An Amperometric Detector with a Platinum Tubular Electrode for High Performance Liquid Chromatography

Abstract

An amperometric detector based on a platinum tubular electrode was constructed from a long narrow-bored platinum tube which was directly connected to the column outlet. The tube is immersed in a vessel containing an electrolyte solution in which a common reference and counter electrodes are also immersed. Only the internal platinum wall is exposed to the electrolyte solution. At this geometrical arrangement, the platinum tube internal wall is nonuniformly polarized so that only a certain length of the tube from its outlet attains a potential suitable for the detection. As a result, the detector effective volume is substantially lower than the platinum tube total geometric volume. The detector was tested both in conventional and micro-HPLC systems.

Electroanalysis of Nitro and Amino Derivatives of Polycyclic Aromatic Hydrocarbons

“…and potassium chloride (aq.) -acetonitrile [152,210] GCE Phosphate buffer (aq.) -methanol [211] CFE Imidazole (aq.)…”

“…[209] BDDFE CV, DPV BR buffer (aq.) [199] PtE [152,210] CFE Imidazole (aq.) and potassium chloride (aq.)…”

“…Very good results were obtained using carbon-fiber detector [216], platinum tubular detector [210], wall-jet detector with glassy carbon paste working electrode [214], or thin-layer detector with boron-doped diamond film working electrode [212] currently used in our UNESCO Laboratory of Environmental Electrochemistry (see Table 2). …”

“…Platinum tubular detector constructed from a long narrow-bored platinum tube, which was directly connected to the column outlet, gives the response linearly dependent on the concentration of 1-aminonaphthalene (35) in the concentration range of 3×10 -8 -2×10 -5 mol L -1 with very low noise around 100 pA [210].…”

Modern Electrochemical Methods for Monitoring of Chemical Carcinogens

“…This fact can be demonstrated by the following three examples from our laboratory: Application of platinum tubular detector developed in our laboratory [31] for the determination of trace amounts of carcinogenic aromatic amines and its metabolites. This very simple device was used for HPLC ED determination of submicromolar concentrations of 1- and 2-aminonaphthalene [32], 1-,2-, 3- and 4-hydroxyphenanthrene [33] 3-, 5-, 6-, and 8-aminoquinoline [34], and 2- and 4-aminobiphenyl and 2- and 4-hydroxybiphenyl [35].…”