An alternative mini buffy coat preparation method for adult patients with extracorporeal photopheresis contraindications

Grabmer, Christoph; Schlager, Sandra; Mayer, G; Streif, Doris; Lener, Thomas; Schallmoser, Katharina; Rohde, Eva

doi:10.1002/jca.21455

Cited by 5 publications

(10 citation statements)

References 19 publications

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“…Their technique resulted in similar WBC yields and higher lymphocyte yields than the Compomat device using method, but since it requires minimum processing blood volume of 300 mL its use is limited for the adult patients. The recovery of WBC and MNC after separation on the Sepax were 88.3 ± 9.4%, and 90.8 ± 11.4%, respectively, which were comparable to the Compomat technique …”

Section: Discussionsupporting

confidence: 52%

“…Hackstein et al prepared the buffy coat for pediatric patients using fully automated separator device Compomat G4 (Fresenius, Bad Homburg, Germany) . Recently, Gramber et al developed an alternative mini buffy coat preparation method for adult patients using the Spectra Optia Apheresis System (Terumo BCT) . Their technique resulted in similar WBC yields and higher lymphocyte yields than the Compomat device using method, but since it requires minimum processing blood volume of 300 mL its use is limited for the adult patients.…”

Section: Discussionmentioning

confidence: 99%

“…The recovery of WBC and MNC after separation on the Sepax were 88.3 ± 9.4%, and 90.8 ± 11.4%, respectively, which were comparable to the Compomat technique. 7 Various authors have assumed an impact of collected and treated cell numbers on patient's outcomes. Compared with the apheresis ECP methods, the lowest cell numbers are collected and treated in mini-ECP procedures.…”

Section: Discussionmentioning

confidence: 99%

“…Mini‐ECP is an “off line” technique that has been developed for use in the treatment of small children and patients with apheresis contraindications. In mini‐ECP the whole blood is collected from the patients and various methods have been used for mononuclear cell (MNC) separation and buffy coat preparation . In this report we describe an alternative method for mini buffy coat preparation using the automated Sepax cell laboratory separator (Biosafe, Switzerland) for the “off line” technique ECP treatment of a low body weight child with graft‐vs‐host‐disease.…”

Section: Introductionmentioning

confidence: 99%

“…In mini-ECP the whole blood is collected from the patients and various methods have been used for mononuclear cell (MNC) separation and buffy coat preparation. [5][6][7] In this report we describe an alternative method for mini buffy coat preparation using the automated Sepax cell laboratory separator (Biosafe, Switzerland) for the "off line" technique ECP treatment of a low body weight child with graft-vs-host-disease. The "UCB protocol" designed for isolation of the buffy-coat fraction from umbilical cord blood was used.…”

Section: Introductionmentioning

confidence: 99%

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An automated mini buffy coat preparation method for use in mini extracorporeal photopheresis treatment of graft‐vs‐host‐disease in a low body weight pediatric patient

Matić

Bojanić

Mazić

et al. 2019

J of Clinical Apheresis

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A mini extracorporeal photopheresis (mini‐ECP) “off line” technique has been developed for use in the treatment of small children and patients with apheresis contraindications. Until now various methods have been used for buffy coat separation from whole blood. In this report we describe a protocol for mini buffy coat preparation using the automated Sepax laboratory separator for “off line” ECP treatment in a low body weight child with graft‐vs‐host‐disease. According to our results this alternative method has been proven feasible and tolerable.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An automated mini buffy coat preparation method for use in mini extracorporeal photopheresis treatment of graft‐vs‐host‐disease in a low body weight pediatric patient

Matić

Bojanić

Mazić

et al. 2019

J of Clinical Apheresis

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Increasing the collection flow rate to 2 mL/min is effective and reduces the procedure time in off‐line photopheresis

Mayer,

Mayr,

Koch

et al. 2023

Transfusion

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BackgroundExtracorporeal photopheresis (ECP) treatment, mostly based on apheresis technology, is used for immunomodulation in various diseases such as cutaneous T‐cell lymphoma, graft versus host disease and other (auto)immune disorders. The aim of this study was to collect high cell counts and purity in shorter procedure times using an ECP off‐line system with an increased collection flow rate of 2 mL/min to a target volume of 200 mL buffy coat.Study design and methodsIn this prospective study, data of routinely performed off‐line photopheresis treatments were collected and analyzed at the Central Institute for Blood Transfusion & Department of Immunology (ZIB) of the Tirol Kliniken, to assess absolute cell counts and procedure times and to calculate collection efficiencies (CE2).ResultsA total of 22 patients participated in this study. The processed blood volume was 4312 mL, the collection time 120 min, overall procedure time 157 min and the absolute cell counts of treated white blood cells (WBC) and mononuclear cells (MNC) were 5.0 and 4.3 × 109 respectively (median values). The calculated CE2 for WBC and MNC was 21.1% and 58.5%, the proportion of treated MNCs of the total number of MNCs present was 55.0%.ConclusionThe data presented in this study show high therapeutically effective cell counts collected with a high MNC purity within a shorter overall collection/procedure time due to an increased collection flow rate.

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An alternative for extracorporeal photopheresis: 8‐methoxypsoralen and UVA‐treated leucocytes from allogeneic donors improve graft‐versus‐host disease in mice

et al. 2018

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Background and Objective Extracorporeal photopheresis (ECP) is an important immune tolerance inducing therapy for graft-versus-host disease (GvHD). However, a sufficient number of ECP cycles cannot be performed in patients with severe GvHD and contraindications for apheresis. Allogeneic sources of leucocytes for use as ECP treatment would be of great benefit. Therefore, this study aimed to test the therapeutic potential of novel sources of leucocytes for ECP. Materials and MethodsGraft-versus-host disease mice were treated with ECP using therapeutic cells from different allogeneic sources. Splenocytes were incubated with 8-methoxypsoralen (8-MOP), irradiated with UVA light and injected into GvHD mice as a model for ECP. ResultsThe therapy with 8-MOP/UVA-treated cells from healthy mice of the bone marrow transplantation (BMT) donor strain reduced the GvHD symptoms, at least in a model of chronic GvHD. In the acute GvHD model, 8-MOP/UVA-treated cells from the BMT donor or recipient strain did not show significant improvements in GvHD symptoms or survival time. Pre-activation of cells by mixed lymphocyte reactions before 8-MOP/UVA treatment also failed to result in significant differences in survival time or GvHD score. In contrast, ECP with third-party 8-MOP/UVA-treated cells from a HLA-mismatched donor resulted in a mean survival time of 37 days compared to 21 days in the control group.Conclusion In our analysis of novel allogeneic leucocyte sources for ECP, we could demonstrate that the source of the 8-MOP/UVA-treated cells is crucial. The underlying immunologic effect of allogeneic 8-MOP/UVA-treated cells needs to be investigated in future studies.

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An alternative mini buffy coat preparation method for adult patients with extracorporeal photopheresis contraindications

Cited by 5 publications

References 19 publications

An automated mini buffy coat preparation method for use in mini extracorporeal photopheresis treatment of graft‐vs‐host‐disease in a low body weight pediatric patient

An automated mini buffy coat preparation method for use in mini extracorporeal photopheresis treatment of graft‐vs‐host‐disease in a low body weight pediatric patient

Increasing the collection flow rate to 2 mL/min is effective and reduces the procedure time in off‐line photopheresis

An alternative for extracorporeal photopheresis: 8‐methoxypsoralen and UVA‐treated leucocytes from allogeneic donors improve graft‐versus‐host disease in mice

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