An alpha 1/beta 1-like integrin receptor on rat aortic smooth muscle cells mediates adhesion to laminin and collagen types I and IV.

Clyman, Ronald I.; Turner, David C.; Kramer, Randall H.

doi:10.1161/01.atv.10.3.402

Cited by 51 publications

(28 citation statements)

References 35 publications

(15 reference statements)

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“…This double specificity has been reported also in other cellular systems (Clyman et aL, 1990). It is of interest that neurite elongation by RA-treated cells expressing high levels of al/01 is more pronounced on laminin than on collagen type 1.…”

Section: Discussionsupporting

confidence: 72%

Up-regulation of the integrin alpha 1/beta 1 in human neuroblastoma cells differentiated by retinoic acid: correlation with increased neurite outgrowth response to laminin.

et al. 1991

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Retinoic acid (RA) is known to induce differentiation of neuroblastoma cells in vitro. Here we show that treatment of two human neuroblastoma cell lines, SY5Y and IMR32, with RA resulted in a fivefold increase of the integrin alpha 1/beta 1 expression. The effect was selective because expression of the alpha 3/beta 1 integrin, also present in these cells, was not increased. The up-regulation of the alpha 1/beta 1 differentiated SY5Y cells correlated with increased neurite response to laminin. In fact, RA-treated SY5Y cells elongated neurites on laminin-coated substratum more efficiently compared with untreated cells or cells treated with nerve growth factor, insulin, or phorbol 12-myristate 13-acetate. These three agents induced partial morphological differentiation but did not increase alpha 1 integrin expression. Neurite extension in RA-treated cells was more efficient on laminin than on fibronectin or collagen type I and was inhibited with beta 1 integrin antibodies on all three substrates. Affinity chromatography experiments showed that alpha 1/beta 1 is the major laminin receptor in both untreated and RA-treated SY5Y cells. These data show that RA, a naturally occurring morphogen implicated in embryonic development, can selectively regulate the expression of integrin complexes in neuronal cells and suggest an important role of the alpha 1/beta 1 laminin receptor in the morphological differentiation of nerve cells.

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Section: Discussionsupporting

confidence: 72%

Up-regulation of the integrin alpha 1/beta 1 in human neuroblastoma cells differentiated by retinoic acid: correlation with increased neurite outgrowth response to laminin.

et al. 1991

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“…The characterization of the antibodies used in this investigation was referenced in earlier publications (1,18,19). Neither the mouse MAb against human av (LM 142) nor the rat MAb against human as (B]E s) bound to the corresponding ovine a subunits; theywere used as control antibodies when other mouse and rat antibodies were tested.…”

Section: Antibodiesmentioning

confidence: 99%

“…Both LN and type IV collagen were isolated from Englebreth Holm Swarm tumors grown in C57 BL/6 mice (22). The LN used in these experiments was found to be free of type IV collagen and entactin (18).…”

Section: Ecmmentioning

confidence: 99%

“…Adhesion of DA-SMC to protein-coated microtiter plates was assayed as described previously (18). Confluent cell monolayers were washed with medium without FCS and were incubated with or without TGF~l (250 pM) for an additional 22 h. Cells were removed from the tissue culture plates by brief incubation (2 min) at room temperature with trypsin -EDTA.…”

Section: Adhesionmentioning

confidence: 99%

“…The cells were suspended in 1 mL of iodination buffer, containing 200 mU/mL glucose oxidase and 200 JLglmL (20 U/mL) lactoperoxidase, and were surfacelabeled with! mCi/inLNa l 25I as previously described (18). An aliquot of the cell suspension was used to determine cell number.…”

Section: Cell Surface Radioiodinationmentioning

confidence: 99%

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Transforming Growth Factor β1 Inhibits Fetal Lamb Ductus Arteriosus Smooth Muscle Cell Migration

et al. 1995

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Anatomical closure of the ductus arteriosus (DA) requires normally quiescent smooth muscle cells (SMC) to migrate out of the muscle media into the subendothelial space, forming intimal mounds that eventually coalesce to occlude the vessel's lumen. Transforming growth factor-B, (TGF{31)' a potent modulator of vascular SMC migration, is found in the wall of the closing DA. We examined the effect of TGF{31 on the migration of fetal lamb DA-SMC. Although TGF{31 has been shown to be a chemoattractant for other mesenchymal cells, it had no chemotactic effect on DA-SMC; furthermore, TGF{31 did not enhance the migration of DA-SMC (as has been reported for aortic SMC). Rather, incubating DA-SMC with TGF{31 for 22 h decreased the rate of migration of SMC on extracellular matrix substrata composed of fibronectin, vitronectin, laminin, and collagen I and IV. Exposure of DA-SMC to TGF{31 was associated with an increase in the formation of focal adhesion plaques (tight associations between the cells' surface and extracellular matrix). DA-SMC use integrin receptors to attach to and migrate on extracellular matrix components. The decrease in DA-SMC migration was not associated with a significant change in the profile of integrin receptors Closure of the DA is a necessary adaptation to extrauterine life . Term infants functionally close their DA within the first several hours of life , followed by vessel w all reorganization and permanent anatomical clo sur e. Preterm infants, however, frequently show initial constriction of the muscular media (functional closure), but fail to initiate vessel wall remodeling, leading to patency of the DA and its associated morbidity.DA remodeling is dependent upon the migration of SMC from the muscle media into the subendothelial space; this forms the intimal mounds nec essary for obliteration of the vessel 's lumen and permanent anatomical closure. For migra-

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Measurement of individual cell migration parameters for human tissue cells

et al. 1992

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An alpha 1/beta 1-like integrin receptor on rat aortic smooth muscle cells mediates adhesion to laminin and collagen types I and IV.

Cited by 51 publications

References 35 publications

Up-regulation of the integrin alpha 1/beta 1 in human neuroblastoma cells differentiated by retinoic acid: correlation with increased neurite outgrowth response to laminin.

Up-regulation of the integrin alpha 1/beta 1 in human neuroblastoma cells differentiated by retinoic acid: correlation with increased neurite outgrowth response to laminin.

Transforming Growth Factor β1 Inhibits Fetal Lamb Ductus Arteriosus Smooth Muscle Cell Migration

Measurement of individual cell migration parameters for human tissue cells

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