An affinity column for phospholipase A2 based on immobilised acylaminophospholipid analogues

Purification of Phospholipases A2 from American Snake Venoms

“…Rock and Snyder [50] were the first ones to use phospholipid analogous to build a bioaffinity matrix [Rac-1-(9-carboxy)-nonil-2-exadecilglycero-3-phosphocoline]. In addition to them, Dijkman [51] described the synthesis of an analogous of acylamino phospholipid[(R)-1-deoxy-1-thio-(ω-carboxy-undecyl)-2-deoxy-(ndecanoylamino)-3-glycerophosphocholine] which was coupled to a Sepharose 6B® resin containing a spacer arm. With the use of this resin it was possible to purify phospholipases from horse pancreas, and venoms from Naja melanonleuca and Crotallus adamanteus.…”

Unilamellar liposomes covalently coupled on silica gel for liquid chromatography

“…Immobilized liposomes have been successfully used as chromatographic stationary phases to study drug-membrane partitioning [4,5,6,7,8], protein purification [9,10], protein-membrane interaction [11], and bioaffinity measurements [12]. They have also received increased attention in recent years as a powerful tool to study solutemembrane interactions.…”