An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples

Baptista, Leandra Santos; Amaral, Rui; Carias, Rosana Bizon Vieira; Aniceto, Marcelo; Claudio-da-Silva, César; Borojević, Radovan

doi:10.3109/14653240902981144

Cited by 97 publications

(94 citation statements)

References 21 publications

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“…Besides taking advantages in time and cost when using mechanic process, their reproducibility makes it a preferred method for larger volumes of samples. We observed a large standard derivation among cell numbers isolated with the enzymatic digestion process, in opposition to mechanic process (Baptista et al, 2009). However, the most advantage of this innovative process is the possibility of cryopreservation of freshly isolated MSC cells.…”

Section: Mesenchymal Stem Cell Isolationmentioning

confidence: 85%

“…We were able to isolate MSC from lipoaspirates harvested by standard or vibro-assisted techniques, using a mechanic method (see topic 4). The total number of MSC obtained from vibro-assisted lipoaspirate samples was superior to that obtained from the standard one, but no differences in adhesion or proliferation in culture was observed (Baptista et al, 2009). When taking into account the isolation of MSC with high cell quality, the use of a defined method of tissue harvesting and preparation in the operating room is crucial.…”

Section: Adipose Tissue Harvesting and Preparation For Isolating Mesementioning

confidence: 92%

“…They were also positive for CD34, which is reported only in adipose tissue-derived mesenchymal cells . They also were able to accumulate lipid droplets, deposit extracellular calcium and cartilage extracellular matrix, under specific stimuli for each differentiation event (Baptista et al, 2009). Their proprieties support their use for diverse therapeutic applications.…”

Section: Mesenchymal Stem Cell Isolationmentioning

confidence: 96%

“…Cells isolated by the mechanic method maintained the ability to proliferate and the fibroblastic morphology even after 10 passages. No stress actin bundles were visualized (Baptista et al, 2009). Fig.…”

Section: Mesenchymal Stem Cell Characterization For Quality Control 4mentioning

confidence: 99%

“…This enzymatic procedure generates tissue fragments that should be removed before cell plating through a 100-150 μm nylon mesh. Irrespective of the source of tissue, enzymatic digestion is time consuming and expensive, especially when applied to large volumes of tissue (Baptista et al, 2009); decreased cell viability due to lytic activity is also a problem with this method (Ishige et al, 2009). We have described a novel method of isolating MSC from lipoaspirate samples, based on mechanical tissue dissociation.…”

Section: Mesenchymal Stem Cell Isolationmentioning

confidence: 99%

See 4 more Smart Citations

Processing of Lipoaspirate Samples for Optimal Mesenchymal Stem Cells Isolation

Baptista¹,

Silva²,

Pedrosa³

et al. 2011

Advanced Techniques in Liposuction and Fat Transfer

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Section: Mesenchymal Stem Cell Isolationmentioning

confidence: 85%

Section: Adipose Tissue Harvesting and Preparation For Isolating Mesementioning

confidence: 92%

Section: Mesenchymal Stem Cell Isolationmentioning

confidence: 96%

Section: Mesenchymal Stem Cell Characterization For Quality Control 4mentioning

confidence: 99%

Section: Mesenchymal Stem Cell Isolationmentioning

confidence: 99%

See 3 more Smart Citations

Processing of Lipoaspirate Samples for Optimal Mesenchymal Stem Cells Isolation

Baptista¹,

Silva²,

Pedrosa³

et al. 2011

Advanced Techniques in Liposuction and Fat Transfer

Self Cite

View full text Add to dashboard Cite

A small population of resident limb bud mesenchymal cells express few MSC‐associated markers, but the expression of these markers is increased immediately after cell culture

Marín-Llera

Chimal‐Monroy

2018

Cell Biology International

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Skeletal progenitors are derived from resident limb bud mesenchymal cells of the vertebrate embryos. However, it remains poorly understood if they represent stem cells, progenitors, or multipotent mesenchymal stromal cells (MSC). Derived‐MSC of different adult tissues under in vitro experimental conditions can differentiate into the same cellular lineages that are present in the limb. Here, comparing non‐cultured versus cultured mesenchymal limb bud cells, we determined the expression of MSC‐associated markers, the in vitro differentiation capacity and their gene expression profile. Results showed that in freshly isolated limb bud mesenchymal cells, the proportion of cells expressing Sca1, CD44, CD105, CD90, and CD73 is very low and a low expression of lineage‐specific genes was observed. However, recently seeded limb bud mesenchymal cells acquired Sca1 and CD44 markers and the expression of the key differentiation genes Runx2 and Sox9, while Scx and Pparg genes decreased. Also, their chondrogenic differentiation capacity decreased through cellular passages while the osteogenic increased. Our findings suggest that the modification of the cell adhesion process through the in vitro method changed the limb mesenchymal cell immunophenotype leading to the expression and maintenance of common MSC‐associated markers. These findings could have a significant impact on MSC study and isolation strategy because they could explain common variations observed in the MSC immunophenotype in different tissues.

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Isolation of Ready‐to‐Use Adipose‐Derived Stem Cell (ASC) Pellet for Clinical Applications and a Comparative Overview of Alternate Methods for ASC Isolation

Raposio

Bertozzi

2017

CP Stem Cell Biology

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Current literature does not offer a standardized method to isolate adipose-derived stem cells (ASCs) for clinical applications and hence clinical studies using ASCs often show inconsistent results. Most of these studies borrow laboratory or benchside-derived protocols, which are complex, time consuming, and involve the use of chemical, animal-derived reagents. In this unit we describe a relatively simple and faster isolation protocol that allows collection of a ready-to-use ASC pellet for clinical application. All steps are performed in a closed circuit in order to guarantee maximum process sterility. Once the adipose tissue is harvested by means of a standard liposuction procedure, it undergoes a first centrifugation in order to remove the oil and serous fractions. Then ASCs are released by enzymatic digestion from the surrounding connective tissue scaffold. Finally a double series of washing and centrifugation allows one to obtain the ASC pellet alone. We usually graft this ASC pellet onto the skin edge and to the bottom of chronic skin ulcers as ASCs proved to be effective in promoting wound healing processes. Moreover, an increasing number of clinical studies are currently ongoing to test their potential in every medical field, from orthopedics to cardiology, oncology, autoimmune diseases, and tissue engineering. © 2017 by John Wiley & Sons, Inc.

show abstract

An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples

Cited by 97 publications

References 21 publications

Processing of Lipoaspirate Samples for Optimal Mesenchymal Stem Cells Isolation

Processing of Lipoaspirate Samples for Optimal Mesenchymal Stem Cells Isolation

A small population of resident limb bud mesenchymal cells express few MSC‐associated markers, but the expression of these markers is increased immediately after cell culture

Isolation of Ready‐to‐Use Adipose‐Derived Stem Cell (ASC) Pellet for Clinical Applications and a Comparative Overview of Alternate Methods for ASC Isolation

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