An adapted method for Cas9-mediated editing reveals the species-specific role of β-glucoside utilization driving competition betweenKlebsiellaspecies
Éva d. H. Almási,
Nele Knischewski,
Lisa Osbelt
et al.
Abstract:Cas9-based gene editing tools have revolutionized genetics, enabling the fast and precise manipulation of diverse bacterial species. However, widely applicable genetic tools for non-model gut bacteria are unavailable. Here, we present a two-plasmid Cas9-based system designed for gene deletion and knock-in complementation in three members of theKlebsiella oxytocaspecies complex (KoSC), which we applied to study the genetic factors underlying the role of these bacteria in competition againstKlebsiella pneumoniae… Show more
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