An agent in the Aedes aegypti cell line (Peleg) which causes fusion of Aedes albopictus cells

Stollar, Victor; Thomas, Virginia L.

doi:10.1016/0042-6822(75)90113-0

Cited by 222 publications

(184 citation statements)

References 14 publications

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“…aegypti cell line in 1975. 31 The CFAV has subsequently been found in Ae. aegypti populations in many parts of the world.…”

Section: Resultsmentioning

confidence: 99%

Insect-Specific Viruses Detected in Laboratory Mosquito Colonies and Their Potential Implications for Experiments Evaluating Arbovirus Vector Competence

Bolling¹,

Vasilakis²,

Guzmán³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. Recently, there has been a dramatic increase in the detection and characterization of insect-specific viruses in field-collected mosquitoes. Evidence suggests that these viruses are ubiquitous in nature and that many are maintained by vertical transmission in mosquito populations. Some studies suggest that the presence of insect-specific viruses may inhibit replication of a super-infecting arbovirus, thus altering vector competence of the mosquito host. Accordingly, we screened our laboratory mosquito colonies for insect-specific viruses. Pools of colony mosquitoes were homogenized and inoculated into cultures of Aedes albopictus (C6/36) cells. The infected cells were examined by electron microscopy and deep sequencing was performed on RNA extracts. Electron micrograph images indicated the presence of three different viruses in three of our laboratory mosquito colonies. Potential implications of these findings for vector competence studies are discussed.

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“…aegypti cell line in 1975. 31 The CFAV has subsequently been found in Ae. aegypti populations in many parts of the world.…”

Section: Resultsmentioning

confidence: 99%

Insect-Specific Viruses Detected in Laboratory Mosquito Colonies and Their Potential Implications for Experiments Evaluating Arbovirus Vector Competence

Bolling¹,

Vasilakis²,

Guzmán³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…The mosquito-specific flaviviruses can be divided into two distinct groups. The first group includes cell fusing agent, Kamiti River virus, and Culex flaviviruses, which are distantly related phylogenetically to the main branch of mosquito-and tick-borne pathogenic vertebrate viruses (19)(20)(21). These viruses likely represent an ancestral lineage that could only infect invertebrates and subsequently gained the ability to infect vertebrates.…”

Section: Discussionmentioning

confidence: 99%

Eilat virus, a unique alphavirus with host range restricted to insects by RNA replication

Nasar

Palacios

Gorchakov

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

166

189

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Most alphaviruses and many other arboviruses are mosquito-borne and exhibit a broad host range, infecting many different vertebrates including birds, rodents, equids, humans, and nonhuman primates. Consequently, they can be propagated in most vertebrate and insect cell cultures. This ability of arboviruses to infect arthropods and vertebrates is usually essential for their maintenance in nature. However, several flaviviruses have recently been described that infect mosquitoes but not vertebrates, although the mechanism of their host restriction has not been determined. Here we describe a unique alphavirus, Eilat virus (EILV), isolated from a pool of Anopheles coustani mosquitoes from the Negev desert of Israel. Phylogenetic analyses placed EILV as a sister to the Western equine encephalitis antigenic complex within the main clade of mosquito-borne alphaviruses. Electron microscopy revealed that, like other alphaviruses, EILV virions were spherical, 70 nm in diameter, and budded from the plasma membrane of mosquito cells in culture. EILV readily infected a variety of insect cells with little overt cytopathic effect. However, in contrast to typical mosquito-borne alphaviruses, EILV could not infect mammalian or avian cell lines, and viral as well as RNA replication could not be detected at 37°C or 28°C. Evolutionarily, these findings suggest that EILV lost its ability to infect vertebrate cells. Thus, EILV seems to be mosquito-specific and represents a previously undescribed complex within the genus Alphavirus. Reverse genetic studies of EILV may facilitate the discovery of determinants of alphavirus host range that mediate disease emergence.evolution | Togavirus

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“…Experiments to ascertain the identity of the Berne P138/72 isolate included virus neutralization, indirect immunofluorescence and ELISA. Immune sera directed against arthropod-borne and non-arthropodborne members of the Togaviridae [Semliki Forest, Sindbis, rubella, bovine diarrhoea, equine arteritis, Aedes albopictus cell-fusing virus (Stollar & Thomas, 1975)], Coronaviridae (mouse hepatitis virus strain A59, porcine transmissible gastroenteritis and avian infectious bronchitis virus), Orthomyxoviridae (influenza C virus) and Herpesviridae (equine herpesvirus type 1) families were employed. In addition, sera from gnotobiotic calves after infection with two serotypes of the recently described Breda virus (Woode et al, 1982) as well as a non-immune gnotobiotic calf serum were tested; from Dr A. Moussa, Laboratoire National de Pathologic Bovine, Lyon, France, immune sera against another new virus isolate (Lyon-4 virus) were obtained and included in the experiments.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Purification and Partial Characterization of a New Enveloped RNA Virus (Berne Virus)

Weiss

Steck

Horzinek

1983

Journal of General Virology

118

168

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SUMMARYIn Berne (Switzerland), a virus was isolated from a horse which was found to be serologically unrelated to known equine viruses. Its growth was unaffected by iododeoxyuridine and it was inactivated by organic solvents. A purification procedure involving ammonium sulphate precipitation and sucrose gradient equilibrium centrifugation was developed and viral activities were monitored using infectivity and enzyme-linked immunosorbent assays. Purified virions of density 1-16 g/ml were shown by negative staining electron microscopy to be roughly spherical and to measure 120 to 140 nm in diameter; projections (peplomers, about 20 nm long) were identified on the virion surface. In thin sections, an envelope and an elongated core structure could be distinguished. The core, measuring about 23 nm across and 104 nm in length, appears to assume a rod-, crescent-or open ring-shape within the envelope. It has a tubular structure and shows a transverse striation (periodicity 4.5 nm). Budding at the plasma membrane was observed. Berne virus is considered as a representative of a hitherto undefined family of widespread animal viruses serologically related to recent bovine isolates in Ames, Iowa (U.S.A.) and Lyon (France).

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An agent in the Aedes aegypti cell line (Peleg) which causes fusion of Aedes albopictus cells

Cited by 222 publications

References 14 publications

Insect-Specific Viruses Detected in Laboratory Mosquito Colonies and Their Potential Implications for Experiments Evaluating Arbovirus Vector Competence

Insect-Specific Viruses Detected in Laboratory Mosquito Colonies and Their Potential Implications for Experiments Evaluating Arbovirus Vector Competence

Eilat virus, a unique alphavirus with host range restricted to insects by RNA replication

Purification and Partial Characterization of a New Enveloped RNA Virus (Berne Virus)

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