An airborne actinobacteria Nocardiopsis alba isolated from bioaerosol of a mushroom compost facility

Abstract

Actinobacteria are widely distributed in many environments and represent the most important trigger to the occupant respiratory health. Health complaints, including hypersensitivity pneumonitis of the workers, were recorded in a mushroom compost facility (MCF). The studies on the airborne bacteria were carried out to find a possible microbiological source of these symptoms. Culture analysis of compost bioaerosols collected in different location of the MCF was performed. An assessment of the indoor microbial exposure revealed bacterial flora of bioaerosol in the mushroom compost facility represented by Bacillus, Geobacillus, Micrococcus, Pseudomonas, Staphylococcus spp., and actinobacterial strain with white aerial mycelium. The thermotolerant actinobacterial strain of the same morphology was repeatedly isolated from many locations in MCF: air, compost sample, and solid surface in production hall. On the base of complex morphological, chemotaxonomic, and phylogenetic characteristics, the isolate has been classified as Nocardiopsis alba. Dominant position of N. alba in microbial environment of the mushroom compost facility may represent an indicator microorganism in compost bioaerosol. The bioavailability of N. alba in mushroom compost facility creates potential risk for the health of workers, and the protection of respiratory tract and/or skin is strongly recommended.

Gram-positive aerobic and microaerophilic microorganisms isolated from pathological processes and lesions of horses

“…Another large group which was identified were coryneform microorganisms (34 strains, 7.6% prevalence). Except for C. flavescens which tends to be associated with colour changes on cheese during ripening (Masoud and Jakobsen 2003), the other detected species of corynebacteria and T. pyogenes are associated with doi: 10.17221/107/2016-VETMED animal diseases (Funke et al 1997;Minkin and Shapiro 2004;Songer and Post 2005;Leon-Galvan et al 2015), as are nocardioform microbes (Erol et al 2012b;Pasciak et al 2014) as well as D. congolensis (Wintzer 1999;Songer and Post 2005). Data from several sources indicate that also representatives of the genus Bacillus and Lysinibacillus can be agents of various diseases such as abortion, mastitis, enterotoxaemia and sepsis (Songer and Post 2005;Bednarczyk and Daczkowska-Kozon 2007;Wenzler et al 2015).…”

“…Dermatophilus congolensis is the cause of exudative dermatitis in cattle, sheep and horses (Wintzer 1999), especially in tropical and subtropical regions (Songer and Post 2005). Nocardiopsis alba was discovered in bioaerosol produced by compost for mushroom cultivation and can cause a variety of health problems, including so-called hypersensitivity pneumonia (Pasciak et al 2014). Nocardioform microorganisms including Streptomyces spp.…”

Lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients

“…Bacterial glycolipids are valuable chemical markers characteristic of genus, or species, allowing for identification of the microorganisms (Mordarska, 1985/1986; Paściak et al ., , ). Glycoglycerolipids, especially diglycosyl diglycerides are widespread among Gram‐positive bacteria.…”

Air Quality and Potential Health Risk Impacts of Exposure to Bacterial Aerosol in a Waste Sorting Plant Located in the Mountain Region of Southern Poland, Around Which There Are Numerous Rural Areas

“…Among the dominant species, Neisseria shayeganii was also isolated and identified. This species is mainly associated with the presence of biofilm on human enamel, which indicates its direct origin from hygienic waste [39][40][41][42].…”

“…Bacillus humi was first isolated from an agricultural area in the Netherlands and described in 2005 by Heyrman [49]. However, it was also detected in bioaerosols of a composting plant [41,50]. The strain was considered non-pathogenic [51], but its antibiotic resistance has not yet been studied.…”