An Adenovirus Isolated from the Feces of Mice

Sugiyama, Takiko; Hashimoto, Kazuo; Sasaki, Satoshi

doi:10.1111/j.1348-0421.1967.tb00318.x

Cited by 28 publications

(18 citation statements)

References 19 publications

(20 reference statements)

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“…As already reported [15,36], the virus was observed to grow in cells, scattered in the epithelial layer of the mucous membrane of the small intestine, for 2 to 3 weeks. After such an enteric infection, an intestinal resistance against the viral growth could be established, while there was no resistance with mere elevation of serum antibody.…”

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confidence: 55%

Intestinal Resistance in the Experimental Enteric Infection of Mice with a Mouse Adenovirus

Hashimoto

Okada

Tajiri

et al. 1973

Japanese Journal of Microbiology

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The influence of cyclophosphamide (Cy) on the establishment and duration of the intestinal resistance against enteric infection with a mouse adenovirus, strain K87, was examined in inbred mice, strain DK1. When Cy (40 mg/kg/day) was administered to mice for 17 days from the time of virus challenge, a clear prolongation of viral growth and a delayed appearance of neutralizing (NT) antibody in the intestinal wall as well as in the serum were observed. When Cy (40 mg/kg/day, for 14 days) was administered after cessation of viral growth (4 to 6 weeks after virus challenge) and part of the mice were rechallenged with the virus, titers of NT antibody and immunoglobulins became significantly lower than those in control mice not treated with Cy, and regrowth of the virus was observed in eight out of twenty-five Cy-treated mice, regardless of the presence or absence of rechallenge. In this experiment, antibody titers in the intestinal contents of eight virus-positive mice were significantly lower than those of the remaining seventeen virus-negative mice. The time when the decrease of intestinal NT antibody was maximum coincided with the time of the maximal frequency of viral regrowth. It was discussed that these facts might present an evidence to support the idea that the intestinal resistance was acquired through local NT antibody belonging to-IgA in the intestinal tract.

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confidence: 55%

Intestinal Resistance in the Experimental Enteric Infection of Mice with a Mouse Adenovirus

Hashimoto

Okada

Tajiri

et al. 1973

Japanese Journal of Microbiology

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“…Two AdVs have been so far isolated from mice: FL (14) and K87 (15,29), which have meanwhile been designated as MAdV-1 (for murine AdV type 1) and MAdV-2 and represent distinct species MAdV-A and MAdV-B, respectively. MAdV-2 grows very inefficiently in cell cultures (13), and experimental infection of mice leads to diarrhea (29).…”

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A Novel Cardiotropic Murine Adenovirus Representing a Distinct Species of Mastadenoviruses

Klempa

Krüger

Auste

et al. 2009

J Virol

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During cell culture isolation experiments to recover Dobrava hantavirus from a suspension of liver from a striped field mouse (Apodemus agrarius), an unknown virus was coisolated. Atypically for hantaviruses, it had extensive cytopathic effects. Using a random PCR approach, it was identified as a novel murine adenovirus, MAdV-3 (for MAdV type 3). A plaque-purified virus clone was prepared and further characterized. The complete genome sequence of MAdV-3 was determined to be 30,570 bp in length. Sequence comparisons to other adenovirus species revealed highest similarity to MAdV-1, the representative of the murine adenovirus A species. However, substantial differences were found in the E1, E3, and E4 genomic regions. The phylogenetic distance of MAdV-3 amino acid sequences for pVIII, protease, polymerase, and hexon from MAdV-1 is markedly higher than 0.1 exchange per position, and, based on our cross-neutralization experiments, MAdV-3 and MAdV-1 can be regarded as different serotypes. Therefore, we propose to classify MAdV-3 as the first isolate of a novel adenovirus species, designated murine adenovirus C (MAdV-C). The novel MAdV-3 virus is not only genetically and serologically distinct from MAdV-1 but also shows a unique organ tropism in infected mice. In contrast to MAdV-1, the virus was not detectable in brain but predominantly infected heart tissue. Thus, infection of mice with cardiotropic MAdV-3 might be an interesting animal model of adenovirus-induced myocarditis.

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“…After inoculation, the virus is excreted with the feces for 3 weeks or longer as will be described in a future report [23]. …”

Section: Pathogenicity Of Strain K87mentioning

confidence: 99%

An Adenovirus Isolated from the Feces of Mice

Hashimoto

Sugiyama

Sasaki

1966

Japanese Journal of Microbiology

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Cytopathogenic agents have been isolated in a search for viruses in the feces of apparently healthy mice (an inbred strain DK1) by using mouse kidney tissue culture. This report is concerned with the identification of strain K87, one of our isolates, as an adenovirus. Strain K87 was cytopathogenic to mouse kidney tissue culture but not to monkey kidney tissue culture, FL, and HeLa cells. The K87 strain was not able to grow in bacterial media and was resistant to penicillin, streptomycin and tetracycline. 5‐Bromodeoxyuridine inhibited the occurrence of the cytopathogenic effect and virus replication in infected cells and the inhibitory effect was reversed by thymidine, suggesting that the virus contains DNA. Strain K87 was resistant to ethyl‐ether but did not have the property of cationic stabilization to thermal inactivation. Electronmicroscopic observations of thin‐sections of the K87‐infected cells showed virus particles in crystalline array in the nuclei. Each virus particle was an icosahedron of about 75 mμ in diameter composed of 252 capsomeres, and without an envelope. By the complement fixation test, K87 was related serologically to a human adenovirus. All the facts indicate that strain K87 belongs to the adenovirus group. The problem whether strain K87 may be a new mouse adenovirus with pathogenicity and antigenicity different from that of a mouse adenovirus previously reported by Hartley and Rowe is discussed.

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An Adenovirus Isolated from the Feces of Mice

Cited by 28 publications

References 19 publications

Intestinal Resistance in the Experimental Enteric Infection of Mice with a Mouse Adenovirus

Intestinal Resistance in the Experimental Enteric Infection of Mice with a Mouse Adenovirus

A Novel Cardiotropic Murine Adenovirus Representing a Distinct Species of Mastadenoviruses

An Adenovirus Isolated from the Feces of Mice

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