An alternative method for medium-term in vitro conservation of different plant species through gibberellin inhibitors

Spinoso-Castillo, José Luis; Pérez-Sato, Juan Antonio; Schettino-Salomón, Sandra Silvana; Bello-Bello, Jericó Jabín

doi:10.1007/s11627-022-10263-y

Cited by 3 publications

(8 citation statements)

References 27 publications

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“…Regarding root length, the smallest roots were observed in the treatments with 1 and 2 mg L −1 of PAC. Spinoso-Castillo et al [7] found an increased number of shoots in sugarcane during in vitro conservation at a concentration of 1 mg L −1 of PAC. Zeid et al [11] observed an increased number of microtubers per explant in S. tuberosum during in vitro conservation at a concentration of 3 mg L −1 of PAC.…”

Section: Effect Of Pac and Agnps On Physiological And Biochemical Par...mentioning

confidence: 98%

“…), anthurium (Anthurium andraeanum) and agave (Agave potatorum) [7]. Spinoso-Castillo et al [7] found an increase in stomatal density during the in vitro conservation of Saccharum spp., A. andraeanum and A. potatorum at a concentration of 3 mg L −1 PAC. The cell size could be due to an inhibition of cell elongation caused by PAC, and probably due to decreased ethylene effects in tissues by AgNPs, or due to the combined effect of PAC × AgNPs.…”

Section: Effect Of Pac and Agnps On Physiological And Biochemical Par...mentioning

confidence: 99%

“…The use of GA inhibitors in in vitro conservation has been reported in sugarcane (Saccharum spp.) [7,20], potato (S. tuberosum) [11], native orchid (Stanhopea tigrina Bateman ex Lind) [21] and anthurium (Anthurium andraeanum L.) [7].…”

Section: Effect Of Pac and Agnps On Physiological And Biochemical Par...mentioning

confidence: 99%

“…Variation in stomatal density using PAC has been reported in different plant species such as sugarcane (Saccharum spp. ), anthurium (Anthurium andraeanum) and agave (Agave potatorum) [7]. Spinoso-Castillo et al [7] found an increase in stomatal density during the in vitro conservation of Saccharum spp., A. andraeanum and A. potatorum at a concentration of 3 mg L −1 PAC.…”

Section: Effect Of Pac and Agnps On Physiological And Biochemical Par...mentioning

confidence: 99%

“…Medium-term in vitro conservation has more advantages than short-term periods, such as control over storage conditions, and compared to long-term periods that require a constant supply of liquid nitrogen for cryopreservation, which is not cost effective for use in commercial laboratories [5,6]. In addition, medium-term in vitro conservation allows for slow-growth storage, reduces labor costs and allows for the availability of the germplasm bank [7].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Conservation and Regeneration of Potato (Solanum tuberosum L.): Role of Paclobutrazol and Silver Nanoparticles

Bernal¹,

Spinoso-Castillo²,

Mancilla-Álvarez³

et al. 2023

Horticulturae

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In vitro conservation and regeneration of potato germplasm is important in breeding programs. The objective of this study was to assess the combined effect of paclobutrazol (PAC) and silver nanoparticles (AgNPs) during reduced-growth conservation and their in vitro ability to regenerate S. tuberosum after conservation treatments. For the conservation system, apices were used as explants in Murashige and Skoog culture media with different combinations of PAC (0, 0.5, 1 and 2 mg L−1) and AgNPs (0, 50, 100 and 200 mg L−1). At six months of culture, plant length, number of lateral branches, number of leaves and roots, root length, stomatal density and total chlorophyll content were assessed. For regeneration, explants were internodes cultured in medium with only 2 mg L−1 benzyladenine (BA). At 60 d of culture, the response percentage, number of shoots per explant and shoot length were assessed. For in vitro conservation, the combination of 2 mg L−1 PAC and 50 mg L−1 AgNPs was the best treatment, whereas for in vitro regeneration, the highest number of shoots was in explants that were treated with 1 or 2 mg L−1 PAC with 50 mg L−1 AgNPs. In conclusion, PAC and AgNPs are alternatives for in vitro S. tuberosum conservation, and their evaluation in other species recalcitrant to the effect of ethylene is recommended.

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Section: Effect Of Pac and Agnps On Physiological And Biochemical Par...mentioning

confidence: 98%

Section: Effect Of Pac and Agnps On Physiological And Biochemical Par...mentioning

confidence: 99%

Section: Effect Of Pac and Agnps On Physiological And Biochemical Par...mentioning

confidence: 99%

Section: Effect Of Pac and Agnps On Physiological And Biochemical Par...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In Vitro Conservation and Regeneration of Potato (Solanum tuberosum L.): Role of Paclobutrazol and Silver Nanoparticles

Bernal¹,

Spinoso-Castillo²,

Mancilla-Álvarez³

et al. 2023

Horticulturae

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In vitro germination and development of “Canelita” (Lycaste aromatica (Graham) Lindl.) in gravity immersion bioreactors

Solís-Zanotelli

Baltazar-Bernal

Cruz-Huerta

et al. 2022

In Vitro Cell.Dev.Biol.-Plant

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The orchid Lycaste aromatica (Graham) Lindl. is known for its outstanding cinnamon aroma, which has caused its illegal extraction from its natural habitat. For this reason, procedures were developed for the asymbiotic seeds germination and seedlings development in a gravity immersion bioreactor (GIB) system. Four culture media were tested for in vitro germination: ½ Murashige and Skoog (MS), MS, ½ Knudson C 15 g L − 1 sucrose, Knudson C. The e ciency of the in vitro culture protocol using the semi-solid medium was compared and with four immersion frequencies of the GIB system in a liquid medium, using 30 mL of ½ MS medium per seedling. Germination began at eight weeks and lasted for six weeks. The best treatment was ½ MS with 161 germinated seeds. In 90 days, the cultures in bioreactors reached signi cant differences in the number of leaves (10 leaves), number of roots (4.7 roots), and seedling height (5.4 cm). However, the seedlings developed in the semi-solid medium reported a higher number of shoots (1.6 shoots) and root length (2 cm); furthermore, the bioreactor culture showed the highest photosynthesis rate (0.74 µmol CO 2 g − 1 s − 1 ). The culture system in bioreactors is an excellent alternative to develop this species because it increases photosynthesis and the size of the seedlings, which favor their acclimatization. Key MessageLycaste aromatica begins germination in vitro at 8 weeks, lasts for six weeks, develops functional stomata, and promotes photosynthesis in the gravity immersion bioreactor.

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In vitro Tuberization using Silicon Nanoparticles and short-term cold Storage of mini-tubers of Dioscorea pentaphylla L.

M.,

Dey,

Faisal

et al. 2023

BioNanoSci.

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An alternative method for medium-term in vitro conservation of different plant species through gibberellin inhibitors

Cited by 3 publications

References 27 publications

In Vitro Conservation and Regeneration of Potato (Solanum tuberosum L.): Role of Paclobutrazol and Silver Nanoparticles

In Vitro Conservation and Regeneration of Potato (Solanum tuberosum L.): Role of Paclobutrazol and Silver Nanoparticles

In vitro germination and development of “Canelita” (Lycaste aromatica (Graham) Lindl.) in gravity immersion bioreactors

In vitro Tuberization using Silicon Nanoparticles and short-term cold Storage of mini-tubers of Dioscorea pentaphylla L.

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