response increases both with the number of G-Chit/GOx bilayers and the enzyme concentration, obtaining the best responses for 3 bilayers and 2 mg mL-1, respectively, while the optimum working pH value was 7.0. Conclusions: The analytical response of the biosensor was tested in milk samples with negligible matrix effects, suggesting a potential application in other dairy products. Results show that G-Chit appears promising for the immobilization of enzymes.

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An amperometric glucose biosensor based on electrostatic force induced layer-by-layer GOD/chitosan/pyrite on a glassy carbon electrode

Cited by 7 publications

References 61 publications

Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

A Noninvasive Sweat Glucose Biosensor Based on Glucose Oxidase/Multiwalled Carbon Nanotubes/Ferrocene-Polyaniline Film/Cu Electrodes

Self-Assembled Multilayers of Water Glucose Modified-Chitosan and Glucose Oxidase for Detection of Glucose in Milk Samples

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