An Amperometric Biosensor Based on the Coimmobilization of Horseradish Peroxidase and Methylene Blue on a Carbon Nanotubes Modified Electrode

Xu, Jinzhong; Zhu, Jun‐Jie; Wu, Qiang; Hu, Zheng; Chen, Hong‐Yuan

doi:10.1002/elan.200390027

Cited by 207 publications

(77 citation statements)

References 32 publications

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“…This is expected, as MB is protonated upon reduction. Previous work with enzymemediated electrocatalytic cascades has demonstrated a nonlinear response to pH changes due to the pH dependence of the enzymatic activity (34)(35)(36)(37)(38)(39)(40)(41)(42). In these cases, where catalysis is limited by enzyme activity, constraints are imposed not only on the pH but also on other running conditions relevant to enzyme activity (34)(35)(36)(37)(38)(39)(40)(41)(42).…”

Section: Resultsmentioning

confidence: 99%

“…Enzymes such as horseradish peroxide and glucose oxidase have been shown to readily interact electronically with phenothiazine dyes, such as MB, and have had wide application as recognition elements for molecular sensing (33)(34)(35)(36)(37)(38)(39)(40)(41)(42). Detection platforms based on these enzymes are desirable due to their inherent catalytic activity.…”

mentioning

confidence: 99%

“…Detection platforms based on these enzymes are desirable due to their inherent catalytic activity. The application of a protein as an electron sink is also appealing because the protein shell itself can shield the redox core from the electrode surface (34)(35)(36)(37)(38)(39)(40)(41). Hemoglobin exhibits this property and has been shown to couple with Nile blue (42).…”

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confidence: 99%

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DNA sensing by electrocatalysis with hemoglobin

Pheeney

Guerra

Barton

2012

Proc. Natl. Acad. Sci. U.S.A.

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Electrocatalysis offers a means of electrochemical signal amplification, yet in DNA-based sensors, electrocatalysis has required highdensity DNA films and strict assembly and passivation conditions. Here, we describe the use of hemoglobin as a robust and effective electron sink for electrocatalysis in DNA sensing on low-density DNA films. Protein shielding of the heme redox center minimizes direct reduction at the electrode surface and permits assays on low-density DNA films. Electrocatalysis with methylene blue that is covalently tethered to the DNA by a flexible alkyl chain linkage allows for efficient interactions with both the base stack and hemoglobin. Consistent suppression of the redox signal upon incorporation of a single cytosine-adenine (CA) mismatch in the DNA oligomer demonstrates that both the unamplified and the electrocatalytically amplified redox signals are generated through DNAmediated charge transport. Electrocatalysis with hemoglobin is robust: It is stable to pH and temperature variations. The utility and applicability of electrocatalysis with hemoglobin is demonstrated through restriction enzyme detection, and an enhancement in sensitivity permits femtomole DNA sampling.DNA charge transport | DNA sensors | mismatch detection

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Section: Resultsmentioning

confidence: 99%

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DNA sensing by electrocatalysis with hemoglobin

Pheeney

Guerra

Barton

2012

Proc. Natl. Acad. Sci. U.S.A.

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“…The modified electrode showed a good bioelectrocatalytic activity toward H2O2 reduction. 136 By adding electron transfer mediator into solution, Davis et al developed glucose biosensor with GOx nonspecifically adsorbed on the SWNTs. 134 In addition, electrochemical biosensors based on dehydrogenase have been developed through electrocatalytic oxidation of NAD(P)H cofactors onto the CNTs or functionalized CNTs.…”

Section: ·2 Cnt-based Biosensorsmentioning

confidence: 99%

Electrochemistry and Electroanalytical Applications of Carbon Nanotubes: A Review

et al. 2005

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Carbon nanotubes (CNTs) have been proved to be a novel type of nanostructure with unique structural, electronic and mechanical properties and have drawn extensive attention since their discovery. [1][2][3] Research over the past decade has revealed that the CNTs constitute a new form of carbon materials that are finding striking applications in many fields, such as energy conversion and storage, 4,5 electromechanical actuators, 6,7 chemical sensing 8 and so forth. Generally, the nanotubes can be divided into two categories: single-walled carbon nanotubes (SWNTs) and multi-walled carbon nanotubes (MWNTs). The former can be regarded as a graphene cylinder formed by rolling seamlessly a single graphene sheet along an (m,n) lattice vector in the sheet. The (m,n) indices is a central parameter governing the metallicity and chirality of the tubes. The latter is composed of coaxial multilayer graphene tubes with interlayer space of 0.34 nm. The diameter varies from 0.4 nm to 3 nm for the SWNTs and from 1.4 nm to 100 nm for the MWNTs (typical transmission electron microscopic images of the CNTs are shown in Fig. 1).Due to the unique physical and chemical properties and thus the striking applications in various research and industrial fields, the CNTs have drawn extensive interest over the past decade. To date, we have witnessed great successes in the synthesis of the CNTs and in understanding of their chemical and physical properties. Several excellent reviews concerning these issues have appeared in the literature. 9,10 Currently, increasing interests are being focused on the construction of the CNT-based functional nanodevices with novel properties for practical applications.On the other hand, the CNTs represent a new kind of carbonbased materials and are superior to other kinds of carbon materials commonly used in electrochemistry, such as glassy carbon (GC), graphite and diamond, mainly in the special structural features and unique electronic properties. As a result, besides their striking applications in other fields, study of the CNT electrochemistry thus far has revealed that these unique properties of the CNTs substantially make them useful for electrochemical investigations, e.g., electrocatalysis, direct electrochemistry of proteins and electroanalytical applications such as electrochemical sensors and biosensors. 11,12 The electroanalytical applications of the CNTs for construction of This review addresses recent developments in electrochemistry and electroanalytical chemistry of carbon nanotubes (CNTs). CNTs have been proved to possess unique electronic, chemical and structural features that make them very attractive for electrochemical studies and electrochemical applications. For example, the structural and electronic properties of the CNTs endow them with distinct electrocatalytic activities and capabilities for facilitating direct electrochemistry of proteins and enzymes from other kinds of carbon materials. These striking electrochemical properties of the CNTs pave the way to CNT-based bioelectrochemist...

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“…Several numbers of MWCNTs modified amperometric peroxide biosensors have been fabricated based on MWCNT/thionine/Nafion modified PIGE [24], Carbon nanotube/Chitosan/HRP/SG modified GCE [25], MWCNT/HRP/gold modified electrode [26], poly(TB)/ HRP/MWCNT/CHIT modified electrode [27], MWCNT/ BSA/HRP/Ferrocene modified electrode [28], MWCNT/ HRP/Methylene blue modified electrode [29] and MWCNT/TTF-TCNQ/HRP modified gold electrode [30].…”

Section: Introductionmentioning

confidence: 99%

Analytical Biosensing of Hydrogen Peroxide on Brilliant Cresyl Blue/Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrode

Peng¹,

Upadhyay²,

Chen³

2010

Electroanalysis

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A cationic quinine-imide dye brilliant cresyl blue (BCB) and horseradish peroxidase (HRP) were co-immobilized within ormosil on multiwalled carbon nanotubes modified glassy carbon electrode for the fabrication of highly sensitive and selective hydrogen peroxide biosensor. The presence of epoxy group in ormosil as organic moiety improves the mechanical strength and transparency of the film and amino group provides biocompatible microenvironment for the immobilization of enzyme. The presence of MWCNTs improved the conductivity of the nanocomposite film. The surface characterization of MWCNT modified ormosil nanocomposite film was performed with scanning electron microscopy (SEM) and atomic force microscopy (AFM). Cyclic voltammetry and amperometry measurements were used to study and optimize the performance of the resulting peroxide biosensor. The apparent Michaelis -Menten constant was determined to be 1.5 mM. The proposed H 2 O 2 biosensor exhibited wide linear range from 3 Â 10 À7 to 1 Â 10 À4 M, and low detection limit 1 Â 10 À7 M (S/N ¼ 3) with fast response time < 5 s. The probable interferences in bio-matrix were selected to test the selectivity and no significant response was observed in the biosensor. This biosensor possessed good analytical performance and long term storage stability.

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An Amperometric Biosensor Based on the Coimmobilization of Horseradish Peroxidase and Methylene Blue on a Carbon Nanotubes Modified Electrode

Cited by 207 publications

References 32 publications

DNA sensing by electrocatalysis with hemoglobin

DNA sensing by electrocatalysis with hemoglobin

Electrochemistry and Electroanalytical Applications of Carbon Nanotubes: A Review

Analytical Biosensing of Hydrogen Peroxide on Brilliant Cresyl Blue/Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrode

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