An alkaline thermostable laccase from termite gut associated strain of Bacillus stratosphericus

Xiao, Yuanxi; Li, Jing; Wu, Ping; Na, Ning; Li, Jingjing; Shen, Yulong; Huang, Qihong; Ni, Jinfeng

doi:10.1016/j.ijbiomac.2021.02.205

Cited by 15 publications

(8 citation statements)

References 70 publications

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“…Ghlac lost more than 60% of its original activity after incubation at pH 3.0 for 6 h, while Mut2 retained more than 95% of the original activity over the pH range of 4.0 to 8.0 (Figure 3C), similar to the characterized DUF152 laccases [22][23][24]. The bacterial laccases from B. stratosphericus, γ-proteobacterium, and a marine microbial metagenomic library showed high tolerance for alkaline conditions, which is an advantageous property of laccases from bacteria [27][28][29]. By contrast, most of the laccases from fungi are unstable under alkaline conditions [5,30].…”

Section: Effects Of Ph and Temperature On The Activity And Stability Of Ghlacmentioning

confidence: 56%

“…Although the activity of Mut2 was not strongly affected by 1 mM Zn 2+ and Ba 2+ , it was dramatically reduced to 47% and 0, respectively, when the metal ions concentration increased to 10 mM. This inhibitory effects of high-concentration Zn 2+ or Ba 2+ were generally reported in laccases such as BaCotA, PvL, SN4LAC, and MSKLAC [27,[36][37][38]. As the essential factor for the activity of Mut2, 10 mM Cu 2+ did not show any inhibitory effect on Mut2 (Figure 4C).…”

Section: Effects Of Metal Ions and Organic Solvents On The Activity Of Mut2mentioning

confidence: 74%

“…This kind of boosted laccase activity was also reported in laccases from Cerrena sp. RSD1, T. versicolor, and a marine metagenomic library [39,40], whereas laccases from B. stratosphericus, Ganoderma lucidum, and Sporothrix carnis were strongly inhibited by 10% dimethyl sulfoxide [27,41,42]. Moreover, Mut2 could retain 90% of its activity in the presence of 10% formaldehyde, which is a powerful protein denaturant, and could strongly inhibit the laccase from B. amyloliquefaciens [43].…”

Section: Effects Of Metal Ions and Organic Solvents On The Activity Of Mut2mentioning

confidence: 99%

“…However, conventional physical and chemical treatment processes usually produce secondary sludge and hazardous byproducts, resulting in serious environmental pollution [44,45]. Laccase, as a green catalyst, has drawn increasing attention, being a potential alternative to tackle dye pollution in an environmentally friendly manner [27,45,46].…”

Section: Mg Decolorization Catalyzed By Mut2mentioning

confidence: 99%

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An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green

Mao

Wang

et al. 2021

IJMS

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Laccases can catalyze the remediation of hazardous synthetic dyes in an eco-friendly manner, and thermostable laccases are advantageous to treat high-temperature dyeing wastewater. A novel laccase from Geothermobacter hydrogeniphilus (Ghlac) was cloned and expressed in Escherichia coli. Ghlac containing 263 residues was characterized as a functional laccase of the DUF152 family. By structural and biochemical analyses, the conserved residues H78, C119, and H136 were identified to bind with one copper atom to fulfill the laccase activity. In order to make it more suitable for industrial use, Ghlac variant Mut2 with enhanced thermostability was designed. The half-lives of Mut2 at 50 °C and 60 °C were 80.6 h and 9.8 h, respectively. Mut2 was stable at pH values ranging from 4.0 to 8.0 and showed a high tolerance for organic solvents such as ethanol, acetone, and dimethyl sulfoxide. In addition, Mut2 decolorized approximately 100% of 100 mg/L of malachite green dye in 3 h at 70 °C. Furthermore, Mut2 eliminated the toxicity of malachite green to bacteria and Zea mays. In summary, the thermostable laccase Ghlac Mut2 could effectively decolorize and detoxify malachite green at high temperatures, showing great potential to remediate the dyeing wastewater.

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Section: Effects Of Ph and Temperature On The Activity And Stability Of Ghlacmentioning

confidence: 56%

Section: Effects Of Metal Ions and Organic Solvents On The Activity Of Mut2mentioning

confidence: 74%

Section: Effects Of Metal Ions and Organic Solvents On The Activity Of Mut2mentioning

confidence: 99%

Section: Mg Decolorization Catalyzed By Mut2mentioning

confidence: 99%

See 2 more Smart Citations

An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green

Mao

Wang

et al. 2021

IJMS

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“…The aforementioned potent strain for oxidative enzyme production was subjected to the 16S rRNA gene analysis for its identification. Recently, Xiao et al [ 66 ] has investigated the multiple enzymatic properties of laccase derived from hindgut microbes of fungus growing termites ( Macrotermes barneyi. ).…”

Section: Microbial Symbionts As a Source For Endogenous Lignocellulolytic Enzymesmentioning

confidence: 99%

Unlocking the potential of insect and ruminant host symbionts for recycling of lignocellulosic carbon with a biorefinery approach: a review

et al. 2021

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Uprising fossil fuel depletion and deterioration of ecological reserves supply have led to the search for alternative renewable and sustainable energy sources and chemicals. Although first generation biorefinery is quite successful commercially in generating bulk of biofuels globally, the food versus fuel debate has necessitated the use of non-edible feedstocks, majorly waste biomass, for second generation production of biofuels and chemicals. A diverse class of microbes and enzymes are being exploited for biofuels production for a series of treatment process, however, the conversion efficiency of wide range of lignocellulosic biomass (LCB) and consolidated way of processing remains challenging. There were lot of research efforts in the past decade to scour for potential microbial candidate. In this context, evolution has developed the gut microbiota of several insects and ruminants that are potential LCB degraders host eco-system to overcome its host nutritional constraints, where LCB processed by microbiomes pretends to be a promising candidate. Synergistic microbial symbionts could make a significant contribution towards recycling the renewable carbon from distinctly abundant recalcitrant LCB. Several studies have assessed the bioprospection of innumerable gut symbionts and their lignocellulolytic enzymes for LCB degradation. Though, some reviews exist on molecular characterization of gut microbes, but none of them has enlightened the microbial community design coupled with various LCB valorization which intensifies the microbial diversity in biofuels application. This review provides a deep insight into the significant breakthroughs attained in enrichment strategy of gut microbial community and its molecular characterization techniques which aids in understanding the holistic microbial community dynamics. Special emphasis is placed on gut microbial role in LCB depolymerization strategies to lignocellulolytic enzymes production and its functional metagenomic data mining eventually generating the sugar platform for biofuels and renewable chemicals production.

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Mining of Novel Microbial Enzymes Using Metagenomics Approach for Efficient Bioremediation

Dwivedi¹,

Gupta²,

Tanveer³

et al. 2023

Genomics Approach to Bioremediation

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An alkaline thermostable laccase from termite gut associated strain of Bacillus stratosphericus

Cited by 15 publications

References 70 publications

An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green

An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green

Unlocking the potential of insect and ruminant host symbionts for recycling of lignocellulosic carbon with a biorefinery approach: a review

Mining of Novel Microbial Enzymes Using Metagenomics Approach for Efficient Bioremediation

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