An alternative route to dye–polymer complexation study using asymmetrical flow field-flow fractionation

Boye, Susanne; Polikarpov, Nikita; Appelhans, Dietmar; Lederer, Albena

doi:10.1016/j.chroma.2010.05.036

Cited by 28 publications

(27 citation statements)

References 37 publications

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“…This is possible because the light scattering intensity is in direct relation with the molar mass. In this regard, the combination of AF4 or SEC with scattering techniques (see Figure 16) is a powerful tool for comprehensive characterisation of a wide range of polymers [194][195][196][197]. .…”

Section: Determination Principle and Requirementsmentioning

confidence: 99%

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

et al. 2018

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Abstract:Casein is the major protein fraction in milk, and its cross-linking has been a topic of scientific interest for many years. Enzymatic cross-linking has huge potential to modify relevant techno-functional properties of casein, whereas non-enzymatic cross-linking occurs naturally during the storage and processing of milk and dairy products. Two size separation techniques were applied for characterisation of these reactions: gel electrophoresis and size exclusion chromatography. This review summarises their separation principles and discusses the outcome of studies on cross-linked casein from the last~20 years. Both methods, however, show limitations concerning separation range and are applied mainly under denaturing and reducing conditions. In contrast, field flow fractionation has a broad separation range and can be easily applied under native conditions. Although this method has become a powerful tool in polymer and nanoparticle analysis and was used in few studies on casein micelles, it has not yet been applied to investigate cross-linked casein. Finally, the principles and requirements for absolute molar mass determination are reviewed, which will be of increased interest in the future since suitable calibration substances for casein polymers are scarce.

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Section: Determination Principle and Requirementsmentioning

confidence: 99%

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

et al. 2018

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“…The results of ultrafiltration experiments were confirmed by AF4, an elution‐based chromatography‐like separation method commonly applied for the characterization of nanoparticles, polymers, and proteins. The advantages of AF4 include a broad separation range accessible by a single channel and significantly reduced risk of shear degradation . The application of this method constitutes a significant extension of our previous studies on the complexation patterns of glycosylated branched polymers .…”

Section: Zeta Potential Of Ppi Dendrimers and Nucleotide–dendrimer Comentioning

confidence: 93%

Effect of the Structure of Therapeutic Adenosine Analogues on Stability and Surface Electrostatic Potential of their Complexes with Poly(propyleneimine) Dendrimers

Gorzkiewicz

Appelhans

Boye

et al. 2019

Macromol. Rapid Commun.

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Poly(propyleneimine) glycodendrimers are proposed as nanocarriers for triphosphate forms of anticancer adenosine analogues to improve the efficiency of chemotherapy and to overcome drug resistance mechanisms. This approach has proven successful for fludarabine administration—an autonomous way of cellular entry of a nucleotide–dendrimer noncovalent complex enables an increase in the intracellular accumulation and cytotoxic activity of the active metabolite of the drug. However, the attempt to apply an analogous strategy for clofarabine results in the inhibition of drug activity. To better understand this phenomenon, characterization and comparison of drug‐dendrimer complexes were needed to indicate the differences in their surface properties and the strengths of fludarabine‐dendrimer and clofarabine‐dendrimer interactions. Here, zeta potential measurements, ultrafiltration, and asymmetric flow field‐flow fractionation are applied to determine the surface electrostatic potential and stability of nucleotide–dendrimer formulations. This approach significantly extends the authors’ research on the complexation potential of perfectly branched macromolecules, ultimately explaining previously observed differences and their consequences.

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“…Recently, our studies showed that cationic poly(propylene imine) dendrimers and hyperbranched poly(ethylene imine)s (PEI) decorated with oligosaccharide shells are suitable materials to complex anionic analyte molecules (e.g., ATP,13, 14 Mant‐ATP,15 Rose Bengal16 and nucleic acids17, 18). Physico‐chemical and molecular modelling studies confirmed the core–shell architectures of these dendritic glycopolymers 19.…”

Section: Number Of Each Type Of Analyte Molecule Complexed Per Macrommentioning

confidence: 99%

“…In previous studies, these PEI‐OS macromolecules were extensively investigated by several analytical methods to learn about their chemical composition, globular molecular shape and pH‐dependent cationic charge density 13. 16, 17 Moreover, the PEI‐OS macromolecules preferentially exist as isolated species in aqueous solution and do not agglomerate 13. 19 Typical isoelectric points for the PEI‐OS are between pH 7.2 and 9.6 (see the Supporting Information, Table S2), whereas the parent hyperbranched PEI ( PEI‐5 k ) has an isoelectric point of pH 10.5 16.…”

Section: Number Of Each Type Of Analyte Molecule Complexed Per Macrommentioning

confidence: 99%

“…16, 17 Moreover, the PEI‐OS macromolecules preferentially exist as isolated species in aqueous solution and do not agglomerate 13. 19 Typical isoelectric points for the PEI‐OS are between pH 7.2 and 9.6 (see the Supporting Information, Table S2), whereas the parent hyperbranched PEI ( PEI‐5 k ) has an isoelectric point of pH 10.5 16. The PEI‐OS species all show increasing cationic charge density with decreasing pH 13.…”

Section: Number Of Each Type Of Analyte Molecule Complexed Per Macrommentioning

confidence: 99%

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Oligosaccharide Shells as a Decisive Factor for Moderate and Strong Ionic Interactions of Dendritic Poly(ethylene imine) Scaffolds under Shear Forces

Tripp

Appelhans

Striegler

et al. 2014

Chemistry A European J

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For better understanding and improving the non-covalent interactions of dendritic core-shell, we evaluated the interactions of hyperbranched poly(ethylene imine) (PEI) decorated with various oligosaccharide shells with water-soluble B vitamins, an estradiol derivative and pantoprazole. Depending on the different properties of the analyte molecules, dendritic core-shell glyco architectures showed (very) weak, moderate and strong interactions with the analyte molecules. Thus, ionic interactions are the strongest driving force for the formation of host-guest complexes. The core-shell glyco architecture is a necessary prerequisite for stable analyte/PEI complexes; the pure hyperbranched PEI did not show any sufficiently strong interactions with neutral, cationic or anionic analytes under the shear forces applied during ultrafiltration of pure aqueous solution without an adjusted pH. Thus, only robust non-covalent interactions between analytes and the dendritic polyamine scaffold of the glycopolymer structure survive this separation step and allow isolation of stable host-guest complexes in aqueous solution.

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An alternative route to dye–polymer complexation study using asymmetrical flow field-flow fractionation

Cited by 28 publications

References 37 publications

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

Effect of the Structure of Therapeutic Adenosine Analogues on Stability and Surface Electrostatic Potential of their Complexes with Poly(propyleneimine) Dendrimers

Oligosaccharide Shells as a Decisive Factor for Moderate and Strong Ionic Interactions of Dendritic Poly(ethylene imine) Scaffolds under Shear Forces

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