An alternative in vitro plant regeneration system in papaya ( Carica papaya L.) through callus derived nodular cultures

Anandan, R.; Deenathayalan, T.; Kumar, Nukala Sumanth; Deepak, K.V.

doi:10.1016/j.mgene.2018.06.007

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…For the induction of callus cultures, we preferred ms medium containing lower concentrations of cytokinin and higher concentration of auxin to be the best combination. Our experiments showed NAA with BAP to be essential for callus induction and growth, in agreement with previous reports on Solanum dubium [24] Carica papaya [25].…”

Section: Discussionsupporting

confidence: 93%

In-vitro Regeneration from Direct and Indirect Organogenesis of Crescentia alata Kunth an Important Multipurpose Medicinal Tree

Abinaya¹

2021

EJMP

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In this present work, an in-vitro regeneration protocol for Crescentia alata (C. alata) was developed using various explants on Murashige and Skoog (MS) medium augmented with different concentrations and combinations of plant growth regulators (PGRs) for direct and indirect regeneration. The direct organogenesis was established from nodes and internodes on MS medium supplemented with cytokinins and auxins. The indirect organogenesis via callus phase was obtained from leaf, nodes and internodes on MS medium supplemented with different concentrations of PGRs. The high frequency shoot organogenesis were achieved directly from nodal explants were cultured on MS medium supplemented with 3.0 mg/L BAP+0.5 mg/L KIN +1.0 mg/L NAA. Indirect organogenesis callogenic frequency was optimized at the concentration of MS medium containing 1.0 mg/L BAP + 5.0 mg/L IAA. The callus was obtained from all the explants were used, among these explants internodal explants gave best result on MS medium supplemented with different concentrations of cytokinins and auxins for indirect organogenesis experiment. Indirect organogenesis the highest number of shoot regeneration was obtained in MS Basal Medium with 4.0 mg/L BAP + 0.5 mg/L KIN + 2.0 mg/L NAA from internodal explants. For root formation the regenerative shoots which were sub cultured on MS medium containing different ratios of auxins. The rooted plantlets were transferred successfully to the pots containing sterilized soil and were successfully hardened at greenhouse condition for 20 days then exposed to the natural environment. This is the first successful micropropagation report of an efficient and rapid in-vitro clonal propagation protocol for C. alata by direct and indirect shoot organogenesis through various explants, which can be employed for conservation of this important medicinal tree species as well as the utilization of an biologically important active biomolecules. This protocol can be very useful to obtain plants from various explants, without the requirement of meristematic regions, enabling the obtainment of a higher number of plants in short period.

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Section: Discussionsupporting

confidence: 93%

In-vitro Regeneration from Direct and Indirect Organogenesis of Crescentia alata Kunth an Important Multipurpose Medicinal Tree

Abinaya¹

2021

EJMP

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“…1f) were observed under stereomicroscope after 60-70 days of culturing of embryogenic calli on embryo induction medium with 2,4 For the effective maturation of somatic embryos to the cotyledonary stage, embryogenic cultures were transferred to MS medium fortified with different concentrations of ABA or in combination with different concentrations of BAP ( Among the treatments, MS media fortified with ABA (1.5 mg/L) in combination with BAP (0.4 mg/L) recorded significantly highest per cent cotyledonary embryos (52.50%) ( Table 2). Similar results were obtained by including the maturation medium which increased embryo size and improved somatic embryo development [18,19,20,7,5,1]. This is in contrast with the report given by Litz and Conver et al [3], wherein the somatic embryos of papaya matured in the absence of exogenous growth regulators.…”

Section: Maturation Of Somatic Embryossupporting

confidence: 75%

Somatic Embryogenesis in Papaya (Carica papaya L. cv. TNAU Papaya CO.8)

Rajesh

Sudhakar

Kumar

et al. 2020

CJAST

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An efficient indirect somatic embryogenesis protocol for Carica papaya var TNAU Papaya CO.8 was developed using immature zygotic embryos as an explant. Two growth regulators namely 2,4-D and picloram each at 1, 2, 3 mg/L were tested for callus induction and the highest callus induction frequency (83.33%) was observed in MS medium supplemented with 3 mg/L 2,4-D. However the rate of conversion into somatic embryos was highest (63.33%) on MS medium supplemented with 2 mg/L 2,4-D. Maturation of somatic embryos was studied by using MS medium with different concentrations of abscisic acid (ABA) and benzyl amino purine (BAP) along with glutamine (400 mg/L). The maturation of globular embryos was observed to be higher in the combination of ABA (1.5 mg/L), BAP (0.4 mg/L) along with glutamine (400 mg/L). Even though regeneration was observed from cotyledonary stage embryos in presence of different growth regulators like BAP, α-naphthalene acetic acid (NAA), phloridzin dehydrate kinetin and gibberellic acid, further growth was not observed due to abnormal regenerative structures. Regeneration of cotyledonary stage somatic embryos were highest (77.4%) in half strength MS medium without growth regulators. The well-developed plantlets with shoots and roots were subsequently transferred for hardening.

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“…Micropropagation by tissue culture offers advantages over conventional propagation methods and has been widely applied to papaya species. Different approaches have been followed, such as axillary shoot culture [5][6][7][8] and plantlet recovery after shoot regeneration Horticulturae 2023, 9, 671. https://doi.org/10.3390/horticulturae9060671 https://www.mdpi.com/journal/horticulturae from somatic embryos [9][10][11][12][13][14][15][16]. This regeneration and plant recovery often occurs after genetic transformation procedures in this species [11,[17][18][19][20][21][22][23][24], and it highlights the importance of developing reliable methods of preservation for long periods of time in order to finally obtain high-quality plants from selected lines of genetically modified papaya.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Long-Term Cultures of Papaya (Carica papaya L. cv. Solo)

2023

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In this article, we present proliferation data from 10 years of the continuous in vitro incubation of cv. Solo papaya shoots and propose a reliable method for the long-term micropropagation of papaya, using microshoots developed from the axillary buds of papaya shoots as primary explants. Three different media were assayed. The proliferation medium (PPRM) allowed us to maintain papaya shoots under continuous proliferation for 20 years, maintaining consistent behavior. Most of the shoots developed in the PPRM rooted during the incubation and then acclimated easily, maintaining the ploidy and morphological characteristics of the parental plants, and flowering and setting fruits normally. The PPRM medium consisted of MS medium supplemented with naphthalene acetic acid (NAA) (0.1 mg L−1), benzyladenine (BA) (0.5 mg L−1), gibberellic acid (GA3) (0.5 mg L−1), and adenine hemisulphate (40 mg L−1). The average multiplication rate was higher than 20 shoots per explant during the long-term assay. The elongation medium (PELM) was designed to recover shoots with poor growth and allowed the development of high-quality shoots ready for rooting. It consisted of an MS basal medium supplemented with NAA (0.1 mg L−1), kinetin (KIN) (0.5 mg L−1), and GA3 (1 mg L−1). The rooting medium (PROM) was designed to induce high-quality roots from nonrooted shoots and consisted of a half-strength MS medium and indole-3-butiryc acid (IBA) (1 mg L−1). On PROM, agar can be exchanged for expanded vermiculite. Acclimation took place inside an acclimatization tunnel under progressive hydric stress. After 4 weeks, the plant recovery rate was 90% for plants maintained under continuous proliferation for ten years. The main objective of this work was to provide a micropropagation method which would maintain healthy elite genotypes of papaya for long periods of time and produce a high number of good quality plants.

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An alternative in vitro plant regeneration system in papaya ( Carica papaya L.) through callus derived nodular cultures

Cited by 5 publications

References 36 publications

In-vitro Regeneration from Direct and Indirect Organogenesis of Crescentia alata Kunth an Important Multipurpose Medicinal Tree

In-vitro Regeneration from Direct and Indirect Organogenesis of Crescentia alata Kunth an Important Multipurpose Medicinal Tree

Somatic Embryogenesis in Papaya (Carica papaya L. cv. TNAU Papaya CO.8)

In Vitro Long-Term Cultures of Papaya (Carica papaya L. cv. Solo)

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