Flowing plasma jets are increasingly investigated and used for surface treatments, including biological matter, and as soft ionization sources for mass spectrometry. They have the characteristic capability to transport energy from the plasma excitation region to the flowing afterglow, and therefore to a distant application surface, in a controlled manner. The ability to transport and deposit energy into a specimen is related to the actual energy transport mechanism. In case of a flowing helium plasma, the energy in the flowing afterglow may be carried by metastable helium atoms and long-lived helium dimer ions. In this work a systematic investigation of the optical and spectroscopic characteristics of a supersonic flowing helium plasma in vacuum and its afterglow as function of the helium gas density is presented. The experimental data are compared with numerical modeling of the plasma excitation and helium dimer ion formation supported by a Computational Fluid Dynamic simulation of the helium jet. The results indicate that the plasma afterglow is effectively due to helium dimer ions recombination via a three-body reaction.The study of noble gas flowing plasma has gained much attention in recent years due to its impact on the application of cold plasma jet and torches for soft ionization and mass spectrometry 1-10 .For a flowing helium plasma the dynamics of the energy transport in the afterglow is very complicated due to the peculiar helium plasma characteristics: presence of highly energetic metastable states and energetic excited states close to the ionization limit, as well as the prominent tendency, at increasing particle density, to form helium dimer ions 11,12 . The actual mechanisms and reactions governing the energy and charge transport from the plasma excitation region to the subsequent stream is of importance to understand and optimize the flowing plasma jets performances. Flowing plasma jets are characterized and optimized by playing on the gas flow rate, the plasma excitation power, the distance to the sample to be probed and the nozzle shape [13][14][15] .There are two main mechanisms supposedly underlying the energy transport in flowing helium plasma jets and the subsequent reactive nitrogen ion formation in ambient air.One mechanism is related to the high excited state energy of metastable helium atoms (more than 20 eV) which are formed during the plasma excitation, eventually from cascade transitions from higher excited states. The helium metastable atoms, He m , have long lifetime and therefore, in principle, can live in the afterglow, giving rise eventually to nitrogen ion formation in air through de-excitation and penning ionization in air 11 ,On the other side, at increasing particle number density, the excited helium atoms and the helium ions are rapidly and efficiently transformed into helium dimer ions, + He 2 , which have a long lifetime, since they do not posses a