An alternative regulatory pathway of the acute phase response: the role of fibroblast-derived interferon-β 2

Koj, Aleksander; Ah, Gordon; Gauldie, Jack

doi:10.1007/bf01960222

Cited by 15 publications

(9 citation statements)

References 15 publications

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“…HepG2 cells spontaneously produce fibrinogen (Gauldie et al, 1987;Koj et al, 1988). After treatment with rhIL-6in a time-and concentration-dependent manner -the fibrinogen production of the cells increases to 280-350% of the level of untreated cells (Gauldie et al, 1987;Koj et al, 1988;Heinrich et al, 1990).…”

Section: Effect Of Peptides On Fibrinogen Production In Hepg2 Cellsmentioning

confidence: 99%

“…Based on their influence on spontaneous fibrinogen secretion (Gauldie et al, 1987;Koj et al, 1988), peptides can be classified as stimulatory or inhibitory compounds. Based on their influence on spontaneous fibrinogen secretion (Gauldie et al, 1987;Koj et al, 1988), peptides can be classified as stimulatory or inhibitory compounds.…”

Section: Effect Of Peptides On Fibrinogen Production In Hepg2 Cellsmentioning

confidence: 99%

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Interleukin-6 N-Terminal Peptides Modulate the Expression of junB Protooncogene and the Production of Fibrinogen in HepG2 Cells

Bősze

Hudecz

Igaz

et al. 2003

Biological Chemistry

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Interleukin-6 (IL-6) is a helical cytokine exerting pleiotropic activities including the regulation of hematopoiesis, B cell activation and acute-phase reaction. The structure-function relationship of the molecule is the subject of intensive investigation using point and deletion mutants. Our objective was to analyse the role of the N-terminal 18-46 region in IL-6-mediated expression of junB protooncogene and fibrinogen production, reflecting the acute phase response, with synthetic overlapping peptides. mRNA expression of junB was monitored by competitive RT-PCR, while sandwich ELISA was used for the detection of fibrinogen in the supernatant of HepG2 human hepatoma cells. We found that even short synthetic octapeptides can be stimulatory (in the absence of IL-6) or inhibitory (in the presence of IL-6) in both assays. To establish the molecular mechanism by which synthetic peptides exert their biological effects electromobility shift assay was carried out using HepG2 nuclear extracts. Peptides inducing junB expression initiate gel shifts of STAT3/DNA complexes, which may indicate the involvement of this signal transduction pathway. Circular dicroism spectroscopy data suggest that 8-11-mer peptides representing different parts of the 18-46 region have a marked tendency to adopt ordered conformations in a water/trifluoroethanol (1:1 v/v) mixture. Competition studies with rhIL-6 and selected fluorophore-labelled peptides indicate the presence of more than one binding site on soluble IL-6 receptor. Considering the possible multiple etiologic role of IL-6 in the pathogenesis of various diseases, these peptides could be useful for dissection of IL-6 related biological effects.

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Section: Effect Of Peptides On Fibrinogen Production In Hepg2 Cellsmentioning

confidence: 99%

Section: Effect Of Peptides On Fibrinogen Production In Hepg2 Cellsmentioning

confidence: 99%

Interleukin-6 N-Terminal Peptides Modulate the Expression of junB Protooncogene and the Production of Fibrinogen in HepG2 Cells

Bősze

Hudecz

Igaz

et al. 2003

Biological Chemistry

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“…A protein secreted by human fibroblasts, interferonbeta,. strongly stimulates an acute phase response in cultured liver cells [74]. It is possible that different monokines will affect synthesis of different components of the acute phase response or modify the activity of certain acute phase proteins by post-translational modifications such as glycosylation [75].…”

Section: Dietary Fish Oil and Acute Phase Reactantsmentioning

confidence: 99%

Dietary n‐3 fatty acids and arthritis

Cathcart

Gonnerman

Leslie

et al. 1989

Journal of Internal Medicine

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Abstract. We have evidence that dietary fish oil (FO) decreases severity of collagen‐induced arthritis (CIA), changes the fatty acid composition of macrophage (Mϕ) membrane phospholipids, decreases Mϕ synthesis of prostaglandins (PGs), changes chemotactic ability of Mϕs, and affects metabolism of acute phase proteins. Gender also has pronounced effects on susceptibility to CIA and Mϕ prostaglandin profiles. The mechanisms by which dietary n‐3 fatty acids may act to alleviate symptoms of CIA, as well as interactions of dietary n‐3 and n‐6 fatty acids and gender are discussed. We suggest that the ability of FO diets to influence favourably the course of chronic inflammatory diseases is mediated via alterations in n‐6 fatty acid metabolism and that intrinsic differences in n‐6 fatty acid metabolism may account not only for our reported gender differences in incidence and severity of CIA, but also the well‐documented sexual dimorphism in immune/inflammatory responses in general.

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“…Cultured human hepatocytes should be suitable for performing such studies because FIB is synthesized exclusively by hepatocytes and Bz mainly acts on the liver [14]. Moreover, HepG2 cells constitute a permanent human cell line that is a well-established, versatile model for studying problems related to human liver [15][16][17]. HepG2 cells were isolated by Aden et al [18] and have been shown to secrete constitutively most, but not all, plasma proteins [19].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, they synthesize and secrete a 1 -fetoprotein (AFP), apolipoproteins such as apo A-I and apo B and C-reactive protein [19,20], but not the vitamin D-binding (Gc) protein or apo (a) [21]. Protein synthesis by HepG2 cells depends on the composition of the culture medium [22]; more importantly, it is modulated by various cytokines, steroids and hormones [15][16][17]. In particular, IL-6, which cannot be synthesized by HepG2 cells [23], strikingly modifies the synthesis of acute-phase proteins (APP) such as FIB and AFP [11].…”

Section: Introductionmentioning

confidence: 99%

Bezafibrate down‐regulates fibrinogen biosynthesis in human hepatoma HepG2 cells

Binsack¹,

Stegmeier²,

Dörge³

et al. 1998

Eur J Clin Investigation

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An alternative regulatory pathway of the acute phase response: the role of fibroblast-derived interferon-β 2

Cited by 15 publications

References 15 publications

Interleukin-6 N-Terminal Peptides Modulate the Expression of junB Protooncogene and the Production of Fibrinogen in HepG2 Cells

Interleukin-6 N-Terminal Peptides Modulate the Expression of junB Protooncogene and the Production of Fibrinogen in HepG2 Cells

Dietary n‐3 fatty acids and arthritis

Bezafibrate down‐regulates fibrinogen biosynthesis in human hepatoma HepG2 cells

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