An advanced biphasic porous and injectable scaffold displays a fine balance between mechanical strength and remodeling capabilities essential for cartilage regeneration

Zavala, Gabriela; Viafara-García, Sergio M.; Novoa, Javier; Hidalgo, Carmen; Contardo, Ingrid; Díaz-Calderón, Paulo; Alejandro González-Arriagada, Wilfredo; Khoury, Maroun; Acevedo, Juan Pablo

doi:10.1039/d3bm00703k

Biomater. Sci.

2023

DOI: 10.1039/d3bm00703k

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An advanced biphasic porous and injectable scaffold displays a fine balance between mechanical strength and remodeling capabilities essential for cartilage regeneration

Gabriela Zavala,

Sergio M. Viafara-García,

Javier Novoa

et al.

Abstract: An important challenge in tissue engineering is the regeneration of functional articular cartilage (AC).

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2024

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Development of an alginate–chitosan biopolymer composite with dECM bioink additive for organ-on-a-chip articular cartilage

Upadhyay,

Kolla,

Maredupaka

et al. 2024

Sci Rep

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In vitro use of articular cartilage on an organ-on-a-chip (OOAC) via microfluidics is challenging owing to the dense extracellular matrix (ECM) composed of numerous protein moieties and few chondrocytes, which has limited proliferation potential and microscale translation. Hence, this study proposes a novel approach for using a combination of biopolymers and decellularised ECM (dECM) as a bioink additive in the development of scalable OOAC using a microfluidic platform. The bioink was tested with native chondrocytes and mesenchymal stem cell-induced chondrocytes using biopolymers of alginate and chitosan composite hydrogels. Two-dimensional (2D) and three-dimensional (3D) biomimetic tissue construction approaches have been used to characterise the morphology and cellular marker expression (by histology and confocal laser scanning microscopy), viability (cell viability dye using flow cytometry), and genotypic expression of ECM-specific markers (by quantitative PCR). The results demonstrated that the bioink had a significant impact on the increase in phenotypic and genotypic expression, with a statistical significance level of p < 0.05 according to Student’s t-test. The use of a cell-laden biopolymer as a bioink optimised the niche conditions for obtaining hyaline-type cartilage under culture conditions, paving the way for testing mechano-responsive properties and translating these findings to a cartilage-on-a-chip microfluidics system.

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Development of an alginate–chitosan biopolymer composite with dECM bioink additive for organ-on-a-chip articular cartilage

Upadhyay,

Kolla,

Maredupaka

et al. 2024

Sci Rep

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show abstract

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An advanced biphasic porous and injectable scaffold displays a fine balance between mechanical strength and remodeling capabilities essential for cartilage regeneration

Abstract: An important challenge in tissue engineering is the regeneration of functional articular cartilage (AC).

Cited by 1 publication

References 46 publications

Development of an alginate–chitosan biopolymer composite with dECM bioink additive for organ-on-a-chip articular cartilage

Development of an alginate–chitosan biopolymer composite with dECM bioink additive for organ-on-a-chip articular cartilage

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