An agar well punching-sucking device for diffusion assays

Purification, characterization, and mode of action of Sakacin ZFM225, a novel bacteriocin from Lactobacillus sakei ZFM225

“…Cell-free supernatant (CFS) was separated from the fermentation broth at 8000 rpm for 30 min at 4 °C. Determination of antimicrobial activity was tested by agar well diffusion assay [ 19 ]. The indicator strain M. luteus 10209 came from China Center for Industrial Culture Collection (CICC, Beijing, China) with the collection number of CICC 10209 and was incubated at 30 °C for 12 h.…”

Factors affecting the production of thermonuclease by Staphylococcus aureus Z88

“…The sizes of the wells varied from 5.00 mm to 5.50 mm depending on the experiment. Hallynck and Pijck (1978) noted that cutting wells in agar with a cork borer can lead to wells with unevenly cut edges. To remove this potential source of inaccuracy, the measurement referred to as cleared zone (CZ) was de veloped.…”