An aminotransferase from bacterium ATCC 55552 deaminates hydrolyzed fumonisin B1

Heinl, Stefan; Hartinger, Doris; Thamhesl, Michaela; Schatzmayr, Gerd; Moll, Wulf-Dieter; Grabherr, Reingard

doi:10.1007/s10532-010-9371-y

Cited by 32 publications

(23 citation statements)

References 14 publications

(12 reference statements)

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“…Previous research identified degradation of FB1 by microorganisms is likely composed of two essential steps: hydrolysis to hydrolyzed fumonisin B1 (HFB1) and deamination of the hydrolysis product (Heinl et al, 2011). Benedetti et al (Benedetti et al, 2006) demonstrated that different bacterial isolates of maize ecosystem have the ability to degrade FB1, among of which, Sphingomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

Fu¹,

Feng²,

YaNan³

et al. 2015

IJAB

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Corn ear rot has been the most important disease of the world. It is mainly caused by Fusarium moniliforme which produce a number of mycotoxins including Fumonisin B1 (FB1). Aiming at supplying a potentially effective biological control agent against F. moniliforme, Bacillus megaterium MHT6 isolated from China, was tested for its antagonistic activity against F. moniliforme. Moreover, both antifungal activity and the active compound of strain MHT6 were tested and identified. In order to improve its antifungal activity, a random mutagenesis generated using He-Ne laser irradiation was applied on B. megaterium MHT6. After the mutation, one hundred bacterial colonies were obtained and their effects against F. moniliforme were assessed in a plate assay. Eight colonies (8%) exhibited higher inhibition activity as compared to the wild type MHT6. Among them, the mutant MHT 88 which showed the strongest antagonistic ability was selected out. Through bacterial motility assays, the mutant MHT88 showed stronger swarming motility than wild type MHT6. According to FPLC and MALDI-TOF-MS, the production of two main antagonistic lipopeptides such as surfactin and fengycin increased in mutant MHT88. Especially the surfactin, which can't be detected in the wild strain MHT6, was obviously observed in mutant. Moreover, the cell-free supernatant of the mutant MHT88 showed stronger capability in degrading FB1 compared to MHT6. This study provided a promising biological agent to inhibit F. moniliforme and suppress corn ear rot disease.

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Section: Discussionmentioning

confidence: 99%

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

Fu¹,

Feng²,

YaNan³

et al. 2015

IJAB

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“…MTA144, isolated from composted earth [92]. In this strain the two key DEs were identified within a gene cluster: fumD , encoding a type B carboxylesterase, and fumI , encoding an aminotransferase [80,93,94]. The authors also demonstrated the degrading activities of the two recombinant enzymes by in vitro assays.…”

Section: Mycotoxin Biotransformation By Enzymesmentioning

confidence: 99%

Mycotoxin Biotransformation by Native and Commercial Enzymes: Present and Future Perspectives

Loi

Fanelli

Liuzzi

et al. 2017

Toxins

167

131

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Worldwide mycotoxins contamination has a significant impact on animal and human health, and leads to economic losses accounted for billions of dollars annually. Since the application of pre- and post- harvest strategies, including chemical or physical removal, are not sufficiently effective, biological transformation is considered the most promising yet challenging approach to reduce mycotoxins accumulation. Although several microorganisms were reported to degrade mycotoxins, only a few enzymes have been identified, purified and characterized for this activity. This review focuses on the biotransformation of mycotoxins performed with purified enzymes isolated from bacteria, fungi and plants, whose activity was validated in in vitro and in vivo assays, including patented ones and commercial preparations. Furthermore, we will present some applications for detoxifying enzymes in food, feed, biogas and biofuel industries, describing their limitation and potentialities.

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“…For example, fumonisin biodegradation was observed through the elimination of the tricarballylate side chains and amino groups. The enzymatic hydrolysis of such mycotoxins might involve carboxylesterases and aminotransferases from bacteria such as Sphingomonas and Sphingopyxis normally found in soil, which have the ability to detoxify recalcitrant persistent organic pollutants (PoPs) such as polycyclic aromatic hydrocarbons (PAHs) [40][41][42][43]. Other researchers have reported degradation or detoxiication of fumonisin, including by-products, by oxidative deaminase from Exophiala sp., a common soil organism [42][43][44].…”

Section: Biological Reduction Of Mycotoxinsmentioning

confidence: 99%

Leaching of Cyanogens and Mycotoxins from Cultivated Cassava into Agricultural Soil: Effects on Groundwater Quality

Itoba-Tombo¹,

Ntwampe²,

Mudumbi³

2017

Aflatoxin-Control, Analysis, Detection and Health Risks

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Cyanogens and mycotoxins are vital in protecting lora against predation. Nevertheless, their increased concentrations and by-products in agricultural soil could result in produce contamination and decreased crop yield and soil productivity. When exposed to unsuitable weather conditions, agricultural produce such as cassava is susceptible to bacterial and fungal atack, culminating in spoilage, particularly in arid and semi-arid regions, and contributing to cyanogen and mycotoxins loading of the arable land. The movement of cyanogen including mycotoxins in such soil can result in sub-surface and/ or groundwater contamination, thus deteriorating the soil's environmental health and negatively afecting wildlife and humans. Persistent cyanogen and mycotoxins loading into agricultural soil changes its physico-chemical characteristics and biotic parameters. These contaminants and their biodegradation by-products can be dispersed from soil's surface and sub-surface to groundwater systems by permeation and percolation through the upper soil layer into underground water reservoirs, which can result in their exposure to humans and wildlife. Thus, an assessment and monitoring of cyanogen and mycotoxins loading impacts on arable land and groundwater in communities with minimal resources should be done. Overall, these toxicants impacts on agricultural soil's biotic community, afect soil's aggregates, functionality and lead to the soil's low productivity, cross-contamination of fresh agricultural produce.

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An aminotransferase from bacterium ATCC 55552 deaminates hydrolyzed fumonisin B1

Cited by 32 publications

References 14 publications

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

Mycotoxin Biotransformation by Native and Commercial Enzymes: Present and Future Perspectives

Leaching of Cyanogens and Mycotoxins from Cultivated Cassava into Agricultural Soil: Effects on Groundwater Quality

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