An Aggregation-Induced Emission Nanosensor for Real-Time Chemiluminescent Sensing of Light-Independent Intracellular Singlet Oxygen

Abstract

Characterizing the transient ultratrace light-independent intracellular singlet oxygen (1O2), which plays a vital role in multiple biological processes in living organisms, brings about tremendous help for understanding the nature of 1O2-mediated or related bioevents. Nevertheless, an approach to detect the light-independent intracellular 1O2 is hard to find. Herein, we developed a chemiluminescent nanosensor by compacting a great number of TPE-N(Ph)-DBT-PH molecules in one nanostructure via autoaggregation. Taking advantage of the aggregation-induced emission property, this TPE-N(Ph)-DBT-PH nanosensor is highly fluorescent and promises a bright red-light CL and the convenience of mapping in vivo sensor distribution. Experiments demonstrate the nanosensor’s unprecedented selectivity toward 1O2 against other reactive oxygen species. The 3.7 nmol L–1 limit of detection renders this nanosensor with the best-known sensitivity of 1O2 chemical sensors. Meanwhile, fluorescence confocal microscope imaging results suggest that our nanosensor simultaneously targets mitochondria and lysosomes in RAW 264.7 cells via the energy-dependent endocytosis pathway, thereby implying an attractive potential for the detection of intracellular 1O2. Such a potential is demonstrated by detecting 1O2 in RAW 264.7 cells during a lipopolysaccharide and phorbol myristate acetate stimulated respiration burst. This study represents the first approach to detect light-independent intracellular 1O2 during cell bioregulation. Thus, our nanosensor provides an effective tool for investigating the 1O2-related bioprocesses and pathological processes.

Chemiluminescent Conjugated Polymer Nanoparticles for Deep-Tissue Inflammation Imaging and Photodynamic Therapy of Cancer

“…Encouraged by the above imaging results, we further investigated the applicability of PFV-L@PEG-NPs as an inflammation imaging probe in rat models. Here, the rat model of pristane-induced arthritis (PIA) was chosen in this experiment, because it has some similarity to human rheumatoid arthritis . On day 21 of modeling, significant erythema was observed in the hind paws of rats (Figure S33), and chemiluminescence imaging was performed on day 22 (Figure h).…”

“…Here, the rat model of pristane-induced arthritis (PIA) was chosen in this experiment, because it has some similarity to human rheumatoid arthritis. 43 On day 21 of modeling, significant erythema was observed in the hind paws of rats (Figure S33), and chemiluminescence imaging was performed on day 22 (Figure 5h). As shown in Figure 5i,j, after intravenous injection of PFV-L@PEG-NPs, bright chemiluminescence was observed at the inflammatory site in rats with a high signal-to-noise ratio.…”

ROS-Responsive Fluorescent Sensor Array for Precise Diagnosis of Cancer via pH-Controlled Multicolor Gold Nanoclusters

“…ONOO – was obtained by the reaction between NaNO 2 and H 2 O 2 at a molar ratio of 1:5 . NaClO was mixed with H 2 O 2 and stirred for 20 min to obtain 1 O 2 . Superoxide radicals (O 2 ·– ) were prepared by dissolving KO 2 into DMSO solution …”

“…43 NaClO was mixed with H 2 O 2 and stirred for 20 min to obtain 1 O 2 . 44 Superoxide radicals (O 2…”

A de novo zwitterionic strategy of ultra-stable chemiluminescent probes: highly selective sensing of singlet oxygen in FDA-approved phototherapy

“…To date, the gold standard for 1 O 2 determination relies upon the rigorous instrument for feeble phosphorescence signals at 1270 nm and thereby is merely utilized for in vitro application. 15 Hence, there is an urgent demand to develop a design strategy for highly sensitive and selective probes for the in vivo detection of singlet oxygen.…”