An alternative, banana peel-based medium used to investigate the catalytic properties of peroxidase from a fungus, Inonotus sp SP2, recently isolated in southern Chile

Garcés, Lucía Atehortúa; Vásquez, Carlos H.; Contreras, Elsa Galarza; Urra, Johana; Díez, M.C.; Guerrero, Lorna; Palma, C.

doi:10.1007/s12257-012-0295-8

Cited by 7 publications

(4 citation statements)

References 32 publications

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“…Lower reaction rate values were attained by the EF generated by SSF, but reaction rate was satisfactorily impacted to a lesser extent, presenting a diminished slope. This might suggest the presence of organic acids in banana peel that reduce inhibitory effects of H 2 O 2 , [34] further supporting the proposal of this residue as an alternative to conventional culture medium.…”

Section: Enzymatic Kinetic Studysupporting

confidence: 70%

“…(2); the corresponding results besides the correlation coefficients obtained for various H 2 O 2 concentrations are shown in Table 2: These experiments were performed by the EFs collected from the SF and SSF assays, although it could be also interesting to obtain and evaluate the crude enzyme. However, Garcés et al [34] proved that the maximum reaction rate reached with the crude enzyme is markedly lower (about 50%) than that found with the extracellular medium from the fermentation under solid-state conditions of Inonotus sp. SP2 using banana peel.…”

Section: Enzymatic Kinetic Studymentioning

confidence: 93%

“…[32,33] In addition, previous works reported the successful use of banana peel as energy and nutrients source for the production of MnP from Inonotus sp. SP2 by SF [34] ; nevertheless, the catalytic properties of VP produced from P. eryngii by this unconventional growth medium remain uncharacterized.…”

Section: Introductionmentioning

confidence: 99%

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Production of versatile peroxidase fromPleurotus eryngiiby solid-state fermentation using agricultural residues and evaluation of its catalytic properties

Palma

Lloret

Sepúlveda

et al. 2015

Preparative Biochemistry & Biotechnology

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Interest in production of ligninolytic enzymes has been growing over recent years for their use in various applications such as recalcitrant pollutants bioremediation; specifically, versatile peroxidase (VP) presents a great potential due to its catalytic versatility. The proper selection of the fermentation mode and the culture medium should be an imperative to ensure a successful production by an economic and available medium that favors the process viability. VP was produced by solid-state fermentation (SSF) of Pleurotus eryngii, using the agricultural residue banana peel as growth medium; an enzymatic activity of 10,800 U L(-1) (36 U g(-1) of substrate) was detected after 18 days, whereas only 1800 U L(-1) was reached by conventional submerged fermentation (SF) with glucose-based medium. The kinetic parameters were determined by evaluating the H2O2 and Mn(2+) concentration effects on the Mn(3+)-tartrate complex formation. The results indicated that although the H2O2 inhibitory effect was observed for the enzyme produced by both media, the reaction rates for VP obtained by SSF were less impacted. This outcome suggests the presence of substances released from banana peel during the fermentation, which might exhibit a protective effect resulting in an improved kinetic behavior of the enzyme.

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Section: Enzymatic Kinetic Studysupporting

confidence: 70%

Section: Enzymatic Kinetic Studymentioning

confidence: 93%

See 1 more Smart Citation

Production of versatile peroxidase fromPleurotus eryngiiby solid-state fermentation using agricultural residues and evaluation of its catalytic properties

Palma

Lloret

Sepúlveda

et al. 2015

Preparative Biochemistry & Biotechnology

Self Cite

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“…In addition, a series of physiological and biochemical changes occurred during the storage period of bananas. For example, soluble sugar (Yang et al, 2009), peroxidase (POD; Garcés et al, 2012), and polyphenol oxidase (PPO; Lee, 2007) in banana fruit progressively increased and polyamines (Lima et al, 2003) decreased with the prolonging of storage time. It is considered that ethylene binds to ethylene receptors, thereby eliciting signal transduction process which leads to normal ripening of bananas (Fluhr and Mattoo, 1996).…”

Section: Introductionmentioning

confidence: 99%

Effect of 1-pentylcyclopropene on Physiological Responses and Gene Expression of Ethylene Receptors in Post-Harvest Bananas

Feng

Wang

2014

Food Biotechnology

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The aim of this study was to investigate the inhibitory effect of 1-pentylcyclopropene (1-PentCP), a structural analogue of 1-methylcyclopropene (1-MCP), on postharvest ripening and senescence of bananas fruit (Musa acuminata L. AAA group cv. Brazil). The results showed that 1-PentCP was effective in postponing the occurrence time of ethylene production peak and respiration rate peak, delaying the softening and color change, inhibiting the increasing of soluble solids, soluble sugar, and 1-aminocyclopropane-1-carboxylate acid (ACC) content; inhibiting the activities of ACC synthase (ACS) and ACC oxidase (ACO); and suppressing the expression of MaERS1 and MaERF1 in bananas. In addition, 1.2 µL/L 1-PentCP was found to be most effective in delaying ripening and senescence process of bananas by inhibiting the activities of enzymes involved in ethylene biosynthesis and gene expression of ethylene receptors. It is suggested that 1-PentCP could be a promising candidates for practical use as a given ethylene inhibitor applied before the onset of ethylene action.

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Method for the stabilization and immobilization of enzymatic extracts and its application to the decolorization of textile dyes

et al. 2014

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Peroxidases from Pleurotus eryngii have been investigated for their ability to degrade recalcitrant, phenolic pollutants. The use of crude enzymatic extracts can reduce the high costs associated with enzyme purification, and enzyme immobilization can enhance enzyme stability and recovery. The present study tests the effectiveness of various conditions for crude enzyme stabilization in polyethylene glycol and glycine solutions, and immobilization on monofunctional and heterofunctional agarose solid supports. Glycine at 0.5 M at 4 °C and pH 4 was most effective stabilization agent for the crude enzymatic extracts, and enzyme immobilization efficiency was greatest for heterofunctional supports. MANA-glyoxyl heterofunctional supports were demonstrated to have the greatest enhancement of decolorization (1.3-fold) and velocity of substrate consumption (fivefold). Therefore, the application of crude enzymatic extracts to industrial processes, such as dye decolorization, represents a cost-effective alternative to purified enzymes.

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An alternative, banana peel-based medium used to investigate the catalytic properties of peroxidase from a fungus, Inonotus sp SP2, recently isolated in southern Chile

Cited by 7 publications

References 32 publications

Production of versatile peroxidase fromPleurotus eryngiiby solid-state fermentation using agricultural residues and evaluation of its catalytic properties

Production of versatile peroxidase fromPleurotus eryngiiby solid-state fermentation using agricultural residues and evaluation of its catalytic properties

Effect of 1-pentylcyclopropene on Physiological Responses and Gene Expression of Ethylene Receptors in Post-Harvest Bananas

Method for the stabilization and immobilization of enzymatic extracts and its application to the decolorization of textile dyes

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