An advanced uracil DNA glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP) technique used in the sensitive and specific detection of Cryptosporidium parvum , Cryptosporidium hominis , and Cryptosporidium meleagridis in AIDS patients

A Review of the Prevalence and Diagnostic Points of Cryptosporidium Species in Immunocompromised and Healthy Human Samples in Iran

“…Moreover, one study in Iran used the uracil DNA glycosylasesupplemented loop-mediated isothermal amplification technique based on the target gene S-adenosyl methionine synthetase from Cryptosporidium gene to detect Cryptosporidium species in human immunodeficiency virus (HIV)-positive patients. This study tried to eliminate false-positive results following contamination (57).…”

Accurate and rapid detection of Fasciola hepatica copro‐DNA in sheep using loop‐mediated isothermal amplification (LAMP) technique

“…F3, FIP (F1c + F2), BIP (B1c + B2) and B3] binding to six independent sites in the target sequence (Fallahi et al, 2020;Ghodsian et al, 2019;Hanifehpour et al, 2019;Parida et al, 2008). An appropriate selection of the target gene and the optimal design of turn-back primers have successful LAMP result (Fallahi et al, 2017(Fallahi et al, , 2018Ghodrati et al, 2017;Hoa Le et al, 2012) and cause visible turbidity (Mori & Notomi, 2009). This technique is carried out using inexpensive equipment such as a regular water bath or a heating block, and its products are easily seen with the eye or visualized by the addition of fluorescent dyes such as SYBR green I, with or without using a UV lamp (Mori et al, 2001).…”

A pond-side test for Guinea worm: Development of a loop-mediated isothermal amplification (LAMP) assay for detection of Dracunculus medinensis

“…In recent years the development of isothermal DNA amplification approaches (amplification at a single temperature) such as Loop mediated AMPlification (LAMP) provide the potential for achieving the same levels of analytical precision in remote and under-resourced locations, including directly in the field for the detection of parasites (e.g. Tong et al, 2015 ; Cook et al, 2015 ; Lodh et al, 2017 ; Fallahi et al, 2018 ). No thermal cycling is required, enabling the use of simple, battery powered, portable equipment such as the Genie III (Optisense, Horsham, UK) and the enzymes used are sufficiently robust that DNA template does not need to be purified to achieve amplification, something that is not possible using PCR methods.…”