An Alteration of Lymphocytes Subpopulations and Immunoglobulins Levels in Patients with Diabetic Foot Ulcers Infected Particularly by Resistant Pathogens

Fejfarová, Vladimı́ra; Jirkovská, Alexandra; Dubský, Michal; Game, Frances; Vydláková, Jana; Sekerkova, Alena; Franeková, Jana; Kučerová, Monika; Střı́ž, Ilja; Petkov, V.; Bém, Robert; Wosková, Veronika; Němcová, Andrea; Skibová, J

doi:10.1155/2016/2356870

Cited by 16 publications

(17 citation statements)

References 38 publications

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“…The activation of adaptive immunity is mediated by the production of antibodies by B lymphocytes, which in turn neutralize the pathogens in circulation and subsequently activate complement system . Thus, abnormal activation of adaptive immunity exemplified by the production of auto‐antibodies against nuclear proteins is a hallmark of IDFU and implicit in this association is B cell dysfunction …”

Section: Introductionmentioning

confidence: 99%

Circulatory levels of B‐cell activating factor of the TNF family in patients with diabetic foot ulcer: Association with disease progression

Umapathy

Rajan

Kumar

et al. 2019

Wound Repair Regeneration

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Enhanced and prolonged expression of tumor necrosis factor alpha (TNF-α), a potent pro-inflammatory cytokine is evidenced during the chronic wound healing process of infected diabetic foot ulcer (IDFU). B-cell activating factor (BAFF) is the member of TNF-α family, which implicit in B-cell dysfunction. This study was aimed to evaluate the role of BAFF in diabetic foot ulcer (DFU) patients and to correlate its association with other family of inflammatory cytokines. Circulating levels of BAFF and other cytokines were measured in IDFU (n = 44) and non-IDFU patients (n = 40) using multiplexed bead-based cytokine immunoassay. A stepwise significant increase was observed in both circulatory BAFF and C-reactive protein (CRP) during the disease progression. The area under the receiver operating characteristic curve (AUC ROC ) for BAFF was found to be high (0.89; [95% CI: 0.73-1.0]), when compared to CRP (0.68; [95% CI: 0.61-0.76]). Optimum diagnostic cutoff level for BAFF was found to be ≥2.35 pg/mL with 62.0% sensitivity and 85.7% specificity. Further, BAFF levels showed a significant positive correlation with CRP among IDFU patients. With respect to other family cytokines, BAFF levels were positively correlated with TNF-α, interferon family cytokines such as IFN-α2, IL-28A/IFN-λ2, IFN-γ, and IL-10 family cytokines such as IL-19, IL-22, and IL-26 and negatively correlated with IL-6 receptor family such as gp130/sIL-6Rβ. Hence, our data suggest that devising therapeutic strategies to reduce the levels of BAFF may contribute in amelioration of IDFU.

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Section: Introductionmentioning

confidence: 99%

Circulatory levels of B‐cell activating factor of the TNF family in patients with diabetic foot ulcer: Association with disease progression

Umapathy

Rajan

Kumar

et al. 2019

Wound Repair Regeneration

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“…Several immunological defects have been reported in patients with DM, such as altered phagocytosis and bactericidal activity of polymorphonuclear cells; impaired chemotaxis and phagocytosis functions of monocyte/macrophage; disturbances of cellular innate immunity, including a low serum level of complement factor 4 (C4) and abnormal production of cytokines by monocytes [17]; and alteration of lymphocytes subpopulations and immunoglobulins levels [18]. These abnormalities, mostly related to innate immunity, seem to play a role in the susceptibility of diabetic patients to infections, particularly by resistant pathogens [19].…”

Section: Etiology Of Diabetic Foot Ulcersmentioning

confidence: 99%

Current Therapeutic Strategies in Diabetic Foot Ulcers

et al. 2019

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Diabetic foot ulcers (DFUs) are the fastest growing chronic complication of diabetes mellitus, with more than 400 million people diagnosed globally, and the condition is responsible for lower extremity amputation in 85% of people affected, leading to high-cost hospital care and increased mortality risk. Neuropathy and peripheral arterial disease trigger deformities or trauma, and aggravating factors such as infection and edema are the etiological factors for the development of DFUs. DFUs require identifying the etiology and assessing the co-morbidities to provide the correct therapeutic approach, essential to reducing lower-extremity amputation risk. This review focuses on the current treatment strategies for DFUs with a special emphasis on tissue engineering techniques and regenerative medicine that collectively target all components of chronic wound pathology.

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“…While the role of CD8 + and CD4 + lymphocytes in wound healing in the presence of either tissue ischemia or diabetes has not been investigated in animals, few data are available in humans. Diabetic individuals with foot ulceration showed a decreased number of total circulating lymphocytes [ 129 ], with a relative accumulation of activated T cells and increased TNFα levels, indicative of a proinflammatory condition [ 130 ]. Overall, this evidence suggests that altered balance of T cell sub-populations may impact on the closure of diabetic wounds, yet further studies are needed to characterize their precise role.…”

Section: Lymphocytesmentioning

confidence: 99%

Immune Cell Therapies to Improve Regeneration and Revascularization of Non-Healing Wounds

Groppa

Colliva

Vuerich

et al. 2020

IJMS

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With the increased prevalence of chronic diseases, non-healing wounds place a significant burden on the health system and the quality of life of affected patients. Non-healing wounds are full-thickness skin lesions that persist for months or years. While several factors contribute to their pathogenesis, all non-healing wounds consistently demonstrate inadequate vascularization, resulting in the poor supply of oxygen, nutrients, and growth factors at the level of the lesion. Most existing therapies rely on the use of dermal substitutes, which help the re-epithelialization of the lesion by mimicking a pro-regenerative extracellular matrix. However, in most patients, this approach is not efficient, as non-healing wounds principally affect individuals afflicted with vascular disorders, such as peripheral artery disease and/or diabetes. Over the last 25 years, innovative therapies have been proposed with the aim of fostering the regenerative potential of multiple immune cell types. This can be achieved by promoting cell mobilization into the circulation, their recruitment to the wound site, modulation of their local activity, or their direct injection into the wound. In this review, we summarize preclinical and clinical studies that have explored the potential of various populations of immune cells to promote skin regeneration in non-healing wounds and critically discuss the current limitations that prevent the adoption of these therapies in the clinics.

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An Alteration of Lymphocytes Subpopulations and Immunoglobulins Levels in Patients with Diabetic Foot Ulcers Infected Particularly by Resistant Pathogens

Cited by 16 publications

References 38 publications

Circulatory levels of B‐cell activating factor of the TNF family in patients with diabetic foot ulcer: Association with disease progression

Circulatory levels of B‐cell activating factor of the TNF family in patients with diabetic foot ulcer: Association with disease progression

Current Therapeutic Strategies in Diabetic Foot Ulcers

Immune Cell Therapies to Improve Regeneration and Revascularization of Non-Healing Wounds

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