An adolescent with multi-organ involvement from typhoid fever

Kumwenda, Mercy; Tam, Pui Ying Iroh

doi:10.4314/mmj.v31i2.10

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…The ileum is the most affected intestinal segment (Table 3). According to Kumwenda et al [16], hypertrophy of the Peyer's patches causes obstruction of lumen and constipation. In severe cases, necrosis of Peyer patches lead to ulceration, bleeding and eventually perforation of the terminal ileum.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Efficacy of Haematoxylin & Eosin (H&E) and Periodic Acid Schiff (PAS) Staining Techniques in the Diagnosis of Typhoid Intestinal Perforation (TIP) among Patients Attending Federal Medical Centre, Keffi Nasarawa State, Nigeria

Gambari,

Erameh,

Ugbomoiko

et al. 2023

IJTDH

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Aim of the Study: To compare the efficacy of H&E and PAS staining techniques in the demonstration of TIP among patients attending Federal Medical Centre Keffi, Nasarawa state. Study Design: The study is a retrospective study, a total of 129 paraffin-embedded tissue blocks of patients who were diagnosed of TIP between January 1st 2013 and December 31st 2022 were retrieved from the archives of Histopathology laboratory and examined. Ten patients (10) with incomplete data were excluded. Methodology: A semi structured questionnaire was used to collect demographic data. The tissue blocks were sectioned at 4um and stained using Haematoxylin and Eosin (H&E) staining technique and Periodic Acid Schiff (PAS) technique. The microscopic examination was done using x10 and x40 objectives of the Olympus light microscope. Results: The study showed that males were more affected with TIP than females with ratio 1.6: 1. Ages of patients ranged between 6 months-77 years with mean age of 24.8+1.87 years. The highest number of TIP cases was recorded in December. The ileal segment was the most perforated among the intestinal segments. The socioeconomic status of patients, availability of potable water supply as well as hygiene and sanitary practices were discovered to be key factors in typhoid fever infection and treatment outcomes. Conclusion: Although, both PAS and H&E staining techniques showed favorable features, the PAS technique gave better contrast and also revealed the perforation sites better than H&E at P<0.001. Hence, the PAS technique should be done alongside the H&E in the laboratory investigation of intestinal tissues.

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Section: Discussionmentioning

confidence: 99%