An amperometric biosensor for L-glutamate determination prepared from L-glutamate oxidase immobilized in polypyrrole-polyvinylsulphonate film

Abstract

In this paper, a novel amperometric L-glutamate (Glu) biosensor with immobilization of L-glutamate oxidase (L-GlOx) on polypyrrole-polyvinylsulphonate (PPy-PVS) film has been successfully developed. L-GlOx enzyme was immobilized on PPy-PVS film by cross-linking with glutaraldehyde (GA) and bovine serum albumin (BSA). Determination of Glu was carried out by oxidation of enzymatically produced H2O2 at 0.3 V versus Ag/AgCl. The optimum pH and temperature parameters were found to be 9.0 and 55 °C, respectively. There were three linear parts in the regions between 1.0 × 10(-9) and 1.0 × 10(-8) M (R(2) = 0.847), 5.0 × 10(-8) and 5.0 × 10(-7) M (R(2) = 0.997), 5.0 × 10(-7) and 5.0 × 10(-5) M (R(2) = 0.994). Storage stability, operation stability of the enzyme electrode were also studied.

Electrochemical study of the effect of radiofrequency on glutamate oxidase activity using a glutamate oxidase-based biosensor

“…6 . These results show that the native enzyme had the best performance at pH 7.4 which is consistent with many references [ 15 , 32 ]. Then, to study the effect of electromagnetic waves on the PE/Chit/GluOx irr responses, the optimal pHs of biosensor performance at different frequencies were investigated.…”

“…GluOx as one of the redox enzymes converts glutamate to NH 3 and α-ketoglutarate in the presence of O 2 and H 2 O 2 [ [14] , [15] , [16] ]. It should note that GluOx plays an important role in the function of GluOx-based biosensors, its catalytic activity is affected by environmental conditions.…”

“…It should note that GluOx plays an important role in the function of GluOx-based biosensors, its catalytic activity is affected by environmental conditions. So far, the effect of temperature, pH, buffer, and electrode type on the performance of biosensors has been studied and reported [ 15 ]. But the effect of electromagnetic waves on the GluOx, which in turn has a significant effect on the performance of GluOx-based biosensors, has not been studied.…”

Validated multi‐step approach for in vivo recording and analysis of optogenetically evoked glutamate in the mouse globus pallidus

“…Temperature changes on the electrode surface lead to fluctuations in the activity and sensitivity of the GluOx enzyme, which could contribute to this effect (Şimşek et al . ).…”

An Arrayed Micro‐glutamate Sensor Probe Integrated with On‐probe Ag/AgCl Reference and Counter Electrodes

“…Although GlutOx‐based electrodes for sensing purpose have been reported to have several advantages, such as simple fabrication process , fast detection time/near real‐time monitoring , long storage stability , and no requirement of cofactors , there is still room for improvement when attempting to apply these sensors for the purpose of in vivo tests. For example, approaches for improving sensor sensitivity and detection limit need to be investigated in order to track extracellular glutamate levels.…”